Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3491 and Pf1N1B4_3492 are separated by 131 nucleotides Pf1N1B4_3492 and Pf1N1B4_3493 are separated by 115 nucleotides Pf1N1B4_3493 and Pf1N1B4_3494 are separated by 1 nucleotides
Pf1N1B4_3491 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 3,898,839 to 3,899,663
_3491
Pf1N1B4_3492 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 3,899,795 to 3,900,469
_3492
Pf1N1B4_3493 - FIG00956341: hypothetical protein, at 3,900,585 to 3,901,010
_3493
Pf1N1B4_3494 - Flavoprotein WrbA, at 3,901,012 to 3,901,608
_3494
Position (kb)
3899
3900
3901 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3898.850 kb on + strand at 3898.850 kb on + strand at 3898.974 kb on + strand at 3898.974 kb on + strand, within Pf1N1B4_3491 at 3898.974 kb on + strand, within Pf1N1B4_3491 at 3898.975 kb on - strand, within Pf1N1B4_3491 at 3898.975 kb on - strand, within Pf1N1B4_3491 at 3898.975 kb on - strand at 3898.975 kb on - strand, within Pf1N1B4_3491 at 3899.091 kb on + strand, within Pf1N1B4_3491 at 3899.091 kb on + strand, within Pf1N1B4_3491 at 3899.139 kb on + strand, within Pf1N1B4_3491 at 3899.139 kb on + strand, within Pf1N1B4_3491 at 3899.140 kb on - strand, within Pf1N1B4_3491 at 3899.159 kb on + strand, within Pf1N1B4_3491 at 3899.217 kb on + strand, within Pf1N1B4_3491 at 3899.654 kb on - strand at 3899.654 kb on - strand at 3899.654 kb on - strand at 3899.769 kb on + strand at 3899.770 kb on - strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.805 kb on - strand at 3899.805 kb on - strand at 3899.805 kb on - strand at 3899.823 kb on - strand at 3900.215 kb on + strand at 3900.215 kb on + strand, within Pf1N1B4_3492 at 3900.215 kb on + strand, within Pf1N1B4_3492 at 3900.216 kb on - strand, within Pf1N1B4_3492 at 3900.216 kb on - strand, within Pf1N1B4_3492 at 3900.216 kb on - strand, within Pf1N1B4_3492 at 3900.302 kb on + strand, within Pf1N1B4_3492 at 3900.302 kb on + strand, within Pf1N1B4_3492 at 3900.303 kb on - strand, within Pf1N1B4_3492 at 3900.355 kb on + strand, within Pf1N1B4_3492 at 3900.356 kb on - strand, within Pf1N1B4_3492 at 3900.512 kb on - strand at 3900.512 kb on - strand at 3900.581 kb on + strand at 3900.649 kb on + strand, within Pf1N1B4_3493 at 3900.649 kb on + strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.752 kb on - strand, within Pf1N1B4_3493 at 3900.893 kb on - strand, within Pf1N1B4_3493 at 3900.893 kb on - strand, within Pf1N1B4_3493 at 3900.893 kb on - strand, within Pf1N1B4_3493 at 3900.895 kb on + strand at 3900.895 kb on + strand, within Pf1N1B4_3493 at 3900.895 kb on + strand, within Pf1N1B4_3493 at 3900.895 kb on + strand, within Pf1N1B4_3493 at 3900.896 kb on - strand, within Pf1N1B4_3493 at 3900.896 kb on - strand, within Pf1N1B4_3493 at 3900.896 kb on - strand, within Pf1N1B4_3493 at 3900.896 kb on - strand, within Pf1N1B4_3493 at 3901.130 kb on + strand, within Pf1N1B4_3494 at 3901.176 kb on - strand, within Pf1N1B4_3494 at 3901.340 kb on + strand, within Pf1N1B4_3494 at 3901.341 kb on - strand, within Pf1N1B4_3494
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 3,898,850 + -2.1 3,898,850 + +0.4 3,898,974 + -0.4 3,898,974 + Pf1N1B4_3491 0.16 +1.2 3,898,974 + Pf1N1B4_3491 0.16 -1.9 3,898,975 - Pf1N1B4_3491 0.16 -0.1 3,898,975 - Pf1N1B4_3491 0.16 -0.6 3,898,975 - +1.5 3,898,975 - Pf1N1B4_3491 0.16 -0.4 3,899,091 + Pf1N1B4_3491 0.31 +0.3 3,899,091 + Pf1N1B4_3491 0.31 +0.5 3,899,139 + Pf1N1B4_3491 0.36 +1.2 3,899,139 + Pf1N1B4_3491 0.36 -1.4 3,899,140 - Pf1N1B4_3491 0.36 +0.3 3,899,159 + Pf1N1B4_3491 0.39 +0.9 3,899,217 + Pf1N1B4_3491 0.46 -0.3 3,899,654 - -0.5 3,899,654 - +1.0 3,899,654 - +0.9 3,899,769 + -1.7 3,899,770 - +0.6 3,899,777 + +1.9 3,899,777 + +0.4 3,899,777 + -0.6 3,899,777 + -1.1 3,899,777 + +1.5 3,899,777 + +0.4 3,899,777 + -0.1 3,899,777 + -1.1 3,899,778 - -0.1 3,899,778 - -0.7 3,899,778 - -0.7 3,899,778 - -1.1 3,899,778 - +0.4 3,899,778 - -1.1 3,899,778 - -0.5 3,899,778 - -1.9 3,899,778 - +0.2 3,899,805 - +0.1 3,899,805 - +0.2 3,899,805 - +0.9 3,899,823 - -2.2 3,900,215 + +2.5 3,900,215 + Pf1N1B4_3492 0.62 -1.1 3,900,215 + Pf1N1B4_3492 0.62 +0.9 3,900,216 - Pf1N1B4_3492 0.62 +0.7 3,900,216 - Pf1N1B4_3492 0.62 -0.4 3,900,216 - Pf1N1B4_3492 0.62 -0.7 3,900,302 + Pf1N1B4_3492 0.75 -0.2 3,900,302 + Pf1N1B4_3492 0.75 -0.5 3,900,303 - Pf1N1B4_3492 0.75 -0.7 3,900,355 + Pf1N1B4_3492 0.83 -0.1 3,900,356 - Pf1N1B4_3492 0.83 -0.4 3,900,512 - -0.9 3,900,512 - +0.3 3,900,581 + -1.5 3,900,649 + Pf1N1B4_3493 0.15 -1.5 3,900,649 + Pf1N1B4_3493 0.15 +0.7 3,900,650 - Pf1N1B4_3493 0.15 -1.1 3,900,650 - Pf1N1B4_3493 0.15 -0.8 3,900,650 - Pf1N1B4_3493 0.15 +0.6 3,900,650 - Pf1N1B4_3493 0.15 -1.5 3,900,650 - Pf1N1B4_3493 0.15 -0.7 3,900,650 - Pf1N1B4_3493 0.15 +1.2 3,900,650 - Pf1N1B4_3493 0.15 +1.5 3,900,650 - -0.1 3,900,650 - Pf1N1B4_3493 0.15 -0.1 3,900,650 - Pf1N1B4_3493 0.15 +0.9 3,900,650 - Pf1N1B4_3493 0.15 +1.5 3,900,752 - Pf1N1B4_3493 0.39 +0.1 3,900,893 - Pf1N1B4_3493 0.72 -0.5 3,900,893 - Pf1N1B4_3493 0.72 -1.4 3,900,893 - Pf1N1B4_3493 0.72 -0.3 3,900,895 + -0.7 3,900,895 + Pf1N1B4_3493 0.73 -0.0 3,900,895 + Pf1N1B4_3493 0.73 +0.9 3,900,895 + Pf1N1B4_3493 0.73 -1.4 3,900,896 - Pf1N1B4_3493 0.73 +0.2 3,900,896 - Pf1N1B4_3493 0.73 -1.3 3,900,896 - Pf1N1B4_3493 0.73 -0.1 3,900,896 - Pf1N1B4_3493 0.73 -1.7 3,901,130 + Pf1N1B4_3494 0.20 -1.0 3,901,176 - Pf1N1B4_3494 0.27 -0.0 3,901,340 + Pf1N1B4_3494 0.55 +0.2 3,901,341 - Pf1N1B4_3494 0.55 +0.4
Or see this region's nucleotide sequence