Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3624 and Pf1N1B4_3625 are separated by 233 nucleotides Pf1N1B4_3625 and Pf1N1B4_3626 overlap by 4 nucleotides Pf1N1B4_3626 and Pf1N1B4_3627 are separated by 156 nucleotides
Pf1N1B4_3624 - Flagellar biosynthesis protein FlhF, at 4,032,413 to 4,033,336
_3624
Pf1N1B4_3625 - Flagellar synthesis regulator FleN, at 4,033,570 to 4,034,283
_3625
Pf1N1B4_3626 - RNA polymerase sigma factor for flagellar operon, at 4,034,280 to 4,035,020
_3626
Pf1N1B4_3627 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 4,035,177 to 4,035,560
_3627
Position (kb)
4033
4034
4035 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4032.744 kb on - strand, within Pf1N1B4_3624 at 4032.755 kb on + strand, within Pf1N1B4_3624 at 4033.047 kb on + strand, within Pf1N1B4_3624 at 4033.048 kb on - strand, within Pf1N1B4_3624 at 4033.048 kb on - strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.068 kb on - strand, within Pf1N1B4_3624 at 4033.068 kb on - strand, within Pf1N1B4_3624 at 4033.102 kb on + strand, within Pf1N1B4_3624 at 4033.103 kb on - strand, within Pf1N1B4_3624 at 4033.172 kb on + strand, within Pf1N1B4_3624 at 4033.173 kb on - strand, within Pf1N1B4_3624 at 4033.251 kb on + strand at 4033.251 kb on + strand at 4033.251 kb on + strand at 4033.307 kb on + strand at 4033.307 kb on + strand at 4033.340 kb on - strand at 4033.340 kb on - strand at 4033.342 kb on + strand at 4033.342 kb on + strand at 4033.342 kb on + strand at 4033.343 kb on - strand at 4033.358 kb on + strand at 4033.358 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.363 kb on - strand at 4033.363 kb on - strand at 4033.363 kb on - strand at 4033.428 kb on - strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.432 kb on - strand at 4033.432 kb on - strand at 4033.432 kb on - strand at 4033.449 kb on + strand at 4033.449 kb on + strand at 4033.679 kb on + strand, within Pf1N1B4_3625 at 4033.679 kb on + strand, within Pf1N1B4_3625 at 4033.679 kb on + strand at 4033.680 kb on - strand at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4034.066 kb on + strand, within Pf1N1B4_3625 at 4034.067 kb on - strand at 4034.067 kb on - strand, within Pf1N1B4_3625 at 4034.071 kb on + strand, within Pf1N1B4_3625 at 4034.134 kb on + strand, within Pf1N1B4_3625 at 4034.196 kb on + strand, within Pf1N1B4_3625 at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.280 kb on - strand at 4034.296 kb on - strand at 4034.296 kb on - strand at 4034.296 kb on - strand at 4034.304 kb on + strand at 4034.305 kb on - strand at 4034.305 kb on - strand at 4034.376 kb on + strand, within Pf1N1B4_3626 at 4034.455 kb on + strand, within Pf1N1B4_3626 at 4034.455 kb on + strand, within Pf1N1B4_3626 at 4034.455 kb on + strand, within Pf1N1B4_3626 at 4034.455 kb on + strand, within Pf1N1B4_3626 at 4034.456 kb on - strand, within Pf1N1B4_3626 at 4034.456 kb on - strand, within Pf1N1B4_3626 at 4034.456 kb on - strand, within Pf1N1B4_3626 at 4034.500 kb on - strand, within Pf1N1B4_3626 at 4034.618 kb on + strand, within Pf1N1B4_3626 at 4034.682 kb on + strand, within Pf1N1B4_3626 at 4034.889 kb on + strand, within Pf1N1B4_3626 at 4034.890 kb on - strand, within Pf1N1B4_3626 at 4034.892 kb on + strand, within Pf1N1B4_3626 at 4034.962 kb on + strand at 4035.088 kb on + strand at 4035.088 kb on + strand at 4035.088 kb on + strand at 4035.089 kb on - strand at 4035.095 kb on + strand at 4035.095 kb on + strand at 4035.095 kb on + strand at 4035.095 kb on + strand at 4035.095 kb on + strand at 4035.188 kb on + strand at 4035.188 kb on + strand at 4035.189 kb on - strand at 4035.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 4,032,744 - Pf1N1B4_3624 0.36 -0.5 4,032,755 + Pf1N1B4_3624 0.37 -0.4 4,033,047 + Pf1N1B4_3624 0.69 +0.2 4,033,048 - Pf1N1B4_3624 0.69 -0.1 4,033,048 - Pf1N1B4_3624 0.69 +1.6 4,033,067 + Pf1N1B4_3624 0.71 -1.1 4,033,067 + Pf1N1B4_3624 0.71 -0.0 4,033,067 + Pf1N1B4_3624 0.71 -0.7 4,033,067 + Pf1N1B4_3624 0.71 -0.9 4,033,068 - Pf1N1B4_3624 0.71 +0.9 4,033,068 - Pf1N1B4_3624 0.71 +0.6 4,033,102 + Pf1N1B4_3624 0.75 +2.2 4,033,103 - Pf1N1B4_3624 0.75 -0.3 4,033,172 + Pf1N1B4_3624 0.82 -2.1 4,033,173 - Pf1N1B4_3624 0.82 -0.7 4,033,251 + +2.3 4,033,251 + +1.2 4,033,251 + -0.1 4,033,307 + -1.7 4,033,307 + -0.0 4,033,340 - +0.7 4,033,340 - -2.1 4,033,342 + -0.5 4,033,342 + +0.4 4,033,342 + -0.1 4,033,343 - -0.1 4,033,358 + -2.3 4,033,358 + -0.1 4,033,362 + +1.5 4,033,362 + +0.4 4,033,362 + -0.1 4,033,362 + +0.5 4,033,362 + +0.9 4,033,362 + +2.5 4,033,362 + +1.1 4,033,362 + +0.3 4,033,363 - -0.1 4,033,363 - -0.1 4,033,363 - -0.7 4,033,428 - +0.5 4,033,431 + -1.1 4,033,431 + -0.1 4,033,431 + +0.2 4,033,431 + +1.2 4,033,432 - -0.1 4,033,432 - -1.1 4,033,432 - +0.5 4,033,449 + -1.1 4,033,449 + +2.5 4,033,679 + Pf1N1B4_3625 0.15 -1.9 4,033,679 + Pf1N1B4_3625 0.15 +0.3 4,033,679 + -0.1 4,033,680 - -0.7 4,033,811 + Pf1N1B4_3625 0.34 -2.0 4,033,811 + Pf1N1B4_3625 0.34 -1.3 4,033,811 + Pf1N1B4_3625 0.34 -0.7 4,034,066 + Pf1N1B4_3625 0.69 -1.0 4,034,067 - -0.1 4,034,067 - Pf1N1B4_3625 0.70 +0.1 4,034,071 + Pf1N1B4_3625 0.70 +0.3 4,034,134 + Pf1N1B4_3625 0.79 +0.3 4,034,196 + Pf1N1B4_3625 0.88 -0.5 4,034,279 + -0.7 4,034,279 + +2.6 4,034,279 + +0.1 4,034,279 + +2.5 4,034,279 + -0.4 4,034,280 - -1.7 4,034,296 - +2.5 4,034,296 - +0.1 4,034,296 - +2.9 4,034,304 + +1.9 4,034,305 - -2.1 4,034,305 - +2.1 4,034,376 + Pf1N1B4_3626 0.13 +0.9 4,034,455 + Pf1N1B4_3626 0.24 +1.0 4,034,455 + Pf1N1B4_3626 0.24 +1.5 4,034,455 + Pf1N1B4_3626 0.24 +0.9 4,034,455 + Pf1N1B4_3626 0.24 +0.7 4,034,456 - Pf1N1B4_3626 0.24 +0.9 4,034,456 - Pf1N1B4_3626 0.24 +0.5 4,034,456 - Pf1N1B4_3626 0.24 +1.2 4,034,500 - Pf1N1B4_3626 0.30 +0.0 4,034,618 + Pf1N1B4_3626 0.46 +0.1 4,034,682 + Pf1N1B4_3626 0.54 +1.1 4,034,889 + Pf1N1B4_3626 0.82 -0.6 4,034,890 - Pf1N1B4_3626 0.82 +0.4 4,034,892 + Pf1N1B4_3626 0.83 -0.5 4,034,962 + +1.6 4,035,088 + +3.6 4,035,088 + -0.7 4,035,088 + -0.1 4,035,089 - -0.1 4,035,095 + +0.7 4,035,095 + +0.1 4,035,095 + +1.5 4,035,095 + -0.7 4,035,095 + +0.3 4,035,188 + -2.3 4,035,188 + -0.1 4,035,189 - -1.1 4,035,235 + +0.9
Or see this region's nucleotide sequence