Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_349

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_348 and Pf1N1B4_349 are separated by 28 nucleotidesPf1N1B4_349 and Pf1N1B4_350 are separated by 12 nucleotides Pf1N1B4_348 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 411,880 to 414,087 _348 Pf1N1B4_349 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 414,116 to 415,228 _349 Pf1N1B4_350 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 415,241 to 416,335 _350 Position (kb) 414 415 416Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 413.214 kb on - strand, within Pf1N1B4_348at 413.214 kb on - strand, within Pf1N1B4_348at 413.214 kb on - strand, within Pf1N1B4_348at 413.282 kb on - strand, within Pf1N1B4_348at 413.344 kb on - strand, within Pf1N1B4_348at 413.344 kb on - strand, within Pf1N1B4_348at 413.662 kb on - strand, within Pf1N1B4_348at 413.662 kb on - strand, within Pf1N1B4_348at 413.662 kb on - strand, within Pf1N1B4_348at 413.662 kb on - strand, within Pf1N1B4_348at 413.662 kb on - strand, within Pf1N1B4_348at 413.951 kb on - strandat 413.951 kb on - strandat 414.087 kb on + strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.088 kb on - strandat 414.211 kb on - strandat 414.396 kb on + strand, within Pf1N1B4_349at 414.396 kb on + strand, within Pf1N1B4_349at 414.397 kb on - strand, within Pf1N1B4_349at 414.397 kb on - strand, within Pf1N1B4_349at 414.535 kb on - strand, within Pf1N1B4_349at 414.535 kb on - strand, within Pf1N1B4_349at 414.585 kb on + strand, within Pf1N1B4_349at 414.586 kb on - strand, within Pf1N1B4_349at 414.622 kb on - strand, within Pf1N1B4_349at 414.630 kb on + strand, within Pf1N1B4_349at 414.631 kb on - strand, within Pf1N1B4_349at 414.631 kb on - strand, within Pf1N1B4_349at 414.631 kb on - strand, within Pf1N1B4_349at 414.631 kb on - strand, within Pf1N1B4_349at 414.852 kb on + strand, within Pf1N1B4_349at 414.904 kb on - strand, within Pf1N1B4_349at 415.174 kb on - strandat 415.174 kb on - strandat 415.284 kb on - strandat 415.284 kb on - strandat 415.284 kb on - strandat 415.311 kb on - strandat 415.365 kb on + strand, within Pf1N1B4_350at 415.365 kb on + strand, within Pf1N1B4_350at 415.365 kb on + strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.376 kb on - strand, within Pf1N1B4_350at 415.376 kb on - strand, within Pf1N1B4_350at 415.376 kb on - strand, within Pf1N1B4_350at 415.755 kb on + strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strandat 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 416.043 kb on + strand, within Pf1N1B4_350at 416.226 kb on - strandat 416.226 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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413,214 - Pf1N1B4_348 0.60 -1.2
413,214 - Pf1N1B4_348 0.60 +0.4
413,214 - Pf1N1B4_348 0.60 -2.3
413,282 - Pf1N1B4_348 0.63 -1.0
413,344 - Pf1N1B4_348 0.66 -1.2
413,344 - Pf1N1B4_348 0.66 -1.2
413,662 - Pf1N1B4_348 0.81 -1.2
413,662 - Pf1N1B4_348 0.81 -1.2
413,662 - Pf1N1B4_348 0.81 +0.1
413,662 - Pf1N1B4_348 0.81 -0.2
413,662 - Pf1N1B4_348 0.81 +2.8
413,951 - +0.8
413,951 - +1.1
414,087 + +4.9
414,088 - -0.2
414,088 - +0.8
414,088 - -0.5
414,088 - +0.0
414,088 - +0.2
414,088 - +0.6
414,088 - +1.8
414,088 - -1.2
414,088 - +0.2
414,088 - -1.3
414,088 - -1.0
414,088 - +0.2
414,088 - -1.8
414,088 - +0.1
414,211 - -0.6
414,396 + Pf1N1B4_349 0.25 +1.4
414,396 + Pf1N1B4_349 0.25 +1.9
414,397 - Pf1N1B4_349 0.25 +0.9
414,397 - Pf1N1B4_349 0.25 -1.2
414,535 - Pf1N1B4_349 0.38 -0.8
414,535 - Pf1N1B4_349 0.38 -1.0
414,585 + Pf1N1B4_349 0.42 +3.0
414,586 - Pf1N1B4_349 0.42 +0.4
414,622 - Pf1N1B4_349 0.45 +0.2
414,630 + Pf1N1B4_349 0.46 +1.9
414,631 - Pf1N1B4_349 0.46 +0.8
414,631 - Pf1N1B4_349 0.46 +0.4
414,631 - Pf1N1B4_349 0.46 +0.4
414,631 - Pf1N1B4_349 0.46 -0.2
414,852 + Pf1N1B4_349 0.66 +1.8
414,904 - Pf1N1B4_349 0.71 -0.1
415,174 - -0.3
415,174 - +4.1
415,284 - +0.2
415,284 - +1.1
415,284 - +0.9
415,311 - -1.2
415,365 + Pf1N1B4_350 0.11 +2.1
415,365 + Pf1N1B4_350 0.11 +1.5
415,365 + Pf1N1B4_350 0.11 +1.5
415,366 - Pf1N1B4_350 0.11 +1.0
415,366 - Pf1N1B4_350 0.11 +1.4
415,366 - Pf1N1B4_350 0.11 +0.1
415,366 - Pf1N1B4_350 0.11 -2.5
415,366 - Pf1N1B4_350 0.11 +1.1
415,366 - Pf1N1B4_350 0.11 -0.8
415,366 - Pf1N1B4_350 0.11 -0.1
415,366 - Pf1N1B4_350 0.11 +0.6
415,366 - Pf1N1B4_350 0.11 +1.2
415,366 - Pf1N1B4_350 0.11 +0.4
415,366 - Pf1N1B4_350 0.11 -0.5
415,366 - Pf1N1B4_350 0.11 -0.7
415,366 - Pf1N1B4_350 0.11 -1.5
415,366 - Pf1N1B4_350 0.11 +0.4
415,366 - Pf1N1B4_350 0.11 -1.5
415,366 - Pf1N1B4_350 0.11 -1.2
415,366 - Pf1N1B4_350 0.11 -1.5
415,366 - Pf1N1B4_350 0.11 -0.2
415,366 - Pf1N1B4_350 0.11 -0.4
415,376 - Pf1N1B4_350 0.12 -3.3
415,376 - Pf1N1B4_350 0.12 +0.2
415,376 - Pf1N1B4_350 0.12 -1.5
415,755 + Pf1N1B4_350 0.47 +2.1
415,756 - Pf1N1B4_350 0.47 +0.2
415,756 - -0.2
415,756 - Pf1N1B4_350 0.47 -1.2
415,756 - Pf1N1B4_350 0.47 +0.8
415,756 - Pf1N1B4_350 0.47 -0.3
415,756 - Pf1N1B4_350 0.47 +0.2
415,756 - Pf1N1B4_350 0.47 -0.9
416,043 + Pf1N1B4_350 0.73 +2.0
416,226 - +0.8
416,226 - -1.2

Or see this region's nucleotide sequence