Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_273

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_271 and Pf1N1B4_272 are separated by 96 nucleotidesPf1N1B4_272 and Pf1N1B4_273 overlap by 11 nucleotidesPf1N1B4_273 and Pf1N1B4_274 are separated by 72 nucleotides Pf1N1B4_271 - FIG00953957: hypothetical protein, at 331,653 to 331,868 _271 Pf1N1B4_272 - Transcriptional regulator, LysR family, at 331,965 to 332,819 _272 Pf1N1B4_273 - putative membrane protein, at 332,809 to 333,558 _273 Pf1N1B4_274 - Transcriptional regulator, LysR family, at 333,631 to 334,623 _274 Position (kb) 332 333 334Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 331.846 kb on - strand, within Pf1N1B4_271at 331.871 kb on + strandat 331.872 kb on - strandat 331.945 kb on + strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.946 kb on - strandat 331.996 kb on + strandat 331.997 kb on - strandat 331.997 kb on - strandat 331.997 kb on - strandat 331.997 kb on - strandat 331.997 kb on - strandat 331.997 kb on - strandat 331.997 kb on - strandat 332.036 kb on + strandat 332.037 kb on - strandat 332.173 kb on + strand, within Pf1N1B4_272at 332.174 kb on - strand, within Pf1N1B4_272at 332.174 kb on - strand, within Pf1N1B4_272at 332.174 kb on - strand, within Pf1N1B4_272at 332.174 kb on - strand, within Pf1N1B4_272at 332.185 kb on - strand, within Pf1N1B4_272at 332.226 kb on - strand, within Pf1N1B4_272at 332.226 kb on - strand, within Pf1N1B4_272at 332.281 kb on - strandat 332.595 kb on + strandat 332.595 kb on + strand, within Pf1N1B4_272at 332.595 kb on + strand, within Pf1N1B4_272at 332.596 kb on - strand, within Pf1N1B4_272at 332.596 kb on - strand, within Pf1N1B4_272at 332.596 kb on - strand, within Pf1N1B4_272at 332.818 kb on - strandat 332.981 kb on + strand, within Pf1N1B4_273at 332.981 kb on + strandat 332.982 kb on - strand, within Pf1N1B4_273at 332.982 kb on - strand, within Pf1N1B4_273at 332.982 kb on - strandat 332.982 kb on - strand, within Pf1N1B4_273at 332.982 kb on - strand, within Pf1N1B4_273at 332.982 kb on - strand, within Pf1N1B4_273at 333.003 kb on + strand, within Pf1N1B4_273at 333.004 kb on - strand, within Pf1N1B4_273at 333.165 kb on - strand, within Pf1N1B4_273at 333.219 kb on - strand, within Pf1N1B4_273at 333.539 kb on + strandat 333.539 kb on + strandat 333.539 kb on + strandat 333.539 kb on + strandat 333.540 kb on - strandat 333.540 kb on - strandat 333.540 kb on - strandat 333.590 kb on + strandat 333.591 kb on - strandat 333.933 kb on + strandat 333.934 kb on - strand, within Pf1N1B4_274at 334.109 kb on + strand, within Pf1N1B4_274at 334.109 kb on + strand, within Pf1N1B4_274at 334.109 kb on + strand, within Pf1N1B4_274at 334.110 kb on - strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.113 kb on - strand, within Pf1N1B4_274at 334.113 kb on - strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.431 kb on - strand, within Pf1N1B4_274at 334.431 kb on - strandat 334.431 kb on - strandat 334.431 kb on - strand, within Pf1N1B4_274at 334.433 kb on + strand, within Pf1N1B4_274at 334.498 kb on + strandat 334.498 kb on + strand, within Pf1N1B4_274at 334.498 kb on + strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.552 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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331,846 - Pf1N1B4_271 0.89 +0.3
331,871 + -2.0
331,872 - -0.2
331,945 + -3.4
331,946 - +0.6
331,946 - -0.4
331,946 - -0.5
331,946 - -1.1
331,946 - +0.1
331,946 - +1.4
331,946 - +1.0
331,946 - +0.8
331,946 - +0.2
331,996 + -3.4
331,997 - -0.6
331,997 - -0.8
331,997 - -0.5
331,997 - -0.0
331,997 - +0.8
331,997 - -1.8
331,997 - -0.8
332,036 + -2.5
332,037 - -2.0
332,173 + Pf1N1B4_272 0.24 +0.5
332,174 - Pf1N1B4_272 0.24 -0.5
332,174 - Pf1N1B4_272 0.24 +0.9
332,174 - Pf1N1B4_272 0.24 +0.2
332,174 - Pf1N1B4_272 0.24 +0.2
332,185 - Pf1N1B4_272 0.26 -0.3
332,226 - Pf1N1B4_272 0.31 -1.8
332,226 - Pf1N1B4_272 0.31 +1.6
332,281 - +0.8
332,595 + +0.8
332,595 + Pf1N1B4_272 0.74 -0.5
332,595 + Pf1N1B4_272 0.74 -0.5
332,596 - Pf1N1B4_272 0.74 +0.1
332,596 - Pf1N1B4_272 0.74 +0.2
332,596 - Pf1N1B4_272 0.74 +0.8
332,818 - +1.3
332,981 + Pf1N1B4_273 0.23 +0.7
332,981 + -0.2
332,982 - Pf1N1B4_273 0.23 -1.9
332,982 - Pf1N1B4_273 0.23 -0.6
332,982 - +4.0
332,982 - Pf1N1B4_273 0.23 +0.0
332,982 - Pf1N1B4_273 0.23 -0.2
332,982 - Pf1N1B4_273 0.23 +0.6
333,003 + Pf1N1B4_273 0.26 +2.6
333,004 - Pf1N1B4_273 0.26 -1.2
333,165 - Pf1N1B4_273 0.47 +0.8
333,219 - Pf1N1B4_273 0.55 +2.9
333,539 + -0.2
333,539 + -1.8
333,539 + +0.4
333,539 + -1.0
333,540 - -0.8
333,540 - +1.0
333,540 - +0.8
333,590 + -0.2
333,591 - -2.2
333,933 + +0.1
333,934 - Pf1N1B4_274 0.31 +0.2
334,109 + Pf1N1B4_274 0.48 -1.5
334,109 + Pf1N1B4_274 0.48 -0.8
334,109 + Pf1N1B4_274 0.48 -0.2
334,110 - Pf1N1B4_274 0.48 +0.3
334,112 + Pf1N1B4_274 0.48 +1.8
334,112 + Pf1N1B4_274 0.48 +0.2
334,112 + Pf1N1B4_274 0.48 -2.0
334,112 + Pf1N1B4_274 0.48 +0.2
334,112 + Pf1N1B4_274 0.48 -0.1
334,113 - Pf1N1B4_274 0.49 -0.1
334,113 - Pf1N1B4_274 0.49 -1.1
334,430 + Pf1N1B4_274 0.80 +2.6
334,430 + Pf1N1B4_274 0.80 -0.2
334,430 + Pf1N1B4_274 0.80 +0.9
334,430 + Pf1N1B4_274 0.80 -1.2
334,431 - Pf1N1B4_274 0.81 +3.4
334,431 - +0.8
334,431 - -1.2
334,431 - Pf1N1B4_274 0.81 -1.3
334,433 + Pf1N1B4_274 0.81 +0.2
334,498 + -0.8
334,498 + Pf1N1B4_274 0.87 +0.8
334,498 + Pf1N1B4_274 0.87 -0.8
334,499 - Pf1N1B4_274 0.87 -0.8
334,499 - Pf1N1B4_274 0.87 -0.4
334,499 - Pf1N1B4_274 0.87 -1.2
334,499 - Pf1N1B4_274 0.87 -0.6
334,552 + +1.8

Or see this region's nucleotide sequence