Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4195 and Pf1N1B4_4196 overlap by 37 nucleotides Pf1N1B4_4196 and Pf1N1B4_4197 overlap by 2 nucleotides Pf1N1B4_4197 and Pf1N1B4_4198 are separated by 282 nucleotides Pf1N1B4_4198 and Pf1N1B4_4199 are separated by 24 nucleotides
Pf1N1B4_4195 - Transcriptional regulator, TetR family, at 4,647,933 to 4,648,505
_4195
Pf1N1B4_4196 - hypothetical protein, at 4,648,469 to 4,648,630
_4196
Pf1N1B4_4197 - Glucose ABC transport system, periplasmic sugar-binding protein, at 4,648,629 to 4,648,754
_4197
Pf1N1B4_4198 - 4-hydroxybenzoate transporter, at 4,649,037 to 4,650,374
_4198
Pf1N1B4_4199 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 4,650,399 to 4,651,661
_4199
Position (kb)
4649
4650
4651 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.329 kb on + strand, within Pf1N1B4_4195 at 4648.800 kb on + strand at 4648.874 kb on - strand at 4648.952 kb on + strand at 4648.952 kb on + strand at 4649.015 kb on - strand at 4649.022 kb on + strand at 4649.022 kb on + strand at 4649.117 kb on - strand at 4649.128 kb on + strand at 4649.692 kb on + strand, within Pf1N1B4_4198 at 4649.826 kb on + strand, within Pf1N1B4_4198 at 4649.826 kb on + strand, within Pf1N1B4_4198 at 4649.850 kb on + strand, within Pf1N1B4_4198 at 4649.859 kb on - strand, within Pf1N1B4_4198 at 4650.558 kb on + strand, within Pf1N1B4_4199 at 4650.559 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,648,185 + +0.7 4,648,185 + Pf1N1B4_4195 0.44 -1.1 4,648,185 + Pf1N1B4_4195 0.44 -0.3 4,648,185 + Pf1N1B4_4195 0.44 -0.3 4,648,185 + Pf1N1B4_4195 0.44 +0.0 4,648,185 + Pf1N1B4_4195 0.44 -0.3 4,648,185 + Pf1N1B4_4195 0.44 +0.8 4,648,185 + -0.3 4,648,185 + Pf1N1B4_4195 0.44 -0.9 4,648,185 + -0.6 4,648,186 - Pf1N1B4_4195 0.44 -0.3 4,648,186 - Pf1N1B4_4195 0.44 -0.3 4,648,186 - Pf1N1B4_4195 0.44 -1.3 4,648,186 - +0.1 4,648,186 - Pf1N1B4_4195 0.44 -1.6 4,648,186 - Pf1N1B4_4195 0.44 +3.5 4,648,329 + Pf1N1B4_4195 0.69 -0.6 4,648,800 + +0.5 4,648,874 - -0.4 4,648,952 + -0.9 4,648,952 + +1.7 4,649,015 - +1.0 4,649,022 + -0.5 4,649,022 + +0.4 4,649,117 - -1.3 4,649,128 + +0.7 4,649,692 + Pf1N1B4_4198 0.49 +0.7 4,649,826 + Pf1N1B4_4198 0.59 +0.7 4,649,826 + Pf1N1B4_4198 0.59 +0.7 4,649,850 + Pf1N1B4_4198 0.61 +1.5 4,649,859 - Pf1N1B4_4198 0.61 +0.1 4,650,558 + Pf1N1B4_4199 0.13 -0.9 4,650,559 - Pf1N1B4_4199 0.13 +0.2 4,651,183 - Pf1N1B4_4199 0.62 -0.2 4,651,183 - Pf1N1B4_4199 0.62 -0.9 4,651,185 + Pf1N1B4_4199 0.62 -3.0 4,651,185 + Pf1N1B4_4199 0.62 -1.6 4,651,185 + Pf1N1B4_4199 0.62 -0.4
Or see this region's nucleotide sequence