Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_27 and Pf1N1B4_28 are separated by 235 nucleotides Pf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotides Pf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotides Pf1N1B4_5164 and Pf1N1B4_30 are separated by 102 nucleotides
Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749
_27
Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185
_28
Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412
_29
Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710
_5164
Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899
_30
Position (kb)
32
33 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.406 kb on - strand, within Pf1N1B4_27 at 31.606 kb on + strand, within Pf1N1B4_27 at 31.631 kb on + strand at 31.631 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.762 kb on + strand at 31.781 kb on - strand at 31.797 kb on + strand at 31.798 kb on - strand at 31.810 kb on + strand at 31.892 kb on - strand at 31.952 kb on + strand at 31.952 kb on + strand at 31.958 kb on + strand at 31.958 kb on + strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 32.001 kb on + strand at 32.002 kb on - strand at 32.144 kb on + strand, within Pf1N1B4_28 at 32.170 kb on + strand at 32.171 kb on - strand at 32.171 kb on - strand at 32.223 kb on - strand, within Pf1N1B4_29 at 32.223 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.463 kb on + strand at 32.517 kb on + strand at 32.526 kb on - strand at 32.811 kb on + strand at 32.844 kb on + strand at 32.845 kb on - strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.943 kb on + strand at 32.944 kb on - strand at 33.271 kb on + strand at 33.272 kb on - strand at 33.275 kb on - strand at 33.275 kb on - strand, within Pf1N1B4_30
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 31,405 + Pf1N1B4_27 0.71 -0.8 31,405 + Pf1N1B4_27 0.71 -1.2 31,405 + Pf1N1B4_27 0.71 -2.1 31,405 + Pf1N1B4_27 0.71 +0.8 31,406 - Pf1N1B4_27 0.71 -0.5 31,606 + Pf1N1B4_27 0.88 -0.4 31,631 + +0.4 31,631 + -1.2 31,660 + -0.2 31,660 + +1.2 31,660 + -0.2 31,660 + -0.8 31,660 + -1.8 31,661 - -1.8 31,661 - +0.8 31,661 - +0.0 31,661 - -0.2 31,762 + -0.2 31,781 - +1.4 31,797 + -0.3 31,798 - +0.0 31,810 + +0.8 31,892 - -1.0 31,952 + +0.5 31,952 + +0.8 31,958 + +1.8 31,958 + -0.9 31,959 - +1.4 31,959 - +0.5 31,959 - +0.3 31,959 - -1.1 31,959 - -0.8 32,001 + +0.8 32,002 - +1.4 32,144 + Pf1N1B4_28 0.79 -1.8 32,170 + -0.2 32,171 - +0.6 32,171 - +0.6 32,223 - Pf1N1B4_29 0.18 +0.4 32,223 - Pf1N1B4_29 0.18 -1.7 32,232 - Pf1N1B4_29 0.22 -0.2 32,232 - Pf1N1B4_29 0.22 -1.8 32,232 - Pf1N1B4_29 0.22 -1.5 32,463 + -0.2 32,517 + -2.3 32,526 - -0.8 32,811 + -2.8 32,844 + -0.6 32,845 - +0.2 32,880 + -0.3 32,880 + -1.2 32,880 + +2.1 32,881 - -0.2 32,881 - -0.8 32,881 - -1.6 32,943 + -1.2 32,944 - -0.4 33,271 + -0.2 33,272 - -0.2 33,275 - +2.8 33,275 - Pf1N1B4_30 0.15 -1.5
Or see this region's nucleotide sequence