Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2645 and Pf1N1B4_2646 are separated by 31 nucleotides Pf1N1B4_2646 and Pf1N1B4_2647 overlap by 4 nucleotides Pf1N1B4_2647 and Pf1N1B4_2648 are separated by 117 nucleotides Pf1N1B4_2648 and Pf1N1B4_2649 overlap by 4 nucleotides
Pf1N1B4_2645 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 2,983,999 to 2,984,871
_2645
Pf1N1B4_2646 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,984,903 to 2,985,637
_2646
Pf1N1B4_2647 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,985,634 to 2,986,098
_2647
Pf1N1B4_2648 - Ribonucleotide reductase transcriptional regulator NrdR, at 2,986,216 to 2,986,680
_2648
Pf1N1B4_2649 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 2,986,677 to 2,987,810
_2649
Position (kb)
2985
2986
2987 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2984.708 kb on + strand, within Pf1N1B4_2645 at 2984.709 kb on - strand, within Pf1N1B4_2645 at 2984.709 kb on - strand, within Pf1N1B4_2645 at 2984.853 kb on - strand at 2984.853 kb on - strand at 2984.883 kb on + strand at 2984.883 kb on + strand at 2984.883 kb on + strand at 2984.884 kb on - strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.904 kb on + strand at 2984.905 kb on - strand at 2984.905 kb on - strand at 2984.905 kb on - strand at 2984.905 kb on - strand at 2984.905 kb on - strand at 2984.906 kb on + strand at 2984.906 kb on + strand at 2984.906 kb on + strand at 2984.906 kb on + strand at 2984.907 kb on - strand at 2984.907 kb on - strand at 2984.907 kb on - strand at 2984.907 kb on - strand at 2984.907 kb on - strand at 2985.001 kb on - strand, within Pf1N1B4_2646 at 2985.078 kb on + strand, within Pf1N1B4_2646 at 2985.079 kb on - strand, within Pf1N1B4_2646 at 2985.079 kb on - strand, within Pf1N1B4_2646 at 2985.079 kb on - strand, within Pf1N1B4_2646 at 2985.178 kb on - strand, within Pf1N1B4_2646 at 2985.178 kb on - strand, within Pf1N1B4_2646 at 2985.178 kb on - strand, within Pf1N1B4_2646 at 2985.178 kb on - strand, within Pf1N1B4_2646 at 2985.391 kb on + strand, within Pf1N1B4_2646 at 2985.424 kb on - strand, within Pf1N1B4_2646 at 2985.520 kb on + strand, within Pf1N1B4_2646 at 2985.575 kb on + strand at 2985.575 kb on + strand at 2985.575 kb on + strand at 2985.576 kb on - strand at 2985.576 kb on - strand at 2985.576 kb on - strand at 2985.576 kb on - strand at 2985.577 kb on + strand at 2985.577 kb on + strand at 2985.578 kb on - strand at 2985.578 kb on - strand at 2985.578 kb on - strand at 2985.578 kb on - strand at 2985.844 kb on + strand, within Pf1N1B4_2647 at 2986.125 kb on - strand at 2986.140 kb on - strand at 2986.154 kb on - strand at 2986.154 kb on - strand at 2986.182 kb on - strand at 2986.585 kb on + strand, within Pf1N1B4_2648 at 2986.716 kb on + strand at 2986.716 kb on + strand at 2986.716 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,984,708 + Pf1N1B4_2645 0.81 +0.9 2,984,709 - Pf1N1B4_2645 0.81 -0.1 2,984,709 - Pf1N1B4_2645 0.81 -0.0 2,984,853 - +1.0 2,984,853 - +1.0 2,984,883 + -1.5 2,984,883 + +0.1 2,984,883 + -1.5 2,984,884 - +0.1 2,984,904 + +1.8 2,984,904 + +1.9 2,984,904 + +0.3 2,984,904 + +0.8 2,984,904 + -0.9 2,984,904 + +0.1 2,984,904 + +1.4 2,984,904 + +0.1 2,984,905 - -0.6 2,984,905 - -1.2 2,984,905 - -0.2 2,984,905 - -0.8 2,984,905 - +1.1 2,984,906 + -0.9 2,984,906 + +1.3 2,984,906 + +0.1 2,984,906 + +2.1 2,984,907 - -0.9 2,984,907 - -0.0 2,984,907 - +1.1 2,984,907 - +0.2 2,984,907 - +1.1 2,985,001 - Pf1N1B4_2646 0.13 +0.6 2,985,078 + Pf1N1B4_2646 0.24 +0.6 2,985,079 - Pf1N1B4_2646 0.24 +0.1 2,985,079 - Pf1N1B4_2646 0.24 -0.5 2,985,079 - Pf1N1B4_2646 0.24 -1.7 2,985,178 - Pf1N1B4_2646 0.37 -1.5 2,985,178 - Pf1N1B4_2646 0.37 +0.4 2,985,178 - Pf1N1B4_2646 0.37 +0.1 2,985,178 - Pf1N1B4_2646 0.37 -0.8 2,985,391 + Pf1N1B4_2646 0.66 -0.3 2,985,424 - Pf1N1B4_2646 0.71 +0.3 2,985,520 + Pf1N1B4_2646 0.84 +0.8 2,985,575 + +0.1 2,985,575 + -0.3 2,985,575 + +1.9 2,985,576 - +1.1 2,985,576 - +0.9 2,985,576 - +0.8 2,985,576 - +0.2 2,985,577 + +4.3 2,985,577 + +0.7 2,985,578 - +0.4 2,985,578 - +1.1 2,985,578 - +0.5 2,985,578 - +1.4 2,985,844 + Pf1N1B4_2647 0.45 -1.7 2,986,125 - -2.0 2,986,140 - +0.8 2,986,154 - -1.4 2,986,154 - -0.2 2,986,182 - +0.8 2,986,585 + Pf1N1B4_2648 0.79 +1.4 2,986,716 + -1.2 2,986,716 + +1.3 2,986,716 + -0.2
Or see this region's nucleotide sequence