Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4321

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4319 and Pf1N1B4_4320 overlap by 4 nucleotidesPf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotidesPf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotidesPf1N1B4_4322 and Pf1N1B4_4323 are separated by 15 nucleotides Pf1N1B4_4319 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 4,781,337 to 4,782,104 _4319 Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928 _4320 Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719 _4321 Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567 _4322 Pf1N1B4_4323 - hypothetical protein, at 4,784,583 to 4,784,744 _4323 Pf1N1B4_4324 - Glycine cleavage system transcriptional activator, at 4,784,714 to 4,785,580 _4324 Position (kb) 4782 4783 4784Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4782.038 kb on - strandat 4782.038 kb on - strandat 4782.156 kb on - strandat 4782.156 kb on - strandat 4782.218 kb on + strandat 4782.218 kb on + strand, within Pf1N1B4_4320at 4782.218 kb on + strand, within Pf1N1B4_4320at 4782.219 kb on - strand, within Pf1N1B4_4320at 4782.219 kb on - strand, within Pf1N1B4_4320at 4782.301 kb on - strand, within Pf1N1B4_4320at 4782.423 kb on + strand, within Pf1N1B4_4320at 4782.447 kb on + strand, within Pf1N1B4_4320at 4782.447 kb on + strand, within Pf1N1B4_4320at 4782.452 kb on - strand, within Pf1N1B4_4320at 4782.452 kb on - strand, within Pf1N1B4_4320at 4783.150 kb on - strandat 4783.235 kb on + strand, within Pf1N1B4_4321at 4783.236 kb on - strand, within Pf1N1B4_4321at 4783.762 kb on + strandat 4783.763 kb on - strandat 4783.862 kb on + strand, within Pf1N1B4_4322at 4784.087 kb on - strandat 4784.087 kb on - strand, within Pf1N1B4_4322at 4784.118 kb on - strand, within Pf1N1B4_4322at 4784.118 kb on - strandat 4784.122 kb on + strand, within Pf1N1B4_4322at 4784.419 kb on + strand, within Pf1N1B4_4322at 4784.582 kb on + strandat 4784.582 kb on + strandat 4784.715 kb on + strandat 4784.715 kb on + strandat 4784.716 kb on - strandat 4784.716 kb on - strandat 4784.716 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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4,782,038 - -0.5
4,782,038 - -1.3
4,782,156 - -2.5
4,782,156 - -1.9
4,782,218 + +0.7
4,782,218 + Pf1N1B4_4320 0.14 +1.5
4,782,218 + Pf1N1B4_4320 0.14 -0.3
4,782,219 - Pf1N1B4_4320 0.14 +0.7
4,782,219 - Pf1N1B4_4320 0.14 +0.1
4,782,301 - Pf1N1B4_4320 0.24 -0.9
4,782,423 + Pf1N1B4_4320 0.39 -0.5
4,782,447 + Pf1N1B4_4320 0.42 +0.5
4,782,447 + Pf1N1B4_4320 0.42 +1.1
4,782,452 - Pf1N1B4_4320 0.42 -1.6
4,782,452 - Pf1N1B4_4320 0.42 -1.9
4,783,150 - +0.7
4,783,235 + Pf1N1B4_4321 0.39 -0.6
4,783,236 - Pf1N1B4_4321 0.39 +1.6
4,783,762 + +3.3
4,783,763 - -0.3
4,783,862 + Pf1N1B4_4322 0.17 +0.1
4,784,087 - +1.1
4,784,087 - Pf1N1B4_4322 0.44 +1.3
4,784,118 - Pf1N1B4_4322 0.47 +0.2
4,784,118 - +2.3
4,784,122 + Pf1N1B4_4322 0.48 -0.6
4,784,419 + Pf1N1B4_4322 0.83 -0.6
4,784,582 + -0.7
4,784,582 + +0.7
4,784,715 + -1.5
4,784,715 + -0.1
4,784,716 - +1.0
4,784,716 - -1.6
4,784,716 - -0.5

Or see this region's nucleotide sequence