Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_30 and Pf1N1B4_31 overlap by 4 nucleotides Pf1N1B4_31 and Pf1N1B4_32 are separated by 179 nucleotides Pf1N1B4_32 and Pf1N1B4_33 are separated by 174 nucleotides
Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899
_30
Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017
_31
Pf1N1B4_32 - Transcriptional regulator, TetR family, at 37,197 to 37,817
_32
Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422
_33
Position (kb) 35
36
37
38 Strain fitness (log2 ratio) -2
-1
0
1
2 at 34.913 kb on - strand, within Pf1N1B4_30 at 34.913 kb on - strand, within Pf1N1B4_30 at 35.033 kb on - strand, within Pf1N1B4_30 at 35.164 kb on + strand, within Pf1N1B4_30 at 35.165 kb on - strand, within Pf1N1B4_30 at 35.320 kb on - strand, within Pf1N1B4_30 at 35.463 kb on + strand, within Pf1N1B4_30 at 35.600 kb on - strand at 35.610 kb on + strand at 35.611 kb on - strand at 35.611 kb on - strand at 35.662 kb on - strand at 35.699 kb on + strand at 35.812 kb on - strand at 36.245 kb on + strand at 36.245 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.560 kb on - strand, within Pf1N1B4_31 at 36.750 kb on + strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 37.017 kb on + strand at 37.267 kb on + strand at 37.465 kb on + strand, within Pf1N1B4_32 at 37.482 kb on - strand at 37.558 kb on - strand, within Pf1N1B4_32 at 37.608 kb on - strand at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.687 kb on - strand, within Pf1N1B4_32 at 37.933 kb on + strand at 37.934 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 34,913 - Pf1N1B4_30 0.68 +0.8 34,913 - Pf1N1B4_30 0.68 -0.5 35,033 - Pf1N1B4_30 0.72 +0.8 35,164 + Pf1N1B4_30 0.76 +1.3 35,165 - Pf1N1B4_30 0.76 +0.4 35,320 - Pf1N1B4_30 0.81 +0.0 35,463 + Pf1N1B4_30 0.86 -1.5 35,600 - -0.2 35,610 + +1.6 35,611 - +0.2 35,611 - -0.2 35,662 - +0.8 35,699 + +1.3 35,812 - -1.8 36,245 + +2.4 36,245 + Pf1N1B4_31 0.31 -1.2 36,370 + Pf1N1B4_31 0.42 +1.0 36,370 + Pf1N1B4_31 0.42 -0.5 36,479 - Pf1N1B4_31 0.52 +0.2 36,479 - Pf1N1B4_31 0.52 +0.5 36,479 - Pf1N1B4_31 0.52 -0.0 36,479 - Pf1N1B4_31 0.52 +1.7 36,560 - Pf1N1B4_31 0.59 -0.5 36,750 + Pf1N1B4_31 0.76 +0.8 36,751 - Pf1N1B4_31 0.76 -1.4 36,751 - Pf1N1B4_31 0.76 +1.3 37,017 + +0.2 37,267 + -0.8 37,465 + Pf1N1B4_32 0.43 +0.2 37,482 - -1.0 37,558 - Pf1N1B4_32 0.58 +1.3 37,608 - -1.2 37,657 + Pf1N1B4_32 0.74 +2.1 37,657 + Pf1N1B4_32 0.74 -0.8 37,657 + Pf1N1B4_32 0.74 -0.8 37,658 - Pf1N1B4_32 0.74 -0.4 37,658 - -0.8 37,658 - +2.4 37,658 - -1.8 37,658 - Pf1N1B4_32 0.74 -1.6 37,658 - Pf1N1B4_32 0.74 -0.5 37,658 - Pf1N1B4_32 0.74 -1.8 37,658 - -0.8 37,687 - Pf1N1B4_32 0.79 -0.4 37,933 + +0.2 37,934 - +2.1
Or see this region's nucleotide sequence