Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_390

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_388 and Pf1N1B4_389 overlap by 4 nucleotidesPf1N1B4_389 and Pf1N1B4_390 are separated by 9 nucleotidesPf1N1B4_390 and Pf1N1B4_391 are separated by 106 nucleotides Pf1N1B4_388 - OmpA family protein, at 456,816 to 457,628 _388 Pf1N1B4_389 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 457,625 to 457,981 _389 Pf1N1B4_390 - Extracellular solute-binding protein, family 3, at 457,991 to 458,827 _390 Pf1N1B4_391 - Electron transfer flavoprotein, alpha subunit, at 458,934 to 459,863 _391 Position (kb) 457 458 459Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 457.048 kb on + strand, within Pf1N1B4_388at 457.049 kb on - strand, within Pf1N1B4_388at 457.049 kb on - strand, within Pf1N1B4_388at 457.366 kb on + strand, within Pf1N1B4_388at 457.367 kb on - strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.382 kb on - strand, within Pf1N1B4_388at 457.387 kb on + strand, within Pf1N1B4_388at 457.388 kb on - strand, within Pf1N1B4_388at 457.388 kb on - strand, within Pf1N1B4_388at 457.418 kb on - strand, within Pf1N1B4_388at 457.418 kb on - strand, within Pf1N1B4_388at 457.571 kb on - strandat 457.670 kb on - strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.975 kb on + strandat 457.975 kb on + strandat 457.976 kb on - strandat 457.976 kb on - strandat 457.982 kb on + strandat 457.983 kb on - strandat 457.992 kb on + strandat 458.113 kb on + strand, within Pf1N1B4_390at 458.155 kb on + strand, within Pf1N1B4_390at 458.257 kb on - strand, within Pf1N1B4_390at 458.375 kb on + strand, within Pf1N1B4_390at 458.375 kb on + strand, within Pf1N1B4_390at 458.433 kb on + strand, within Pf1N1B4_390at 458.441 kb on + strand, within Pf1N1B4_390at 458.469 kb on + strand, within Pf1N1B4_390at 458.499 kb on + strand, within Pf1N1B4_390at 458.499 kb on + strandat 458.499 kb on + strand, within Pf1N1B4_390at 458.499 kb on + strand, within Pf1N1B4_390at 458.500 kb on - strand, within Pf1N1B4_390at 458.500 kb on - strand, within Pf1N1B4_390at 458.601 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.673 kb on + strand, within Pf1N1B4_390at 458.674 kb on - strand, within Pf1N1B4_390at 458.674 kb on - strand, within Pf1N1B4_390at 458.704 kb on - strand, within Pf1N1B4_390at 458.795 kb on + strandat 458.840 kb on + strandat 458.840 kb on + strandat 458.841 kb on - strandat 458.841 kb on - strandat 458.850 kb on + strandat 458.872 kb on + strandat 458.872 kb on + strandat 458.915 kb on + strandat 458.915 kb on + strandat 458.915 kb on + strandat 458.916 kb on - strandat 458.916 kb on - strandat 458.916 kb on - strandat 458.916 kb on - strandat 458.916 kb on - strandat 458.916 kb on - strandat 458.916 kb on - strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.917 kb on + strandat 458.918 kb on - strandat 458.918 kb on - strandat 458.918 kb on - strandat 458.918 kb on - strandat 458.935 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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457,048 + Pf1N1B4_388 0.29 +1.9
457,049 - Pf1N1B4_388 0.29 +1.4
457,049 - Pf1N1B4_388 0.29 +2.5
457,366 + Pf1N1B4_388 0.68 +3.2
457,367 - Pf1N1B4_388 0.68 +0.8
457,381 + Pf1N1B4_388 0.69 +2.7
457,381 + Pf1N1B4_388 0.69 -0.5
457,381 + Pf1N1B4_388 0.69 +4.5
457,381 + Pf1N1B4_388 0.69 +3.8
457,381 + Pf1N1B4_388 0.69 +2.5
457,382 - Pf1N1B4_388 0.70 +2.8
457,387 + Pf1N1B4_388 0.70 +2.5
457,388 - Pf1N1B4_388 0.70 +4.2
457,388 - Pf1N1B4_388 0.70 +2.2
457,418 - Pf1N1B4_388 0.74 +2.4
457,418 - Pf1N1B4_388 0.74 +2.3
457,571 - +0.6
457,670 - Pf1N1B4_389 0.13 +2.0
457,898 + Pf1N1B4_389 0.76 +1.6
457,898 + Pf1N1B4_389 0.76 +2.6
457,898 + Pf1N1B4_389 0.76 +2.8
457,898 + Pf1N1B4_389 0.76 +1.5
457,898 + Pf1N1B4_389 0.76 +2.0
457,898 + Pf1N1B4_389 0.76 +2.8
457,898 + Pf1N1B4_389 0.76 +3.2
457,898 + Pf1N1B4_389 0.76 +2.1
457,898 + Pf1N1B4_389 0.76 +3.2
457,898 + Pf1N1B4_389 0.76 +1.8
457,898 + Pf1N1B4_389 0.76 +2.0
457,898 + Pf1N1B4_389 0.76 +2.2
457,898 + Pf1N1B4_389 0.76 +2.9
457,898 + Pf1N1B4_389 0.76 -0.8
457,898 + Pf1N1B4_389 0.76 +1.8
457,899 - Pf1N1B4_389 0.77 +2.0
457,899 - Pf1N1B4_389 0.77 +2.6
457,899 - Pf1N1B4_389 0.77 +4.0
457,899 - Pf1N1B4_389 0.77 +2.4
457,899 - Pf1N1B4_389 0.77 +1.8
457,899 - Pf1N1B4_389 0.77 +2.0
457,899 - Pf1N1B4_389 0.77 +0.1
457,975 + +2.7
457,975 + +2.4
457,976 - +2.0
457,976 - +2.0
457,982 + +3.0
457,983 - +1.9
457,992 + +0.5
458,113 + Pf1N1B4_390 0.15 +1.3
458,155 + Pf1N1B4_390 0.20 +2.0
458,257 - Pf1N1B4_390 0.32 -1.6
458,375 + Pf1N1B4_390 0.46 +0.0
458,375 + Pf1N1B4_390 0.46 +0.1
458,433 + Pf1N1B4_390 0.53 -0.6
458,441 + Pf1N1B4_390 0.54 -0.1
458,469 + Pf1N1B4_390 0.57 +1.4
458,499 + Pf1N1B4_390 0.61 -1.4
458,499 + +0.8
458,499 + Pf1N1B4_390 0.61 -1.2
458,499 + Pf1N1B4_390 0.61 +1.3
458,500 - Pf1N1B4_390 0.61 +0.7
458,500 - Pf1N1B4_390 0.61 +0.6
458,601 + Pf1N1B4_390 0.73 -0.7
458,673 + Pf1N1B4_390 0.81 -1.7
458,673 + Pf1N1B4_390 0.81 -0.5
458,673 + Pf1N1B4_390 0.81 -1.0
458,673 + Pf1N1B4_390 0.81 +0.3
458,673 + Pf1N1B4_390 0.81 -1.4
458,673 + Pf1N1B4_390 0.81 -1.5
458,673 + Pf1N1B4_390 0.81 -0.4
458,673 + Pf1N1B4_390 0.81 -1.1
458,673 + Pf1N1B4_390 0.81 +1.3
458,674 - Pf1N1B4_390 0.82 +0.8
458,674 - Pf1N1B4_390 0.82 +0.8
458,704 - Pf1N1B4_390 0.85 -1.2
458,795 + -2.0
458,840 + -0.2
458,840 + -1.7
458,841 - +1.6
458,841 - +0.1
458,850 + +2.2
458,872 + +1.8
458,872 + -1.2
458,915 + -1.0
458,915 + +0.6
458,915 + +0.5
458,916 - -0.2
458,916 - -0.4
458,916 - +0.6
458,916 - -0.7
458,916 - +0.4
458,916 - +0.5
458,916 - +0.6
458,917 + +0.6
458,917 + +0.5
458,917 + -0.7
458,917 + +0.6
458,917 + -0.7
458,917 + +0.5
458,917 + +0.3
458,917 + +1.4
458,917 + +0.8
458,917 + +0.7
458,917 + -0.2
458,917 + +1.0
458,918 - -2.0
458,918 - +0.1
458,918 - +3.2
458,918 - +1.6
458,935 + -0.2

Or see this region's nucleotide sequence