Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4313 and Pf1N1B4_4314 are separated by 233 nucleotides Pf1N1B4_4314 and Pf1N1B4_4315 are separated by 43 nucleotides Pf1N1B4_4315 and Pf1N1B4_4316 are separated by 212 nucleotides
Pf1N1B4_4313 - putative membrane protein, at 4,773,543 to 4,774,268
_4313
Pf1N1B4_4314 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 4,774,502 to 4,777,126
_4314
Pf1N1B4_4315 - hypothetical protein, at 4,777,170 to 4,777,451
_4315
Pf1N1B4_4316 - Ribosomal protein S3AE, at 4,777,664 to 4,777,999
_4316
Position (kb)
4774
4775
4776
4777
4778 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4773.571 kb on + strand at 4774.039 kb on + strand, within Pf1N1B4_4313 at 4774.039 kb on + strand at 4774.039 kb on + strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.132 kb on - strand at 4774.132 kb on - strand, within Pf1N1B4_4313 at 4774.182 kb on + strand, within Pf1N1B4_4313 at 4774.182 kb on + strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.231 kb on + strand at 4774.231 kb on + strand at 4774.231 kb on + strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.281 kb on + strand at 4774.293 kb on + strand at 4774.293 kb on + strand at 4774.360 kb on + strand at 4774.361 kb on - strand at 4774.362 kb on + strand at 4774.362 kb on + strand at 4774.363 kb on - strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.463 kb on - strand at 4774.523 kb on + strand at 4774.649 kb on + strand at 4775.016 kb on - strand, within Pf1N1B4_4314 at 4775.237 kb on + strand, within Pf1N1B4_4314 at 4775.238 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.481 kb on - strand, within Pf1N1B4_4314 at 4775.483 kb on + strand, within Pf1N1B4_4314 at 4775.483 kb on + strand, within Pf1N1B4_4314 at 4775.483 kb on + strand, within Pf1N1B4_4314 at 4775.484 kb on - strand, within Pf1N1B4_4314 at 4775.484 kb on - strand at 4775.484 kb on - strand at 4775.484 kb on - strand, within Pf1N1B4_4314 at 4775.565 kb on + strand, within Pf1N1B4_4314 at 4775.590 kb on - strand, within Pf1N1B4_4314 at 4775.693 kb on + strand, within Pf1N1B4_4314 at 4775.693 kb on + strand, within Pf1N1B4_4314 at 4775.693 kb on + strand, within Pf1N1B4_4314 at 4775.694 kb on - strand, within Pf1N1B4_4314 at 4775.694 kb on - strand, within Pf1N1B4_4314 at 4775.694 kb on - strand, within Pf1N1B4_4314 at 4775.897 kb on + strand, within Pf1N1B4_4314 at 4775.898 kb on - strand, within Pf1N1B4_4314 at 4776.323 kb on + strand, within Pf1N1B4_4314 at 4776.324 kb on - strand, within Pf1N1B4_4314 at 4776.324 kb on - strand, within Pf1N1B4_4314 at 4776.326 kb on + strand, within Pf1N1B4_4314 at 4776.326 kb on + strand, within Pf1N1B4_4314 at 4776.349 kb on - strand, within Pf1N1B4_4314 at 4776.869 kb on - strand at 4776.869 kb on - strand at 4776.910 kb on + strand at 4776.911 kb on - strand at 4776.911 kb on - strand at 4777.124 kb on + strand at 4777.172 kb on - strand at 4777.447 kb on - strand at 4777.580 kb on - strand at 4777.641 kb on + strand at 4777.872 kb on + strand, within Pf1N1B4_4316 at 4777.873 kb on - strand, within Pf1N1B4_4316 at 4778.079 kb on - strand at 4778.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,773,571 + -0.9 4,774,039 + Pf1N1B4_4313 0.68 +0.7 4,774,039 + -0.9 4,774,039 + Pf1N1B4_4313 0.68 +1.5 4,774,040 - Pf1N1B4_4313 0.68 +0.3 4,774,040 - Pf1N1B4_4313 0.68 -1.3 4,774,040 - Pf1N1B4_4313 0.68 -0.6 4,774,040 - +1.5 4,774,131 + Pf1N1B4_4313 0.81 +0.7 4,774,131 + Pf1N1B4_4313 0.81 +0.6 4,774,131 + Pf1N1B4_4313 0.81 -1.6 4,774,132 - +0.7 4,774,132 - Pf1N1B4_4313 0.81 -0.6 4,774,182 + Pf1N1B4_4313 0.88 -1.3 4,774,182 + Pf1N1B4_4313 0.88 +0.5 4,774,183 - Pf1N1B4_4313 0.88 -1.6 4,774,183 - Pf1N1B4_4313 0.88 -2.1 4,774,183 - Pf1N1B4_4313 0.88 +2.3 4,774,231 + -0.6 4,774,231 + +0.2 4,774,231 + -0.9 4,774,232 - -0.3 4,774,232 - -0.9 4,774,232 - -1.3 4,774,232 - -0.9 4,774,281 + +1.1 4,774,293 + -0.1 4,774,293 + -0.3 4,774,360 + +0.0 4,774,361 - +0.0 4,774,362 + -2.6 4,774,362 + +0.6 4,774,363 - +0.7 4,774,462 + +1.3 4,774,462 + -1.3 4,774,462 + -0.9 4,774,462 + +0.7 4,774,462 + +1.1 4,774,463 - -0.1 4,774,523 + +0.0 4,774,649 + -1.1 4,775,016 - Pf1N1B4_4314 0.20 -0.2 4,775,237 + Pf1N1B4_4314 0.28 -0.6 4,775,238 - Pf1N1B4_4314 0.28 -1.1 4,775,481 - Pf1N1B4_4314 0.37 +0.8 4,775,481 - Pf1N1B4_4314 0.37 +1.2 4,775,481 - Pf1N1B4_4314 0.37 -1.6 4,775,481 - Pf1N1B4_4314 0.37 -0.2 4,775,483 + Pf1N1B4_4314 0.37 +0.6 4,775,483 + Pf1N1B4_4314 0.37 +0.2 4,775,483 + Pf1N1B4_4314 0.37 -1.3 4,775,484 - Pf1N1B4_4314 0.37 -2.5 4,775,484 - +0.7 4,775,484 - -0.3 4,775,484 - Pf1N1B4_4314 0.37 -2.3 4,775,565 + Pf1N1B4_4314 0.40 +0.7 4,775,590 - Pf1N1B4_4314 0.41 +0.4 4,775,693 + Pf1N1B4_4314 0.45 +0.7 4,775,693 + Pf1N1B4_4314 0.45 +0.1 4,775,693 + Pf1N1B4_4314 0.45 +1.7 4,775,694 - Pf1N1B4_4314 0.45 -0.3 4,775,694 - Pf1N1B4_4314 0.45 +0.7 4,775,694 - Pf1N1B4_4314 0.45 -0.5 4,775,897 + Pf1N1B4_4314 0.53 +0.7 4,775,898 - Pf1N1B4_4314 0.53 -0.4 4,776,323 + Pf1N1B4_4314 0.69 -0.5 4,776,324 - Pf1N1B4_4314 0.69 -0.1 4,776,324 - Pf1N1B4_4314 0.69 +1.1 4,776,326 + Pf1N1B4_4314 0.69 +1.5 4,776,326 + Pf1N1B4_4314 0.69 +0.7 4,776,349 - Pf1N1B4_4314 0.70 +0.1 4,776,869 - -0.3 4,776,869 - +3.3 4,776,910 + +2.0 4,776,911 - -0.6 4,776,911 - -0.3 4,777,124 + -1.3 4,777,172 - +1.9 4,777,447 - +0.7 4,777,580 - -2.0 4,777,641 + -1.6 4,777,872 + Pf1N1B4_4316 0.62 -1.2 4,777,873 - Pf1N1B4_4316 0.62 -0.3 4,778,079 - -0.7 4,778,087 - -0.8
Or see this region's nucleotide sequence