Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3309

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3308 and Pf1N1B4_3309 are separated by 129 nucleotidesPf1N1B4_3309 and Pf1N1B4_3310 are separated by 115 nucleotides Pf1N1B4_3308 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,696,875 to 3,697,990 _3308 Pf1N1B4_3309 - Uracil-DNA glycosylase, family 1, at 3,698,120 to 3,698,812 _3309 Pf1N1B4_3310 - Ammonia monooxygenase, at 3,698,928 to 3,699,968 _3310 Position (kb) 3698 3699Strain fitness (log2 ratio) -2 -1 0 1at 3697.641 kb on + strand, within Pf1N1B4_3308at 3697.642 kb on - strand, within Pf1N1B4_3308at 3697.642 kb on - strand, within Pf1N1B4_3308at 3697.642 kb on - strand, within Pf1N1B4_3308at 3697.674 kb on + strand, within Pf1N1B4_3308at 3697.674 kb on + strand, within Pf1N1B4_3308at 3697.779 kb on - strand, within Pf1N1B4_3308at 3697.779 kb on - strand, within Pf1N1B4_3308at 3697.779 kb on - strand, within Pf1N1B4_3308at 3698.004 kb on - strandat 3698.016 kb on + strandat 3698.016 kb on + strandat 3698.017 kb on - strandat 3698.017 kb on - strandat 3698.017 kb on - strandat 3698.019 kb on + strandat 3698.020 kb on - strandat 3698.020 kb on - strandat 3698.032 kb on + strandat 3698.052 kb on - strandat 3698.115 kb on + strandat 3698.116 kb on - strandat 3698.116 kb on - strandat 3698.116 kb on - strandat 3698.116 kb on - strandat 3698.192 kb on + strand, within Pf1N1B4_3309at 3698.192 kb on + strand, within Pf1N1B4_3309at 3698.192 kb on + strand, within Pf1N1B4_3309at 3698.192 kb on + strandat 3698.192 kb on + strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.193 kb on - strand, within Pf1N1B4_3309at 3698.249 kb on + strand, within Pf1N1B4_3309at 3698.250 kb on - strand, within Pf1N1B4_3309at 3698.250 kb on - strand, within Pf1N1B4_3309at 3698.250 kb on - strand, within Pf1N1B4_3309at 3698.250 kb on - strandat 3698.250 kb on - strand, within Pf1N1B4_3309at 3698.415 kb on - strand, within Pf1N1B4_3309at 3698.463 kb on - strand, within Pf1N1B4_3309at 3698.561 kb on + strand, within Pf1N1B4_3309at 3698.562 kb on - strand, within Pf1N1B4_3309at 3698.562 kb on - strand, within Pf1N1B4_3309at 3698.613 kb on + strand, within Pf1N1B4_3309at 3698.613 kb on + strand, within Pf1N1B4_3309at 3698.614 kb on - strand, within Pf1N1B4_3309at 3698.985 kb on + strandat 3698.985 kb on + strandat 3699.478 kb on + strand, within Pf1N1B4_3310at 3699.479 kb on - strand, within Pf1N1B4_3310at 3699.681 kb on - strand, within Pf1N1B4_3310

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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3,697,641 + Pf1N1B4_3308 0.69 -1.8
3,697,642 - Pf1N1B4_3308 0.69 -2.2
3,697,642 - Pf1N1B4_3308 0.69 -0.1
3,697,642 - Pf1N1B4_3308 0.69 -1.6
3,697,674 + Pf1N1B4_3308 0.72 -0.4
3,697,674 + Pf1N1B4_3308 0.72 -1.2
3,697,779 - Pf1N1B4_3308 0.81 +0.5
3,697,779 - Pf1N1B4_3308 0.81 -0.3
3,697,779 - Pf1N1B4_3308 0.81 +0.8
3,698,004 - -1.2
3,698,016 + +0.9
3,698,016 + -1.2
3,698,017 - -0.6
3,698,017 - -0.3
3,698,017 - +0.4
3,698,019 + -0.6
3,698,020 - +0.4
3,698,020 - -1.3
3,698,032 + -0.5
3,698,052 - +0.9
3,698,115 + -1.2
3,698,116 - -0.2
3,698,116 - -1.4
3,698,116 - +1.3
3,698,116 - -1.9
3,698,192 + Pf1N1B4_3309 0.10 +0.5
3,698,192 + Pf1N1B4_3309 0.10 -0.3
3,698,192 + Pf1N1B4_3309 0.10 +0.8
3,698,192 + +0.4
3,698,192 + Pf1N1B4_3309 0.10 -0.4
3,698,193 - Pf1N1B4_3309 0.11 +0.1
3,698,193 - Pf1N1B4_3309 0.11 -1.4
3,698,193 - Pf1N1B4_3309 0.11 +0.9
3,698,193 - Pf1N1B4_3309 0.11 -0.1
3,698,193 - Pf1N1B4_3309 0.11 -0.3
3,698,193 - Pf1N1B4_3309 0.11 -0.1
3,698,193 - Pf1N1B4_3309 0.11 -0.1
3,698,193 - Pf1N1B4_3309 0.11 -0.1
3,698,249 + Pf1N1B4_3309 0.19 -0.6
3,698,250 - Pf1N1B4_3309 0.19 +0.9
3,698,250 - Pf1N1B4_3309 0.19 +0.4
3,698,250 - Pf1N1B4_3309 0.19 -0.2
3,698,250 - +0.6
3,698,250 - Pf1N1B4_3309 0.19 +1.4
3,698,415 - Pf1N1B4_3309 0.43 -0.4
3,698,463 - Pf1N1B4_3309 0.49 +0.2
3,698,561 + Pf1N1B4_3309 0.64 -1.1
3,698,562 - Pf1N1B4_3309 0.64 -1.4
3,698,562 - Pf1N1B4_3309 0.64 +0.7
3,698,613 + Pf1N1B4_3309 0.71 -2.5
3,698,613 + Pf1N1B4_3309 0.71 -0.6
3,698,614 - Pf1N1B4_3309 0.71 -1.6
3,698,985 + +0.9
3,698,985 + -1.9
3,699,478 + Pf1N1B4_3310 0.53 -1.1
3,699,479 - Pf1N1B4_3310 0.53 -1.1
3,699,681 - Pf1N1B4_3310 0.72 -0.3

Or see this region's nucleotide sequence