Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3484

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3482 and Pf1N1B4_3483 are separated by 192 nucleotidesPf1N1B4_3483 and Pf1N1B4_3484 overlap by 4 nucleotidesPf1N1B4_3484 and Pf1N1B4_3485 are separated by 486 nucleotidesPf1N1B4_3485 and Pf1N1B4_3486 are separated by 126 nucleotides Pf1N1B4_3482 - outer membrane protein H1, at 3,889,223 to 3,889,825 _3482 Pf1N1B4_3483 - Transcriptional regulatory protein PhoP, at 3,890,018 to 3,890,695 _3483 Pf1N1B4_3484 - Sensor protein PhoQ (EC 2.7.13.3), at 3,890,692 to 3,892,038 _3484 Pf1N1B4_3485 - hypothetical protein, at 3,892,525 to 3,892,884 _3485 Pf1N1B4_3486 - Transcriptional regulator, AraC family, at 3,893,011 to 3,894,024 _3486 Position (kb) 3890 3891 3892 3893Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 3889.712 kb on + strand, within Pf1N1B4_3482at 3889.799 kb on + strandat 3889.799 kb on + strandat 3889.799 kb on + strandat 3889.799 kb on + strandat 3889.799 kb on + strandat 3889.799 kb on + strandat 3889.799 kb on + strandat 3889.800 kb on - strandat 3889.814 kb on + strandat 3889.814 kb on + strandat 3889.814 kb on + strandat 3889.814 kb on + strandat 3889.814 kb on + strandat 3889.823 kb on + strandat 3889.823 kb on + strandat 3889.823 kb on + strandat 3889.824 kb on - strandat 3889.824 kb on - strandat 3889.835 kb on + strandat 3889.835 kb on + strandat 3889.836 kb on - strandat 3889.980 kb on + strandat 3889.980 kb on + strandat 3889.980 kb on + strandat 3890.017 kb on + strandat 3890.129 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.309 kb on + strand, within Pf1N1B4_3483at 3890.310 kb on - strand, within Pf1N1B4_3483at 3890.475 kb on - strand, within Pf1N1B4_3483at 3890.495 kb on + strand, within Pf1N1B4_3483at 3890.495 kb on + strand, within Pf1N1B4_3483at 3890.496 kb on - strand, within Pf1N1B4_3483at 3890.496 kb on - strand, within Pf1N1B4_3483at 3890.938 kb on + strand, within Pf1N1B4_3484at 3890.938 kb on + strand, within Pf1N1B4_3484at 3890.939 kb on - strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.944 kb on + strand, within Pf1N1B4_3484at 3890.945 kb on - strand, within Pf1N1B4_3484at 3891.004 kb on + strand, within Pf1N1B4_3484at 3891.004 kb on + strand, within Pf1N1B4_3484at 3891.079 kb on + strand, within Pf1N1B4_3484at 3891.079 kb on + strand, within Pf1N1B4_3484at 3891.079 kb on + strand, within Pf1N1B4_3484at 3891.709 kb on + strand, within Pf1N1B4_3484at 3891.709 kb on + strand, within Pf1N1B4_3484at 3891.709 kb on + strand, within Pf1N1B4_3484at 3891.709 kb on + strand, within Pf1N1B4_3484at 3891.709 kb on + strand, within Pf1N1B4_3484at 3891.964 kb on + strandat 3891.964 kb on + strandat 3892.163 kb on + strandat 3892.178 kb on - strandat 3892.194 kb on + strandat 3892.235 kb on + strandat 3892.277 kb on + strandat 3892.277 kb on + strandat 3892.277 kb on + strandat 3892.278 kb on - strandat 3892.371 kb on + strandat 3892.459 kb on + strandat 3892.474 kb on + strandat 3892.474 kb on + strandat 3892.474 kb on + strandat 3892.475 kb on + strandat 3892.475 kb on - strandat 3892.488 kb on + strandat 3892.537 kb on - strandat 3892.537 kb on - strandat 3892.627 kb on + strand, within Pf1N1B4_3485at 3892.627 kb on + strand, within Pf1N1B4_3485at 3892.630 kb on + strandat 3892.634 kb on + strandat 3892.634 kb on + strand, within Pf1N1B4_3485at 3892.682 kb on - strand, within Pf1N1B4_3485at 3892.682 kb on - strand, within Pf1N1B4_3485at 3892.682 kb on - strand, within Pf1N1B4_3485

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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3,889,712 + Pf1N1B4_3482 0.81 +2.1
3,889,799 + +1.1
3,889,799 + -0.2
3,889,799 + +0.9
3,889,799 + +2.8
3,889,799 + +0.1
3,889,799 + +1.0
3,889,799 + +0.1
3,889,800 - -1.4
3,889,814 + -1.1
3,889,814 + -0.5
3,889,814 + +3.2
3,889,814 + +1.7
3,889,814 + +1.7
3,889,823 + +3.9
3,889,823 + +1.9
3,889,823 + +2.2
3,889,824 - -3.0
3,889,824 - -1.7
3,889,835 + +3.7
3,889,835 + +3.5
3,889,836 - -3.0
3,889,980 + +3.6
3,889,980 + +2.7
3,889,980 + +3.7
3,890,017 + -1.4
3,890,129 + Pf1N1B4_3483 0.16 -1.4
3,890,309 + Pf1N1B4_3483 0.43 -2.4
3,890,309 + Pf1N1B4_3483 0.43 -1.1
3,890,309 + Pf1N1B4_3483 0.43 -0.1
3,890,309 + Pf1N1B4_3483 0.43 -2.4
3,890,309 + Pf1N1B4_3483 0.43 -2.3
3,890,309 + Pf1N1B4_3483 0.43 -2.1
3,890,309 + Pf1N1B4_3483 0.43 -1.7
3,890,310 - Pf1N1B4_3483 0.43 -2.9
3,890,475 - Pf1N1B4_3483 0.67 -2.4
3,890,495 + Pf1N1B4_3483 0.70 -1.9
3,890,495 + Pf1N1B4_3483 0.70 -1.4
3,890,496 - Pf1N1B4_3483 0.71 -2.5
3,890,496 - Pf1N1B4_3483 0.71 -3.4
3,890,938 + Pf1N1B4_3484 0.18 +0.4
3,890,938 + Pf1N1B4_3484 0.18 +2.4
3,890,939 - Pf1N1B4_3484 0.18 +1.4
3,890,944 + Pf1N1B4_3484 0.19 +3.7
3,890,944 + Pf1N1B4_3484 0.19 +3.4
3,890,944 + Pf1N1B4_3484 0.19 +5.7
3,890,944 + Pf1N1B4_3484 0.19 +3.0
3,890,944 + Pf1N1B4_3484 0.19 +3.5
3,890,944 + Pf1N1B4_3484 0.19 +0.1
3,890,944 + Pf1N1B4_3484 0.19 +0.2
3,890,944 + Pf1N1B4_3484 0.19 +0.7
3,890,944 + Pf1N1B4_3484 0.19 +3.8
3,890,945 - Pf1N1B4_3484 0.19 -0.7
3,891,004 + Pf1N1B4_3484 0.23 +1.9
3,891,004 + Pf1N1B4_3484 0.23 +3.7
3,891,079 + Pf1N1B4_3484 0.29 +3.3
3,891,079 + Pf1N1B4_3484 0.29 +2.3
3,891,079 + Pf1N1B4_3484 0.29 +1.7
3,891,709 + Pf1N1B4_3484 0.76 +2.5
3,891,709 + Pf1N1B4_3484 0.76 +5.0
3,891,709 + Pf1N1B4_3484 0.76 +3.5
3,891,709 + Pf1N1B4_3484 0.76 +2.9
3,891,709 + Pf1N1B4_3484 0.76 +3.0
3,891,964 + +1.6
3,891,964 + +3.7
3,892,163 + +0.4
3,892,178 - +0.9
3,892,194 + -0.1
3,892,235 + +0.7
3,892,277 + +0.9
3,892,277 + +0.8
3,892,277 + -0.6
3,892,278 - -2.4
3,892,371 + +0.3
3,892,459 + -1.1
3,892,474 + -1.4
3,892,474 + +1.7
3,892,474 + +0.7
3,892,475 + -0.4
3,892,475 - -0.7
3,892,488 + +0.6
3,892,537 - -0.7
3,892,537 - +0.9
3,892,627 + Pf1N1B4_3485 0.28 -0.4
3,892,627 + Pf1N1B4_3485 0.28 +2.3
3,892,630 + +1.9
3,892,634 + +2.2
3,892,634 + Pf1N1B4_3485 0.30 +0.3
3,892,682 - Pf1N1B4_3485 0.44 -1.9
3,892,682 - Pf1N1B4_3485 0.44 -1.4
3,892,682 - Pf1N1B4_3485 0.44 -0.1

Or see this region's nucleotide sequence