Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_340

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_339 and Pf1N1B4_340 overlap by 4 nucleotidesPf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotidesPf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotides Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116 _339 Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036 _340 Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983 _341 Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873 _342 Position (kb) 404 405 406Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 403.148 kb on + strandat 403.148 kb on + strandat 403.149 kb on - strandat 403.159 kb on + strandat 403.493 kb on + strand, within Pf1N1B4_339at 403.765 kb on + strand, within Pf1N1B4_339at 403.895 kb on + strand, within Pf1N1B4_339at 404.132 kb on - strandat 404.155 kb on - strandat 404.155 kb on - strandat 404.219 kb on - strand, within Pf1N1B4_340at 404.383 kb on - strand, within Pf1N1B4_340at 404.427 kb on - strand, within Pf1N1B4_340at 404.427 kb on - strand, within Pf1N1B4_340at 404.460 kb on - strand, within Pf1N1B4_340at 404.466 kb on - strand, within Pf1N1B4_340at 404.555 kb on + strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strandat 404.559 kb on + strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.588 kb on + strand, within Pf1N1B4_340at 404.589 kb on - strand, within Pf1N1B4_340at 404.589 kb on - strand, within Pf1N1B4_340at 404.589 kb on - strand, within Pf1N1B4_340at 404.669 kb on + strand, within Pf1N1B4_340at 404.669 kb on + strand, within Pf1N1B4_340at 404.921 kb on + strand, within Pf1N1B4_340at 404.921 kb on + strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.993 kb on + strandat 404.993 kb on + strandat 404.993 kb on + strandat 404.994 kb on - strandat 404.994 kb on - strandat 404.994 kb on - strandat 404.994 kb on - strandat 405.029 kb on - strandat 405.029 kb on - strandat 405.128 kb on - strandat 405.128 kb on - strandat 405.128 kb on - strandat 405.359 kb on - strandat 405.443 kb on - strand, within Pf1N1B4_341at 405.443 kb on - strand, within Pf1N1B4_341at 405.443 kb on - strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.449 kb on - strand, within Pf1N1B4_341at 405.476 kb on + strand, within Pf1N1B4_341at 405.477 kb on - strand, within Pf1N1B4_341at 405.574 kb on - strand, within Pf1N1B4_341at 405.635 kb on - strand, within Pf1N1B4_341at 405.635 kb on - strand, within Pf1N1B4_341at 405.726 kb on - strand, within Pf1N1B4_341at 405.740 kb on - strand, within Pf1N1B4_341at 405.815 kb on + strandat 405.815 kb on + strand, within Pf1N1B4_341at 405.816 kb on - strand, within Pf1N1B4_341at 405.966 kb on - strandat 405.985 kb on + strandat 405.985 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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403,148 + +0.8
403,148 + +0.6
403,149 - -1.0
403,159 + +1.0
403,493 + Pf1N1B4_339 0.35 +1.9
403,765 + Pf1N1B4_339 0.63 +1.0
403,895 + Pf1N1B4_339 0.77 +2.3
404,132 - -0.8
404,155 - -0.8
404,155 - -3.1
404,219 - Pf1N1B4_340 0.11 -2.0
404,383 - Pf1N1B4_340 0.29 -1.2
404,427 - Pf1N1B4_340 0.34 -2.3
404,427 - Pf1N1B4_340 0.34 -0.2
404,460 - Pf1N1B4_340 0.38 -1.2
404,466 - Pf1N1B4_340 0.38 -0.2
404,555 + Pf1N1B4_340 0.48 +0.1
404,556 - Pf1N1B4_340 0.48 -2.5
404,556 - Pf1N1B4_340 0.48 -0.3
404,556 - Pf1N1B4_340 0.48 -1.8
404,556 - Pf1N1B4_340 0.48 -1.2
404,556 - Pf1N1B4_340 0.48 -2.4
404,556 - Pf1N1B4_340 0.48 -1.5
404,556 - Pf1N1B4_340 0.48 -0.2
404,556 - +0.2
404,559 + Pf1N1B4_340 0.48 -1.8
404,560 - Pf1N1B4_340 0.48 -1.9
404,560 - Pf1N1B4_340 0.48 -2.2
404,560 - Pf1N1B4_340 0.48 -2.0
404,560 - Pf1N1B4_340 0.48 -2.6
404,560 - Pf1N1B4_340 0.48 -2.2
404,588 + Pf1N1B4_340 0.51 -1.5
404,589 - Pf1N1B4_340 0.52 -1.5
404,589 - Pf1N1B4_340 0.52 -1.2
404,589 - Pf1N1B4_340 0.52 -1.5
404,669 + Pf1N1B4_340 0.60 +1.1
404,669 + Pf1N1B4_340 0.60 -2.0
404,921 + Pf1N1B4_340 0.87 +1.2
404,921 + Pf1N1B4_340 0.87 -1.2
404,922 - Pf1N1B4_340 0.88 -1.2
404,922 - Pf1N1B4_340 0.88 -0.8
404,922 - Pf1N1B4_340 0.88 -1.2
404,922 - Pf1N1B4_340 0.88 -2.2
404,922 - Pf1N1B4_340 0.88 -1.4
404,922 - Pf1N1B4_340 0.88 -0.5
404,993 + -1.2
404,993 + -0.2
404,993 + -2.0
404,994 - +0.8
404,994 - -2.0
404,994 - -2.2
404,994 - -0.2
405,029 - -1.2
405,029 - +0.4
405,128 - +1.0
405,128 - -1.0
405,128 - -0.8
405,359 - -0.2
405,443 - Pf1N1B4_341 0.39 +1.4
405,443 - Pf1N1B4_341 0.39 -0.8
405,443 - Pf1N1B4_341 0.39 +0.4
405,448 + Pf1N1B4_341 0.40 +1.0
405,448 + Pf1N1B4_341 0.40 -0.5
405,448 + Pf1N1B4_341 0.40 +1.0
405,449 - Pf1N1B4_341 0.40 -0.6
405,476 + Pf1N1B4_341 0.43 -1.8
405,477 - Pf1N1B4_341 0.43 -0.2
405,574 - Pf1N1B4_341 0.54 +2.0
405,635 - Pf1N1B4_341 0.61 +0.8
405,635 - Pf1N1B4_341 0.61 -2.0
405,726 - Pf1N1B4_341 0.71 -0.1
405,740 - Pf1N1B4_341 0.73 +0.6
405,815 + +1.8
405,815 + Pf1N1B4_341 0.81 +0.8
405,816 - Pf1N1B4_341 0.81 +1.5
405,966 - +0.8
405,985 + +0.5
405,985 + +1.4

Or see this region's nucleotide sequence