Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_276

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_274 and Pf1N1B4_275 are separated by 69 nucleotidesPf1N1B4_275 and Pf1N1B4_276 are separated by 350 nucleotidesPf1N1B4_276 and Pf1N1B4_277 are separated by 32 nucleotides Pf1N1B4_274 - Transcriptional regulator, LysR family, at 333,631 to 334,623 _274 Pf1N1B4_275 - Organic hydroperoxide resistance transcriptional regulator, at 334,693 to 335,154 _275 Pf1N1B4_276 - Organic hydroperoxide resistance protein, at 335,505 to 335,924 _276 Pf1N1B4_277 - Lipase, at 335,957 to 336,973 _277 Position (kb) 335 336Strain fitness (log2 ratio) -2 -1 0 1 2at 334.552 kb on + strandat 334.622 kb on - strandat 334.795 kb on + strand, within Pf1N1B4_275at 334.796 kb on - strand, within Pf1N1B4_275at 334.796 kb on - strand, within Pf1N1B4_275at 334.828 kb on + strand, within Pf1N1B4_275at 334.994 kb on + strand, within Pf1N1B4_275at 334.994 kb on + strand, within Pf1N1B4_275at 334.995 kb on - strandat 335.130 kb on + strandat 335.131 kb on - strandat 335.131 kb on - strandat 335.145 kb on + strandat 335.348 kb on + strandat 335.348 kb on + strandat 335.348 kb on + strandat 335.349 kb on - strandat 335.349 kb on - strandat 335.356 kb on + strandat 335.357 kb on - strandat 335.421 kb on - strandat 335.518 kb on - strandat 335.845 kb on + strand, within Pf1N1B4_276at 335.845 kb on + strand, within Pf1N1B4_276at 335.846 kb on - strand, within Pf1N1B4_276at 335.846 kb on - strand, within Pf1N1B4_276at 335.846 kb on - strand, within Pf1N1B4_276at 335.922 kb on + strandat 335.922 kb on + strandat 335.923 kb on - strandat 335.923 kb on - strandat 335.923 kb on - strandat 335.923 kb on - strandat 335.956 kb on + strandat 335.956 kb on + strandat 335.956 kb on + strandat 335.957 kb on - strandat 335.957 kb on - strandat 335.957 kb on - strandat 335.957 kb on - strandat 336.063 kb on + strand, within Pf1N1B4_277at 336.063 kb on + strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strandat 336.065 kb on - strand, within Pf1N1B4_277at 336.147 kb on + strand, within Pf1N1B4_277at 336.368 kb on + strandat 336.368 kb on + strand, within Pf1N1B4_277at 336.368 kb on + strand, within Pf1N1B4_277at 336.368 kb on + strand, within Pf1N1B4_277at 336.368 kb on + strand, within Pf1N1B4_277at 336.369 kb on - strand, within Pf1N1B4_277at 336.369 kb on - strand, within Pf1N1B4_277at 336.393 kb on - strandat 336.416 kb on + strand, within Pf1N1B4_277at 336.417 kb on - strand, within Pf1N1B4_277at 336.563 kb on + strand, within Pf1N1B4_277at 336.563 kb on + strandat 336.614 kb on + strand, within Pf1N1B4_277at 336.614 kb on + strand, within Pf1N1B4_277at 336.614 kb on + strandat 336.614 kb on + strand, within Pf1N1B4_277at 336.614 kb on + strand, within Pf1N1B4_277at 336.615 kb on - strand, within Pf1N1B4_277at 336.615 kb on - strand, within Pf1N1B4_277at 336.615 kb on - strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strandat 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.677 kb on + strand, within Pf1N1B4_277at 336.678 kb on - strand, within Pf1N1B4_277at 336.678 kb on - strand, within Pf1N1B4_277at 336.678 kb on - strand, within Pf1N1B4_277at 336.678 kb on - strand, within Pf1N1B4_277at 336.678 kb on - strand, within Pf1N1B4_277at 336.710 kb on + strand, within Pf1N1B4_277at 336.710 kb on + strand, within Pf1N1B4_277at 336.710 kb on + strand, within Pf1N1B4_277at 336.711 kb on - strand, within Pf1N1B4_277at 336.711 kb on - strand, within Pf1N1B4_277at 336.711 kb on - strand, within Pf1N1B4_277at 336.711 kb on - strand, within Pf1N1B4_277at 336.789 kb on + strand, within Pf1N1B4_277at 336.789 kb on + strand, within Pf1N1B4_277at 336.789 kb on + strand, within Pf1N1B4_277at 336.789 kb on + strand, within Pf1N1B4_277at 336.789 kb on + strand, within Pf1N1B4_277at 336.789 kb on + strand, within Pf1N1B4_277at 336.790 kb on - strand, within Pf1N1B4_277at 336.790 kb on - strand, within Pf1N1B4_277at 336.813 kb on - strand, within Pf1N1B4_277at 336.860 kb on + strand, within Pf1N1B4_277at 336.861 kb on - strandat 336.861 kb on - strand, within Pf1N1B4_277at 336.882 kb on - strandat 336.882 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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334,552 + +1.8
334,622 - -1.8
334,795 + Pf1N1B4_275 0.22 +0.8
334,796 - Pf1N1B4_275 0.22 -1.3
334,796 - Pf1N1B4_275 0.22 +1.8
334,828 + Pf1N1B4_275 0.29 -0.2
334,994 + Pf1N1B4_275 0.65 +0.8
334,994 + Pf1N1B4_275 0.65 -0.2
334,995 - -0.2
335,130 + -1.2
335,131 - +1.4
335,131 - +0.5
335,145 + -0.8
335,348 + +0.1
335,348 + +1.4
335,348 + -0.5
335,349 - +1.8
335,349 - -1.5
335,356 + -0.2
335,357 - +0.8
335,421 - -0.9
335,518 - -1.0
335,845 + Pf1N1B4_276 0.81 -2.0
335,845 + Pf1N1B4_276 0.81 +0.8
335,846 - Pf1N1B4_276 0.81 -2.0
335,846 - Pf1N1B4_276 0.81 -1.5
335,846 - Pf1N1B4_276 0.81 +1.2
335,922 + -2.2
335,922 + -1.2
335,923 - -0.8
335,923 - +0.2
335,923 - +0.5
335,923 - -1.8
335,956 + -0.8
335,956 + -1.6
335,956 + -1.8
335,957 - -0.2
335,957 - -1.0
335,957 - +0.2
335,957 - -0.9
336,063 + Pf1N1B4_277 0.10 -0.8
336,063 + Pf1N1B4_277 0.10 -0.3
336,064 - Pf1N1B4_277 0.11 -0.2
336,064 - Pf1N1B4_277 0.11 -0.2
336,064 - Pf1N1B4_277 0.11 -0.2
336,064 - Pf1N1B4_277 0.11 -0.0
336,064 - Pf1N1B4_277 0.11 +1.2
336,064 - -0.8
336,065 - Pf1N1B4_277 0.11 +0.1
336,147 + Pf1N1B4_277 0.19 -0.2
336,368 + +0.8
336,368 + Pf1N1B4_277 0.40 -0.2
336,368 + Pf1N1B4_277 0.40 -1.2
336,368 + Pf1N1B4_277 0.40 -0.4
336,368 + Pf1N1B4_277 0.40 -1.8
336,369 - Pf1N1B4_277 0.41 +0.6
336,369 - Pf1N1B4_277 0.41 -1.2
336,393 - -1.5
336,416 + Pf1N1B4_277 0.45 -0.1
336,417 - Pf1N1B4_277 0.45 +1.4
336,563 + Pf1N1B4_277 0.60 +0.4
336,563 + -0.8
336,614 + Pf1N1B4_277 0.65 -0.6
336,614 + Pf1N1B4_277 0.65 -0.8
336,614 + +1.8
336,614 + Pf1N1B4_277 0.65 +0.2
336,614 + Pf1N1B4_277 0.65 -0.2
336,615 - Pf1N1B4_277 0.65 -0.4
336,615 - Pf1N1B4_277 0.65 -0.5
336,615 - Pf1N1B4_277 0.65 -1.0
336,677 + Pf1N1B4_277 0.71 -0.2
336,677 + Pf1N1B4_277 0.71 -1.2
336,677 + Pf1N1B4_277 0.71 -0.5
336,677 + Pf1N1B4_277 0.71 +0.5
336,677 + Pf1N1B4_277 0.71 +0.2
336,677 + Pf1N1B4_277 0.71 +1.3
336,677 + -0.5
336,677 + Pf1N1B4_277 0.71 -0.5
336,677 + Pf1N1B4_277 0.71 -2.3
336,677 + Pf1N1B4_277 0.71 -0.8
336,677 + Pf1N1B4_277 0.71 +0.8
336,678 - Pf1N1B4_277 0.71 +0.1
336,678 - Pf1N1B4_277 0.71 +0.1
336,678 - Pf1N1B4_277 0.71 +1.2
336,678 - Pf1N1B4_277 0.71 +2.0
336,678 - Pf1N1B4_277 0.71 +1.0
336,710 + Pf1N1B4_277 0.74 +1.1
336,710 + Pf1N1B4_277 0.74 -0.8
336,710 + Pf1N1B4_277 0.74 -0.6
336,711 - Pf1N1B4_277 0.74 +0.8
336,711 - Pf1N1B4_277 0.74 +0.5
336,711 - Pf1N1B4_277 0.74 +0.3
336,711 - Pf1N1B4_277 0.74 -1.2
336,789 + Pf1N1B4_277 0.82 +0.0
336,789 + Pf1N1B4_277 0.82 +0.4
336,789 + Pf1N1B4_277 0.82 -2.0
336,789 + Pf1N1B4_277 0.82 +2.4
336,789 + Pf1N1B4_277 0.82 +0.8
336,789 + Pf1N1B4_277 0.82 -0.3
336,790 - Pf1N1B4_277 0.82 -0.7
336,790 - Pf1N1B4_277 0.82 +0.6
336,813 - Pf1N1B4_277 0.84 +1.8
336,860 + Pf1N1B4_277 0.89 +1.6
336,861 - -0.8
336,861 - Pf1N1B4_277 0.89 -0.2
336,882 - +1.0
336,882 - -0.0

Or see this region's nucleotide sequence