Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3323 and Pf1N1B4_3324 are separated by 30 nucleotides Pf1N1B4_3324 and Pf1N1B4_3325 are separated by 8 nucleotides Pf1N1B4_3325 and Pf1N1B4_3326 are separated by 243 nucleotides
Pf1N1B4_3323 - RNA polymerase sigma-H factor AlgT, at 3,711,515 to 3,712,096
_3323
Pf1N1B4_3324 - Sigma factor RpoE negative regulatory protein RseA, at 3,712,127 to 3,712,714
_3324
Pf1N1B4_3325 - Sigma factor RpoE negative regulatory protein RseB precursor, at 3,712,723 to 3,713,685
_3325
Pf1N1B4_3326 - HtrA protease/chaperone protein, at 3,713,929 to 3,715,359
_3326
Position (kb)
3712
3713
3714 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3712.743 kb on + strand at 3712.931 kb on + strand, within Pf1N1B4_3325 at 3713.412 kb on + strand, within Pf1N1B4_3325 at 3713.412 kb on + strand, within Pf1N1B4_3325 at 3713.574 kb on + strand, within Pf1N1B4_3325 at 3713.649 kb on + strand at 3713.649 kb on + strand at 3713.649 kb on + strand at 3713.649 kb on + strand at 3713.749 kb on + strand at 3713.806 kb on + strand at 3713.808 kb on + strand at 3713.901 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 3,712,743 + +1.5 3,712,931 + Pf1N1B4_3325 0.22 +1.4 3,713,412 + Pf1N1B4_3325 0.72 -0.6 3,713,412 + Pf1N1B4_3325 0.72 -0.9 3,713,574 + Pf1N1B4_3325 0.88 +1.3 3,713,649 + -0.1 3,713,649 + +0.8 3,713,649 + -1.9 3,713,649 + +1.8 3,713,749 + -0.1 3,713,806 + +2.4 3,713,808 + +0.1 3,713,901 + +1.3
Or see this region's nucleotide sequence