Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3005 and Pf1N1B4_3006 overlap by 4 nucleotides Pf1N1B4_3006 and Pf1N1B4_3007 are separated by 43 nucleotides Pf1N1B4_3007 and Pf1N1B4_3008 are separated by 63 nucleotides
Pf1N1B4_3005 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 3,379,102 to 3,380,166
_3005
Pf1N1B4_3006 - tRNA-guanine transglycosylase (EC 2.4.2.29), at 3,380,163 to 3,381,296
_3006
Pf1N1B4_3007 - Preprotein translocase subunit YajC (TC 3.A.5.1.1), at 3,381,340 to 3,381,675
_3007
Pf1N1B4_3008 - Protein-export membrane protein SecD (TC 3.A.5.1.1), at 3,381,739 to 3,383,607
_3008
Position (kb)
3380
3381
3382 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.548 kb on - strand, within Pf1N1B4_3005 at 3379.548 kb on - strand, within Pf1N1B4_3005 at 3379.548 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.858 kb on - strand, within Pf1N1B4_3005 at 3379.924 kb on - strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3380.050 kb on + strand, within Pf1N1B4_3005 at 3380.050 kb on + strand, within Pf1N1B4_3005 at 3380.050 kb on + strand, within Pf1N1B4_3005 at 3380.050 kb on + strand, within Pf1N1B4_3005 at 3380.050 kb on + strand, within Pf1N1B4_3005 at 3380.051 kb on - strand, within Pf1N1B4_3005 at 3380.052 kb on + strand, within Pf1N1B4_3005 at 3380.053 kb on - strand, within Pf1N1B4_3005 at 3380.089 kb on + strand at 3380.106 kb on + strand at 3380.106 kb on + strand at 3380.106 kb on + strand at 3380.107 kb on - strand at 3380.107 kb on - strand at 3380.115 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.127 kb on + strand at 3380.128 kb on - strand at 3380.128 kb on - strand at 3380.283 kb on + strand, within Pf1N1B4_3006 at 3380.284 kb on - strand at 3380.742 kb on - strand, within Pf1N1B4_3006 at 3380.862 kb on + strand, within Pf1N1B4_3006 at 3380.862 kb on + strand, within Pf1N1B4_3006 at 3380.903 kb on + strand at 3381.014 kb on + strand, within Pf1N1B4_3006 at 3381.014 kb on + strand, within Pf1N1B4_3006 at 3381.014 kb on + strand, within Pf1N1B4_3006 at 3381.014 kb on + strand, within Pf1N1B4_3006 at 3381.087 kb on + strand, within Pf1N1B4_3006 at 3381.087 kb on + strand, within Pf1N1B4_3006 at 3381.087 kb on + strand, within Pf1N1B4_3006 at 3381.087 kb on + strand at 3381.087 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.089 kb on + strand, within Pf1N1B4_3006 at 3381.114 kb on + strand, within Pf1N1B4_3006 at 3381.114 kb on + strand, within Pf1N1B4_3006 at 3381.114 kb on + strand, within Pf1N1B4_3006 at 3381.114 kb on + strand, within Pf1N1B4_3006 at 3381.114 kb on + strand, within Pf1N1B4_3006 at 3381.114 kb on + strand, within Pf1N1B4_3006 at 3381.164 kb on + strand, within Pf1N1B4_3006 at 3381.164 kb on + strand, within Pf1N1B4_3006 at 3381.164 kb on + strand, within Pf1N1B4_3006 at 3381.164 kb on + strand, within Pf1N1B4_3006 at 3381.197 kb on + strand at 3381.197 kb on + strand at 3381.197 kb on + strand at 3381.197 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.230 kb on + strand at 3381.258 kb on + strand at 3381.274 kb on + strand at 3381.673 kb on + strand at 3381.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 3,379,543 + Pf1N1B4_3005 0.41 +2.3 3,379,543 + Pf1N1B4_3005 0.41 +2.2 3,379,543 + Pf1N1B4_3005 0.41 +1.0 3,379,543 + Pf1N1B4_3005 0.41 +2.0 3,379,543 + Pf1N1B4_3005 0.41 +2.4 3,379,543 + Pf1N1B4_3005 0.41 +2.7 3,379,543 + Pf1N1B4_3005 0.41 +1.9 3,379,543 + -0.5 3,379,543 + Pf1N1B4_3005 0.41 +2.6 3,379,543 + Pf1N1B4_3005 0.41 +1.3 3,379,543 + Pf1N1B4_3005 0.41 +0.8 3,379,544 - Pf1N1B4_3005 0.42 +2.7 3,379,544 - Pf1N1B4_3005 0.42 +3.4 3,379,544 - Pf1N1B4_3005 0.42 -0.5 3,379,544 - Pf1N1B4_3005 0.42 +3.6 3,379,544 - Pf1N1B4_3005 0.42 +3.8 3,379,544 - Pf1N1B4_3005 0.42 +2.0 3,379,544 - Pf1N1B4_3005 0.42 +2.5 3,379,544 - Pf1N1B4_3005 0.42 +2.0 3,379,544 - Pf1N1B4_3005 0.42 +3.6 3,379,544 - Pf1N1B4_3005 0.42 +3.0 3,379,544 - Pf1N1B4_3005 0.42 +2.9 3,379,545 + Pf1N1B4_3005 0.42 +2.4 3,379,545 + +3.2 3,379,545 + Pf1N1B4_3005 0.42 +2.8 3,379,545 + Pf1N1B4_3005 0.42 +4.2 3,379,545 + Pf1N1B4_3005 0.42 +0.8 3,379,545 + Pf1N1B4_3005 0.42 +1.5 3,379,545 + Pf1N1B4_3005 0.42 +2.0 3,379,545 + Pf1N1B4_3005 0.42 +2.7 3,379,545 + Pf1N1B4_3005 0.42 +1.2 3,379,545 + Pf1N1B4_3005 0.42 +1.9 3,379,545 + +5.0 3,379,545 + Pf1N1B4_3005 0.42 +2.6 3,379,545 + Pf1N1B4_3005 0.42 +0.8 3,379,545 + Pf1N1B4_3005 0.42 +2.1 3,379,545 + +5.0 3,379,545 + Pf1N1B4_3005 0.42 +1.0 3,379,545 + Pf1N1B4_3005 0.42 +2.8 3,379,545 + Pf1N1B4_3005 0.42 +1.9 3,379,546 - Pf1N1B4_3005 0.42 +1.0 3,379,546 - Pf1N1B4_3005 0.42 +3.1 3,379,546 - Pf1N1B4_3005 0.42 +2.0 3,379,546 - Pf1N1B4_3005 0.42 +2.4 3,379,546 - Pf1N1B4_3005 0.42 +2.3 3,379,546 - Pf1N1B4_3005 0.42 -1.0 3,379,546 - Pf1N1B4_3005 0.42 +2.0 3,379,547 + Pf1N1B4_3005 0.42 +2.0 3,379,547 + Pf1N1B4_3005 0.42 +2.3 3,379,547 + Pf1N1B4_3005 0.42 +1.2 3,379,547 + Pf1N1B4_3005 0.42 +3.6 3,379,547 + Pf1N1B4_3005 0.42 +2.3 3,379,547 + Pf1N1B4_3005 0.42 +2.6 3,379,547 + Pf1N1B4_3005 0.42 +1.9 3,379,547 + Pf1N1B4_3005 0.42 +3.3 3,379,547 + Pf1N1B4_3005 0.42 +2.9 3,379,547 + Pf1N1B4_3005 0.42 +2.6 3,379,548 - Pf1N1B4_3005 0.42 +4.0 3,379,548 - Pf1N1B4_3005 0.42 +2.0 3,379,548 - Pf1N1B4_3005 0.42 +2.8 3,379,595 - Pf1N1B4_3005 0.46 +4.7 3,379,595 - Pf1N1B4_3005 0.46 +4.2 3,379,595 - Pf1N1B4_3005 0.46 +3.9 3,379,595 - Pf1N1B4_3005 0.46 +2.1 3,379,858 - Pf1N1B4_3005 0.71 +2.0 3,379,924 - Pf1N1B4_3005 0.77 +0.4 3,379,929 + Pf1N1B4_3005 0.78 +1.3 3,379,929 + Pf1N1B4_3005 0.78 +1.6 3,379,929 + Pf1N1B4_3005 0.78 +3.5 3,379,929 + Pf1N1B4_3005 0.78 +1.8 3,379,929 + Pf1N1B4_3005 0.78 +2.3 3,379,929 + Pf1N1B4_3005 0.78 +3.1 3,379,929 + Pf1N1B4_3005 0.78 +1.3 3,379,930 - Pf1N1B4_3005 0.78 +3.5 3,379,930 - Pf1N1B4_3005 0.78 +2.4 3,379,930 - Pf1N1B4_3005 0.78 +3.0 3,379,930 - Pf1N1B4_3005 0.78 +3.1 3,379,930 - Pf1N1B4_3005 0.78 +2.6 3,380,050 + Pf1N1B4_3005 0.89 +2.6 3,380,050 + Pf1N1B4_3005 0.89 +1.9 3,380,050 + Pf1N1B4_3005 0.89 +3.2 3,380,050 + Pf1N1B4_3005 0.89 +2.3 3,380,050 + Pf1N1B4_3005 0.89 -1.5 3,380,051 - Pf1N1B4_3005 0.89 +2.4 3,380,052 + Pf1N1B4_3005 0.89 +2.0 3,380,053 - Pf1N1B4_3005 0.89 +2.0 3,380,089 + +2.7 3,380,106 + +1.7 3,380,106 + +3.5 3,380,106 + +2.4 3,380,107 - +2.4 3,380,107 - +2.0 3,380,115 + -0.4 3,380,127 + +1.0 3,380,127 + -0.1 3,380,127 + -1.0 3,380,127 + +0.1 3,380,127 + +0.3 3,380,127 + +0.4 3,380,127 + +1.0 3,380,127 + -0.8 3,380,128 - +3.0 3,380,128 - +0.9 3,380,283 + Pf1N1B4_3006 0.11 +1.3 3,380,284 - +0.7 3,380,742 - Pf1N1B4_3006 0.51 +1.5 3,380,862 + Pf1N1B4_3006 0.62 +1.7 3,380,862 + Pf1N1B4_3006 0.62 +3.0 3,380,903 + +1.6 3,381,014 + Pf1N1B4_3006 0.75 +3.3 3,381,014 + Pf1N1B4_3006 0.75 +3.3 3,381,014 + Pf1N1B4_3006 0.75 +2.5 3,381,014 + Pf1N1B4_3006 0.75 +2.3 3,381,087 + Pf1N1B4_3006 0.81 +2.1 3,381,087 + Pf1N1B4_3006 0.81 +1.7 3,381,087 + Pf1N1B4_3006 0.81 +1.5 3,381,087 + +2.0 3,381,087 + Pf1N1B4_3006 0.81 +1.7 3,381,089 + Pf1N1B4_3006 0.82 +2.2 3,381,089 + Pf1N1B4_3006 0.82 +2.8 3,381,089 + Pf1N1B4_3006 0.82 +2.4 3,381,089 + Pf1N1B4_3006 0.82 +2.2 3,381,089 + Pf1N1B4_3006 0.82 +0.8 3,381,089 + Pf1N1B4_3006 0.82 +2.2 3,381,089 + Pf1N1B4_3006 0.82 +3.1 3,381,089 + Pf1N1B4_3006 0.82 +1.3 3,381,114 + Pf1N1B4_3006 0.84 +1.9 3,381,114 + Pf1N1B4_3006 0.84 +3.4 3,381,114 + Pf1N1B4_3006 0.84 +3.9 3,381,114 + Pf1N1B4_3006 0.84 +2.0 3,381,114 + Pf1N1B4_3006 0.84 +3.5 3,381,114 + Pf1N1B4_3006 0.84 +3.2 3,381,164 + Pf1N1B4_3006 0.88 +3.6 3,381,164 + Pf1N1B4_3006 0.88 +2.7 3,381,164 + Pf1N1B4_3006 0.88 +2.5 3,381,164 + Pf1N1B4_3006 0.88 +3.4 3,381,197 + +3.0 3,381,197 + +0.2 3,381,197 + +2.5 3,381,197 + +3.0 3,381,230 + +3.2 3,381,230 + +1.0 3,381,230 + +3.8 3,381,230 + +4.0 3,381,230 + +1.5 3,381,230 + +3.9 3,381,230 + +3.0 3,381,230 + +2.8 3,381,230 + +0.0 3,381,230 + +1.7 3,381,258 + +2.3 3,381,274 + +0.8 3,381,673 + -0.2 3,381,673 + -1.1
Or see this region's nucleotide sequence