Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3308 and Pf1N1B4_3309 are separated by 129 nucleotides Pf1N1B4_3309 and Pf1N1B4_3310 are separated by 115 nucleotides
Pf1N1B4_3308 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,696,875 to 3,697,990
_3308
Pf1N1B4_3309 - Uracil-DNA glycosylase, family 1, at 3,698,120 to 3,698,812
_3309
Pf1N1B4_3310 - Ammonia monooxygenase, at 3,698,928 to 3,699,968
_3310
Position (kb)
3698
3699 Strain fitness (log2 ratio)
-2
-1
0
1 at 3697.641 kb on + strand, within Pf1N1B4_3308 at 3697.642 kb on - strand, within Pf1N1B4_3308 at 3697.642 kb on - strand, within Pf1N1B4_3308 at 3697.642 kb on - strand, within Pf1N1B4_3308 at 3697.674 kb on + strand, within Pf1N1B4_3308 at 3697.674 kb on + strand, within Pf1N1B4_3308 at 3697.779 kb on - strand, within Pf1N1B4_3308 at 3697.779 kb on - strand, within Pf1N1B4_3308 at 3697.779 kb on - strand, within Pf1N1B4_3308 at 3698.004 kb on - strand at 3698.016 kb on + strand at 3698.016 kb on + strand at 3698.017 kb on - strand at 3698.017 kb on - strand at 3698.017 kb on - strand at 3698.019 kb on + strand at 3698.020 kb on - strand at 3698.020 kb on - strand at 3698.032 kb on + strand at 3698.052 kb on - strand at 3698.115 kb on + strand at 3698.116 kb on - strand at 3698.116 kb on - strand at 3698.116 kb on - strand at 3698.116 kb on - strand at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.192 kb on + strand at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.249 kb on + strand, within Pf1N1B4_3309 at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.250 kb on - strand at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.415 kb on - strand, within Pf1N1B4_3309 at 3698.463 kb on - strand, within Pf1N1B4_3309 at 3698.561 kb on + strand, within Pf1N1B4_3309 at 3698.562 kb on - strand, within Pf1N1B4_3309 at 3698.562 kb on - strand, within Pf1N1B4_3309 at 3698.613 kb on + strand, within Pf1N1B4_3309 at 3698.613 kb on + strand, within Pf1N1B4_3309 at 3698.614 kb on - strand, within Pf1N1B4_3309 at 3698.985 kb on + strand at 3698.985 kb on + strand at 3699.478 kb on + strand, within Pf1N1B4_3310 at 3699.479 kb on - strand, within Pf1N1B4_3310 at 3699.681 kb on - strand, within Pf1N1B4_3310
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 3,697,641 + Pf1N1B4_3308 0.69 -1.8 3,697,642 - Pf1N1B4_3308 0.69 -2.2 3,697,642 - Pf1N1B4_3308 0.69 -0.1 3,697,642 - Pf1N1B4_3308 0.69 -1.6 3,697,674 + Pf1N1B4_3308 0.72 -0.4 3,697,674 + Pf1N1B4_3308 0.72 -1.2 3,697,779 - Pf1N1B4_3308 0.81 +0.5 3,697,779 - Pf1N1B4_3308 0.81 -0.3 3,697,779 - Pf1N1B4_3308 0.81 +0.8 3,698,004 - -1.2 3,698,016 + +0.9 3,698,016 + -1.2 3,698,017 - -0.6 3,698,017 - -0.3 3,698,017 - +0.4 3,698,019 + -0.6 3,698,020 - +0.4 3,698,020 - -1.3 3,698,032 + -0.5 3,698,052 - +0.9 3,698,115 + -1.2 3,698,116 - -0.2 3,698,116 - -1.4 3,698,116 - +1.3 3,698,116 - -1.9 3,698,192 + Pf1N1B4_3309 0.10 +0.5 3,698,192 + Pf1N1B4_3309 0.10 -0.3 3,698,192 + Pf1N1B4_3309 0.10 +0.8 3,698,192 + +0.4 3,698,192 + Pf1N1B4_3309 0.10 -0.4 3,698,193 - Pf1N1B4_3309 0.11 +0.1 3,698,193 - Pf1N1B4_3309 0.11 -1.4 3,698,193 - Pf1N1B4_3309 0.11 +0.9 3,698,193 - Pf1N1B4_3309 0.11 -0.1 3,698,193 - Pf1N1B4_3309 0.11 -0.3 3,698,193 - Pf1N1B4_3309 0.11 -0.1 3,698,193 - Pf1N1B4_3309 0.11 -0.1 3,698,193 - Pf1N1B4_3309 0.11 -0.1 3,698,249 + Pf1N1B4_3309 0.19 -0.6 3,698,250 - Pf1N1B4_3309 0.19 +0.9 3,698,250 - Pf1N1B4_3309 0.19 +0.4 3,698,250 - Pf1N1B4_3309 0.19 -0.2 3,698,250 - +0.6 3,698,250 - Pf1N1B4_3309 0.19 +1.4 3,698,415 - Pf1N1B4_3309 0.43 -0.4 3,698,463 - Pf1N1B4_3309 0.49 +0.2 3,698,561 + Pf1N1B4_3309 0.64 -1.1 3,698,562 - Pf1N1B4_3309 0.64 -1.4 3,698,562 - Pf1N1B4_3309 0.64 +0.7 3,698,613 + Pf1N1B4_3309 0.71 -2.5 3,698,613 + Pf1N1B4_3309 0.71 -0.6 3,698,614 - Pf1N1B4_3309 0.71 -1.6 3,698,985 + +0.9 3,698,985 + -1.9 3,699,478 + Pf1N1B4_3310 0.53 -1.1 3,699,479 - Pf1N1B4_3310 0.53 -1.1 3,699,681 - Pf1N1B4_3310 0.72 -0.3
Or see this region's nucleotide sequence