Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2647

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2645 and Pf1N1B4_2646 are separated by 31 nucleotidesPf1N1B4_2646 and Pf1N1B4_2647 overlap by 4 nucleotidesPf1N1B4_2647 and Pf1N1B4_2648 are separated by 117 nucleotidesPf1N1B4_2648 and Pf1N1B4_2649 overlap by 4 nucleotides Pf1N1B4_2645 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 2,983,999 to 2,984,871 _2645 Pf1N1B4_2646 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,984,903 to 2,985,637 _2646 Pf1N1B4_2647 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,985,634 to 2,986,098 _2647 Pf1N1B4_2648 - Ribonucleotide reductase transcriptional regulator NrdR, at 2,986,216 to 2,986,680 _2648 Pf1N1B4_2649 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 2,986,677 to 2,987,810 _2649 Position (kb) 2985 2986 2987Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2984.708 kb on + strand, within Pf1N1B4_2645at 2984.709 kb on - strand, within Pf1N1B4_2645at 2984.709 kb on - strand, within Pf1N1B4_2645at 2984.853 kb on - strandat 2984.853 kb on - strandat 2984.883 kb on + strandat 2984.883 kb on + strandat 2984.883 kb on + strandat 2984.884 kb on - strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.904 kb on + strandat 2984.905 kb on - strandat 2984.905 kb on - strandat 2984.905 kb on - strandat 2984.905 kb on - strandat 2984.905 kb on - strandat 2984.906 kb on + strandat 2984.906 kb on + strandat 2984.906 kb on + strandat 2984.906 kb on + strandat 2984.907 kb on - strandat 2984.907 kb on - strandat 2984.907 kb on - strandat 2984.907 kb on - strandat 2984.907 kb on - strandat 2985.001 kb on - strand, within Pf1N1B4_2646at 2985.078 kb on + strand, within Pf1N1B4_2646at 2985.079 kb on - strand, within Pf1N1B4_2646at 2985.079 kb on - strand, within Pf1N1B4_2646at 2985.079 kb on - strand, within Pf1N1B4_2646at 2985.178 kb on - strand, within Pf1N1B4_2646at 2985.178 kb on - strand, within Pf1N1B4_2646at 2985.178 kb on - strand, within Pf1N1B4_2646at 2985.178 kb on - strand, within Pf1N1B4_2646at 2985.391 kb on + strand, within Pf1N1B4_2646at 2985.424 kb on - strand, within Pf1N1B4_2646at 2985.520 kb on + strand, within Pf1N1B4_2646at 2985.575 kb on + strandat 2985.575 kb on + strandat 2985.575 kb on + strandat 2985.576 kb on - strandat 2985.576 kb on - strandat 2985.576 kb on - strandat 2985.576 kb on - strandat 2985.577 kb on + strandat 2985.577 kb on + strandat 2985.578 kb on - strandat 2985.578 kb on - strandat 2985.578 kb on - strandat 2985.578 kb on - strandat 2985.844 kb on + strand, within Pf1N1B4_2647at 2986.125 kb on - strandat 2986.140 kb on - strandat 2986.154 kb on - strandat 2986.154 kb on - strandat 2986.182 kb on - strandat 2986.585 kb on + strand, within Pf1N1B4_2648at 2986.716 kb on + strandat 2986.716 kb on + strandat 2986.716 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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2,984,708 + Pf1N1B4_2645 0.81 +0.9
2,984,709 - Pf1N1B4_2645 0.81 -0.1
2,984,709 - Pf1N1B4_2645 0.81 -0.0
2,984,853 - +1.0
2,984,853 - +1.0
2,984,883 + -1.5
2,984,883 + +0.1
2,984,883 + -1.5
2,984,884 - +0.1
2,984,904 + +1.8
2,984,904 + +1.9
2,984,904 + +0.3
2,984,904 + +0.8
2,984,904 + -0.9
2,984,904 + +0.1
2,984,904 + +1.4
2,984,904 + +0.1
2,984,905 - -0.6
2,984,905 - -1.2
2,984,905 - -0.2
2,984,905 - -0.8
2,984,905 - +1.1
2,984,906 + -0.9
2,984,906 + +1.3
2,984,906 + +0.1
2,984,906 + +2.1
2,984,907 - -0.9
2,984,907 - -0.0
2,984,907 - +1.1
2,984,907 - +0.2
2,984,907 - +1.1
2,985,001 - Pf1N1B4_2646 0.13 +0.6
2,985,078 + Pf1N1B4_2646 0.24 +0.6
2,985,079 - Pf1N1B4_2646 0.24 +0.1
2,985,079 - Pf1N1B4_2646 0.24 -0.5
2,985,079 - Pf1N1B4_2646 0.24 -1.7
2,985,178 - Pf1N1B4_2646 0.37 -1.5
2,985,178 - Pf1N1B4_2646 0.37 +0.4
2,985,178 - Pf1N1B4_2646 0.37 +0.1
2,985,178 - Pf1N1B4_2646 0.37 -0.8
2,985,391 + Pf1N1B4_2646 0.66 -0.3
2,985,424 - Pf1N1B4_2646 0.71 +0.3
2,985,520 + Pf1N1B4_2646 0.84 +0.8
2,985,575 + +0.1
2,985,575 + -0.3
2,985,575 + +1.9
2,985,576 - +1.1
2,985,576 - +0.9
2,985,576 - +0.8
2,985,576 - +0.2
2,985,577 + +4.3
2,985,577 + +0.7
2,985,578 - +0.4
2,985,578 - +1.1
2,985,578 - +0.5
2,985,578 - +1.4
2,985,844 + Pf1N1B4_2647 0.45 -1.7
2,986,125 - -2.0
2,986,140 - +0.8
2,986,154 - -1.4
2,986,154 - -0.2
2,986,182 - +0.8
2,986,585 + Pf1N1B4_2648 0.79 +1.4
2,986,716 + -1.2
2,986,716 + +1.3
2,986,716 + -0.2

Or see this region's nucleotide sequence