Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_312 and Pf1N1B4_313 are separated by 151 nucleotides Pf1N1B4_313 and Pf1N1B4_314 are separated by 200 nucleotides Pf1N1B4_314 and Pf1N1B4_315 are separated by 10 nucleotides
Pf1N1B4_312 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 365,145 to 367,796
_312
Pf1N1B4_313 - Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 367,948 to 368,493
_313
Pf1N1B4_314 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 368,694 to 370,751
_314
Pf1N1B4_315 - Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 370,762 to 372,456
_315
Position (kb)
368
369
370
371 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 367.725 kb on + strand at 367.725 kb on + strand at 367.725 kb on + strand at 367.813 kb on - strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.896 kb on - strand at 367.896 kb on - strand at 367.896 kb on - strand at 367.949 kb on + strand at 367.968 kb on + strand at 368.046 kb on + strand, within Pf1N1B4_313 at 368.047 kb on - strand, within Pf1N1B4_313 at 368.047 kb on - strand, within Pf1N1B4_313 at 368.177 kb on - strand, within Pf1N1B4_313 at 368.323 kb on + strand, within Pf1N1B4_313 at 368.426 kb on + strand, within Pf1N1B4_313 at 368.479 kb on + strand at 368.479 kb on + strand at 368.480 kb on - strand at 368.506 kb on + strand at 368.506 kb on + strand at 368.506 kb on + strand at 368.507 kb on - strand at 368.698 kb on - strand at 368.774 kb on - strand at 368.774 kb on - strand at 368.948 kb on - strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.984 kb on - strand, within Pf1N1B4_314 at 368.984 kb on - strand, within Pf1N1B4_314 at 369.040 kb on - strand, within Pf1N1B4_314 at 369.673 kb on + strand, within Pf1N1B4_314 at 369.769 kb on + strand, within Pf1N1B4_314 at 369.770 kb on - strand at 370.262 kb on + strand, within Pf1N1B4_314 at 370.651 kb on - strand at 370.734 kb on - strand at 370.763 kb on + strand at 370.763 kb on + strand at 370.764 kb on - strand at 370.764 kb on - strand at 370.959 kb on - strand, within Pf1N1B4_315 at 370.959 kb on - strand, within Pf1N1B4_315 at 370.959 kb on - strand, within Pf1N1B4_315 at 371.066 kb on + strand, within Pf1N1B4_315 at 371.066 kb on + strand, within Pf1N1B4_315 at 371.067 kb on - strand, within Pf1N1B4_315 at 371.067 kb on - strand, within Pf1N1B4_315 at 371.067 kb on - strand, within Pf1N1B4_315 at 371.067 kb on - strand, within Pf1N1B4_315 at 371.067 kb on - strand, within Pf1N1B4_315 at 371.072 kb on + strand, within Pf1N1B4_315 at 371.073 kb on - strand, within Pf1N1B4_315 at 371.073 kb on - strand, within Pf1N1B4_315 at 371.073 kb on - strand, within Pf1N1B4_315 at 371.235 kb on - strand, within Pf1N1B4_315 at 371.273 kb on - strand, within Pf1N1B4_315 at 371.303 kb on + strand, within Pf1N1B4_315 at 371.304 kb on - strand, within Pf1N1B4_315 at 371.538 kb on - strand, within Pf1N1B4_315 at 371.627 kb on + strand, within Pf1N1B4_315 at 371.627 kb on + strand, within Pf1N1B4_315 at 371.627 kb on + strand, within Pf1N1B4_315 at 371.628 kb on - strand, within Pf1N1B4_315 at 371.628 kb on - strand, within Pf1N1B4_315 at 371.628 kb on - strand, within Pf1N1B4_315
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 367,725 + +2.1 367,725 + +1.3 367,725 + +0.8 367,813 - +1.1 367,895 + +1.3 367,895 + +0.3 367,895 + -0.7 367,895 + -0.5 367,895 + -1.5 367,895 + +0.7 367,895 + -1.7 367,895 + +1.8 367,895 + -0.2 367,895 + +0.4 367,895 + -0.2 367,895 + -0.9 367,895 + -2.5 367,895 + +1.2 367,895 + +1.1 367,895 + +0.1 367,896 - +2.2 367,896 - +0.8 367,896 - +2.1 367,949 + +0.8 367,968 + +0.8 368,046 + Pf1N1B4_313 0.18 +0.7 368,047 - Pf1N1B4_313 0.18 -0.7 368,047 - Pf1N1B4_313 0.18 -0.2 368,177 - Pf1N1B4_313 0.42 +0.4 368,323 + Pf1N1B4_313 0.69 -1.2 368,426 + Pf1N1B4_313 0.88 -0.7 368,479 + -0.7 368,479 + +0.1 368,480 - +0.3 368,506 + +0.3 368,506 + -1.2 368,506 + +0.0 368,507 - -2.2 368,698 - -0.4 368,774 - +1.4 368,774 - +0.2 368,948 - Pf1N1B4_314 0.12 +1.9 368,983 + Pf1N1B4_314 0.14 +0.8 368,983 + Pf1N1B4_314 0.14 +0.1 368,983 + Pf1N1B4_314 0.14 +0.5 368,983 + Pf1N1B4_314 0.14 -0.3 368,983 + Pf1N1B4_314 0.14 +1.7 368,984 - Pf1N1B4_314 0.14 +2.5 368,984 - Pf1N1B4_314 0.14 +2.7 369,040 - Pf1N1B4_314 0.17 +1.5 369,673 + Pf1N1B4_314 0.48 +0.0 369,769 + Pf1N1B4_314 0.52 +0.0 369,770 - +0.5 370,262 + Pf1N1B4_314 0.76 -0.4 370,651 - +1.4 370,734 - -0.4 370,763 + -0.7 370,763 + -0.2 370,764 - +0.8 370,764 - +2.9 370,959 - Pf1N1B4_315 0.12 +0.9 370,959 - Pf1N1B4_315 0.12 +2.1 370,959 - Pf1N1B4_315 0.12 +1.1 371,066 + Pf1N1B4_315 0.18 +0.3 371,066 + Pf1N1B4_315 0.18 +0.1 371,067 - Pf1N1B4_315 0.18 +0.8 371,067 - Pf1N1B4_315 0.18 +0.3 371,067 - Pf1N1B4_315 0.18 +1.4 371,067 - Pf1N1B4_315 0.18 +0.6 371,067 - Pf1N1B4_315 0.18 +2.5 371,072 + Pf1N1B4_315 0.18 -1.5 371,073 - Pf1N1B4_315 0.18 +0.6 371,073 - Pf1N1B4_315 0.18 +1.4 371,073 - Pf1N1B4_315 0.18 +0.8 371,235 - Pf1N1B4_315 0.28 +0.3 371,273 - Pf1N1B4_315 0.30 +0.6 371,303 + Pf1N1B4_315 0.32 +1.3 371,304 - Pf1N1B4_315 0.32 +0.0 371,538 - Pf1N1B4_315 0.46 +1.3 371,627 + Pf1N1B4_315 0.51 -0.7 371,627 + Pf1N1B4_315 0.51 +0.5 371,627 + Pf1N1B4_315 0.51 +1.4 371,628 - Pf1N1B4_315 0.51 +1.0 371,628 - Pf1N1B4_315 0.51 +0.8 371,628 - Pf1N1B4_315 0.51 -0.7
Or see this region's nucleotide sequence