Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4319 and Pf1N1B4_4320 overlap by 4 nucleotides Pf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotides Pf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotides Pf1N1B4_4322 and Pf1N1B4_4323 are separated by 15 nucleotides
Pf1N1B4_4319 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 4,781,337 to 4,782,104
_4319
Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928
_4320
Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719
_4321
Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567
_4322
Pf1N1B4_4323 - hypothetical protein, at 4,784,583 to 4,784,744
_4323
Pf1N1B4_4324 - Glycine cleavage system transcriptional activator, at 4,784,714 to 4,785,580
_4324
Position (kb)
4782
4783
4784 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4782.038 kb on - strand at 4782.038 kb on - strand at 4782.156 kb on - strand at 4782.156 kb on - strand at 4782.218 kb on + strand at 4782.218 kb on + strand, within Pf1N1B4_4320 at 4782.218 kb on + strand, within Pf1N1B4_4320 at 4782.219 kb on - strand, within Pf1N1B4_4320 at 4782.219 kb on - strand, within Pf1N1B4_4320 at 4782.301 kb on - strand, within Pf1N1B4_4320 at 4782.423 kb on + strand, within Pf1N1B4_4320 at 4782.447 kb on + strand, within Pf1N1B4_4320 at 4782.447 kb on + strand, within Pf1N1B4_4320 at 4782.452 kb on - strand, within Pf1N1B4_4320 at 4782.452 kb on - strand, within Pf1N1B4_4320 at 4783.150 kb on - strand at 4783.235 kb on + strand, within Pf1N1B4_4321 at 4783.236 kb on - strand, within Pf1N1B4_4321 at 4783.762 kb on + strand at 4783.763 kb on - strand at 4783.862 kb on + strand, within Pf1N1B4_4322 at 4784.087 kb on - strand at 4784.087 kb on - strand, within Pf1N1B4_4322 at 4784.118 kb on - strand, within Pf1N1B4_4322 at 4784.118 kb on - strand at 4784.122 kb on + strand, within Pf1N1B4_4322 at 4784.419 kb on + strand, within Pf1N1B4_4322 at 4784.582 kb on + strand at 4784.582 kb on + strand at 4784.715 kb on + strand at 4784.715 kb on + strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.716 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,782,038 - -0.5 4,782,038 - -1.3 4,782,156 - -2.5 4,782,156 - -1.9 4,782,218 + +0.7 4,782,218 + Pf1N1B4_4320 0.14 +1.5 4,782,218 + Pf1N1B4_4320 0.14 -0.3 4,782,219 - Pf1N1B4_4320 0.14 +0.7 4,782,219 - Pf1N1B4_4320 0.14 +0.1 4,782,301 - Pf1N1B4_4320 0.24 -0.9 4,782,423 + Pf1N1B4_4320 0.39 -0.5 4,782,447 + Pf1N1B4_4320 0.42 +0.5 4,782,447 + Pf1N1B4_4320 0.42 +1.1 4,782,452 - Pf1N1B4_4320 0.42 -1.6 4,782,452 - Pf1N1B4_4320 0.42 -1.9 4,783,150 - +0.7 4,783,235 + Pf1N1B4_4321 0.39 -0.6 4,783,236 - Pf1N1B4_4321 0.39 +1.6 4,783,762 + +3.3 4,783,763 - -0.3 4,783,862 + Pf1N1B4_4322 0.17 +0.1 4,784,087 - +1.1 4,784,087 - Pf1N1B4_4322 0.44 +1.3 4,784,118 - Pf1N1B4_4322 0.47 +0.2 4,784,118 - +2.3 4,784,122 + Pf1N1B4_4322 0.48 -0.6 4,784,419 + Pf1N1B4_4322 0.83 -0.6 4,784,582 + -0.7 4,784,582 + +0.7 4,784,715 + -1.5 4,784,715 + -0.1 4,784,716 - +1.0 4,784,716 - -1.6 4,784,716 - -0.5
Or see this region's nucleotide sequence