Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4327 and Pf1N1B4_4328 are separated by 26 nucleotides Pf1N1B4_4328 and Pf1N1B4_4329 are separated by 267 nucleotides Pf1N1B4_4329 and Pf1N1B4_4330 are separated by 21 nucleotides
Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785
_4327
Pf1N1B4_4328 - Agmatine deiminase (EC 3.5.3.12), at 4,788,812 to 4,789,936
_4328
Pf1N1B4_4329 - Acyl dehydratase, at 4,790,204 to 4,790,689
_4329
Pf1N1B4_4330 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 4,790,711 to 4,791,553
_4330
Position (kb)
4788
4789
4790 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.043 kb on - strand at 4788.043 kb on - strand at 4788.072 kb on + strand, within Pf1N1B4_4327 at 4788.073 kb on - strand, within Pf1N1B4_4327 at 4788.099 kb on + strand at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.249 kb on + strand, within Pf1N1B4_4327 at 4788.250 kb on - strand, within Pf1N1B4_4327 at 4788.320 kb on - strand, within Pf1N1B4_4327 at 4788.327 kb on + strand, within Pf1N1B4_4327 at 4788.332 kb on - strand, within Pf1N1B4_4327 at 4788.543 kb on + strand, within Pf1N1B4_4327 at 4788.544 kb on - strand, within Pf1N1B4_4327 at 4788.672 kb on + strand at 4788.672 kb on + strand at 4788.673 kb on - strand at 4788.673 kb on - strand at 4788.690 kb on + strand at 4789.061 kb on + strand, within Pf1N1B4_4328 at 4789.139 kb on + strand, within Pf1N1B4_4328 at 4789.140 kb on - strand, within Pf1N1B4_4328 at 4789.268 kb on - strand, within Pf1N1B4_4328 at 4789.351 kb on + strand, within Pf1N1B4_4328 at 4789.755 kb on - strand, within Pf1N1B4_4328 at 4789.810 kb on + strand, within Pf1N1B4_4328 at 4789.811 kb on - strand, within Pf1N1B4_4328 at 4789.860 kb on + strand at 4789.861 kb on - strand at 4790.121 kb on - strand at 4790.210 kb on - strand at 4790.527 kb on + strand, within Pf1N1B4_4329 at 4790.575 kb on - strand, within Pf1N1B4_4329 at 4790.605 kb on + strand, within Pf1N1B4_4329 at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.641 kb on + strand at 4790.867 kb on + strand, within Pf1N1B4_4330 at 4790.909 kb on - strand, within Pf1N1B4_4330
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,787,850 + Pf1N1B4_4327 0.17 -1.1 4,787,850 + Pf1N1B4_4327 0.17 +1.0 4,787,850 + Pf1N1B4_4327 0.17 +0.9 4,787,850 + Pf1N1B4_4327 0.17 -1.4 4,788,042 + Pf1N1B4_4327 0.34 +0.4 4,788,042 + Pf1N1B4_4327 0.34 +0.3 4,788,042 + Pf1N1B4_4327 0.34 -1.6 4,788,042 + Pf1N1B4_4327 0.34 -0.9 4,788,043 - +1.3 4,788,043 - -1.9 4,788,072 + Pf1N1B4_4327 0.37 +0.2 4,788,073 - Pf1N1B4_4327 0.37 +0.1 4,788,099 + -0.9 4,788,201 - Pf1N1B4_4327 0.48 +0.7 4,788,201 - Pf1N1B4_4327 0.48 -0.6 4,788,249 + Pf1N1B4_4327 0.53 +0.2 4,788,250 - Pf1N1B4_4327 0.53 -2.5 4,788,320 - Pf1N1B4_4327 0.59 -0.3 4,788,327 + Pf1N1B4_4327 0.60 +0.3 4,788,332 - Pf1N1B4_4327 0.60 +2.1 4,788,543 + Pf1N1B4_4327 0.79 -0.3 4,788,544 - Pf1N1B4_4327 0.79 -0.1 4,788,672 + -1.5 4,788,672 + -0.1 4,788,673 - +0.9 4,788,673 - -1.3 4,788,690 + +1.1 4,789,061 + Pf1N1B4_4328 0.22 -0.3 4,789,139 + Pf1N1B4_4328 0.29 -0.8 4,789,140 - Pf1N1B4_4328 0.29 -0.5 4,789,268 - Pf1N1B4_4328 0.41 -0.9 4,789,351 + Pf1N1B4_4328 0.48 -1.3 4,789,755 - Pf1N1B4_4328 0.84 -1.6 4,789,810 + Pf1N1B4_4328 0.89 -0.0 4,789,811 - Pf1N1B4_4328 0.89 -1.6 4,789,860 + -0.4 4,789,861 - +0.1 4,790,121 - -1.6 4,790,210 - +1.3 4,790,527 + Pf1N1B4_4329 0.66 +2.7 4,790,575 - Pf1N1B4_4329 0.76 -0.3 4,790,605 + Pf1N1B4_4329 0.83 -0.3 4,790,641 + +1.7 4,790,641 + -1.6 4,790,641 + +0.7 4,790,641 + -1.2 4,790,867 + Pf1N1B4_4330 0.19 -0.3 4,790,909 - Pf1N1B4_4330 0.23 +1.4
Or see this region's nucleotide sequence