Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3196 and Pf1N1B4_3197 are separated by 112 nucleotides Pf1N1B4_3197 and Pf1N1B4_3198 are separated by 24 nucleotides Pf1N1B4_3198 and Pf1N1B4_3199 are separated by 5 nucleotides
Pf1N1B4_3196 - Esterase A, at 3,579,450 to 3,580,595
_3196
Pf1N1B4_3197 - OmpA family protein, at 3,580,708 to 3,581,805
_3197
Pf1N1B4_3198 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 3,581,830 to 3,582,477
_3198
Pf1N1B4_3199 - Ribosomal protein S3, at 3,582,483 to 3,582,719
_3199
Position (kb)
3580
3581
3582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3579.844 kb on - strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.952 kb on + strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.191 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand at 3580.361 kb on - strand, within Pf1N1B4_3196 at 3580.412 kb on - strand, within Pf1N1B4_3196 at 3580.412 kb on - strand, within Pf1N1B4_3196 at 3580.495 kb on - strand at 3580.523 kb on + strand at 3580.523 kb on + strand at 3580.524 kb on - strand at 3580.524 kb on - strand at 3580.537 kb on - strand at 3580.537 kb on - strand at 3580.537 kb on - strand at 3580.537 kb on - strand at 3580.593 kb on + strand at 3580.593 kb on + strand at 3580.594 kb on - strand at 3580.594 kb on - strand at 3580.687 kb on - strand at 3580.719 kb on + strand at 3580.719 kb on + strand at 3580.974 kb on + strand, within Pf1N1B4_3197 at 3580.974 kb on + strand, within Pf1N1B4_3197 at 3581.123 kb on - strand, within Pf1N1B4_3197 at 3581.319 kb on - strand, within Pf1N1B4_3197 at 3581.319 kb on - strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.850 kb on + strand at 3582.475 kb on + strand at 3582.475 kb on + strand at 3582.476 kb on - strand at 3582.476 kb on - strand at 3582.479 kb on + strand at 3582.480 kb on - strand at 3582.480 kb on - strand at 3582.480 kb on - strand at 3582.480 kb on - strand at 3582.480 kb on - strand at 3582.658 kb on + strand, within Pf1N1B4_3199 at 3582.658 kb on + strand, within Pf1N1B4_3199 at 3582.658 kb on + strand at 3582.659 kb on - strand at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand, within Pf1N1B4_3199 at 3582.659 kb on - strand at 3582.676 kb on + strand, within Pf1N1B4_3199 at 3582.676 kb on + strand, within Pf1N1B4_3199 at 3582.676 kb on + strand, within Pf1N1B4_3199 at 3582.677 kb on - strand, within Pf1N1B4_3199 at 3582.677 kb on - strand, within Pf1N1B4_3199 at 3582.677 kb on - strand, within Pf1N1B4_3199 at 3582.677 kb on - strand, within Pf1N1B4_3199 at 3582.677 kb on - strand, within Pf1N1B4_3199 at 3582.712 kb on + strand at 3582.712 kb on + strand at 3582.712 kb on + strand at 3582.713 kb on - strand at 3582.713 kb on - strand at 3582.713 kb on - strand at 3582.713 kb on - strand at 3582.713 kb on - strand at 3582.713 kb on - strand at 3582.713 kb on - strand at 3582.751 kb on + strand at 3582.752 kb on - strand at 3582.752 kb on - strand at 3582.767 kb on + strand at 3582.767 kb on + strand at 3582.767 kb on + strand at 3582.767 kb on + strand at 3582.768 kb on - strand at 3582.792 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 3,579,844 - Pf1N1B4_3196 0.34 +2.0 3,579,930 + Pf1N1B4_3196 0.42 +1.0 3,579,930 + Pf1N1B4_3196 0.42 +0.4 3,579,930 + Pf1N1B4_3196 0.42 +0.8 3,579,930 + Pf1N1B4_3196 0.42 +2.0 3,579,930 + Pf1N1B4_3196 0.42 -2.7 3,579,930 + Pf1N1B4_3196 0.42 -1.0 3,579,931 - Pf1N1B4_3196 0.42 -2.3 3,579,931 - Pf1N1B4_3196 0.42 +1.6 3,579,931 - Pf1N1B4_3196 0.42 -0.6 3,579,931 - Pf1N1B4_3196 0.42 -0.3 3,579,931 - Pf1N1B4_3196 0.42 -0.6 3,579,931 - Pf1N1B4_3196 0.42 -0.6 3,579,952 + Pf1N1B4_3196 0.44 +1.0 3,579,953 - Pf1N1B4_3196 0.44 -0.6 3,579,953 - Pf1N1B4_3196 0.44 -1.0 3,580,181 + Pf1N1B4_3196 0.64 +0.2 3,580,181 + Pf1N1B4_3196 0.64 +0.7 3,580,181 + Pf1N1B4_3196 0.64 +0.0 3,580,182 - Pf1N1B4_3196 0.64 +0.6 3,580,182 - Pf1N1B4_3196 0.64 -0.5 3,580,182 - Pf1N1B4_3196 0.64 +0.4 3,580,182 - Pf1N1B4_3196 0.64 -1.0 3,580,183 + Pf1N1B4_3196 0.64 +0.8 3,580,183 + Pf1N1B4_3196 0.64 +1.0 3,580,190 + Pf1N1B4_3196 0.65 +1.0 3,580,190 + Pf1N1B4_3196 0.65 +1.3 3,580,191 - Pf1N1B4_3196 0.65 +2.0 3,580,273 - Pf1N1B4_3196 0.72 +0.1 3,580,273 - Pf1N1B4_3196 0.72 -2.2 3,580,273 - Pf1N1B4_3196 0.72 +0.0 3,580,273 - +0.0 3,580,361 - Pf1N1B4_3196 0.79 +0.2 3,580,412 - Pf1N1B4_3196 0.84 -1.4 3,580,412 - Pf1N1B4_3196 0.84 -0.5 3,580,495 - -0.3 3,580,523 + -1.1 3,580,523 + +1.0 3,580,524 - -0.4 3,580,524 - -1.3 3,580,537 - -1.8 3,580,537 - +1.6 3,580,537 - -1.3 3,580,537 - -1.8 3,580,593 + +0.3 3,580,593 + -2.0 3,580,594 - -1.6 3,580,594 - -1.7 3,580,687 - -1.0 3,580,719 + -1.3 3,580,719 + -2.3 3,580,974 + Pf1N1B4_3197 0.24 -2.3 3,580,974 + Pf1N1B4_3197 0.24 -1.2 3,581,123 - Pf1N1B4_3197 0.38 -1.3 3,581,319 - Pf1N1B4_3197 0.56 -1.0 3,581,319 - Pf1N1B4_3197 0.56 -3.7 3,581,334 + Pf1N1B4_3197 0.57 -0.2 3,581,334 + Pf1N1B4_3197 0.57 +0.0 3,581,334 + Pf1N1B4_3197 0.57 -1.3 3,581,334 + Pf1N1B4_3197 0.57 -1.2 3,581,335 - Pf1N1B4_3197 0.57 -1.9 3,581,335 - Pf1N1B4_3197 0.57 -1.6 3,581,335 - Pf1N1B4_3197 0.57 -2.2 3,581,335 - Pf1N1B4_3197 0.57 -1.8 3,581,335 - -0.6 3,581,335 - Pf1N1B4_3197 0.57 -0.4 3,581,335 - Pf1N1B4_3197 0.57 -1.7 3,581,560 + Pf1N1B4_3197 0.78 -1.4 3,581,560 + Pf1N1B4_3197 0.78 -0.6 3,581,560 + Pf1N1B4_3197 0.78 -0.3 3,581,560 + Pf1N1B4_3197 0.78 -1.7 3,581,560 + Pf1N1B4_3197 0.78 -1.3 3,581,560 + -1.3 3,581,561 - Pf1N1B4_3197 0.78 -1.3 3,581,561 - Pf1N1B4_3197 0.78 +0.3 3,581,561 - Pf1N1B4_3197 0.78 -1.0 3,581,561 - Pf1N1B4_3197 0.78 -1.0 3,581,850 + -1.6 3,582,475 + -1.6 3,582,475 + -0.6 3,582,476 - -0.6 3,582,476 - -0.3 3,582,479 + -1.0 3,582,480 - -0.7 3,582,480 - +0.5 3,582,480 - +0.2 3,582,480 - -0.4 3,582,480 - +1.3 3,582,658 + Pf1N1B4_3199 0.74 +2.2 3,582,658 + Pf1N1B4_3199 0.74 +1.3 3,582,658 + +2.0 3,582,659 - +3.3 3,582,659 - Pf1N1B4_3199 0.74 +0.3 3,582,659 - Pf1N1B4_3199 0.74 -0.6 3,582,659 - Pf1N1B4_3199 0.74 +0.0 3,582,659 - Pf1N1B4_3199 0.74 -1.8 3,582,659 - Pf1N1B4_3199 0.74 +0.9 3,582,659 - Pf1N1B4_3199 0.74 -0.5 3,582,659 - Pf1N1B4_3199 0.74 +0.4 3,582,659 - +1.0 3,582,676 + Pf1N1B4_3199 0.81 +0.1 3,582,676 + Pf1N1B4_3199 0.81 +1.6 3,582,676 + Pf1N1B4_3199 0.81 -0.7 3,582,677 - Pf1N1B4_3199 0.82 -1.0 3,582,677 - Pf1N1B4_3199 0.82 +0.1 3,582,677 - Pf1N1B4_3199 0.82 +0.4 3,582,677 - Pf1N1B4_3199 0.82 -1.2 3,582,677 - Pf1N1B4_3199 0.82 -0.3 3,582,712 + -0.8 3,582,712 + +0.0 3,582,712 + +1.6 3,582,713 - +0.6 3,582,713 - +1.0 3,582,713 - +0.6 3,582,713 - +0.1 3,582,713 - -1.0 3,582,713 - +0.8 3,582,713 - -0.2 3,582,751 + +1.3 3,582,752 - +0.4 3,582,752 - -0.4 3,582,767 + -1.8 3,582,767 + +0.7 3,582,767 + +1.0 3,582,767 + +0.7 3,582,768 - -0.4 3,582,792 + -2.3
Or see this region's nucleotide sequence