Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2433 and Pf1N1B4_2434 are separated by 32 nucleotides Pf1N1B4_2434 and Pf1N1B4_2435 are separated by 40 nucleotides Pf1N1B4_2435 and Pf1N1B4_2436 are separated by 306 nucleotides Pf1N1B4_2436 and Pf1N1B4_2437 are separated by 245 nucleotides
Pf1N1B4_2433 - FIG00953108: hypothetical protein, at 2,758,487 to 2,758,903
_2433
Pf1N1B4_2434 - putative DNA binding protein, at 2,758,936 to 2,759,202
_2434
Pf1N1B4_2435 - hypothetical protein, at 2,759,243 to 2,759,395
_2435
Pf1N1B4_2436 - FIG00953563: hypothetical protein, at 2,759,702 to 2,760,103
_2436
Pf1N1B4_2437 - Microsomal dipeptidase (EC 3.4.13.19), at 2,760,349 to 2,761,326
_2437
Position (kb)
2759
2760
2761 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.938 kb on - strand at 2758.938 kb on - strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2759.076 kb on + strand, within Pf1N1B4_2434 at 2759.077 kb on - strand, within Pf1N1B4_2434 at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.247 kb on + strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.281 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.329 kb on + strand, within Pf1N1B4_2435 at 2759.352 kb on + strand, within Pf1N1B4_2435 at 2759.353 kb on - strand, within Pf1N1B4_2435 at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.385 kb on - strand at 2759.503 kb on + strand at 2759.590 kb on + strand at 2759.622 kb on + strand at 2759.659 kb on + strand at 2759.660 kb on - strand at 2759.670 kb on - strand at 2759.911 kb on - strand, within Pf1N1B4_2436 at 2759.943 kb on + strand, within Pf1N1B4_2436 at 2759.944 kb on - strand, within Pf1N1B4_2436 at 2759.944 kb on - strand, within Pf1N1B4_2436 at 2759.983 kb on - strand, within Pf1N1B4_2436 at 2759.983 kb on - strand, within Pf1N1B4_2436 at 2759.983 kb on - strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.059 kb on + strand, within Pf1N1B4_2436 at 2760.060 kb on - strand, within Pf1N1B4_2436 at 2760.060 kb on - strand, within Pf1N1B4_2436 at 2760.060 kb on - strand, within Pf1N1B4_2436 at 2760.139 kb on + strand at 2760.186 kb on + strand at 2760.186 kb on + strand at 2760.187 kb on - strand at 2760.286 kb on + strand at 2760.286 kb on + strand at 2760.286 kb on + strand at 2760.286 kb on + strand at 2760.287 kb on - strand at 2760.335 kb on + strand at 2760.335 kb on + strand at 2760.400 kb on - strand at 2760.658 kb on + strand, within Pf1N1B4_2437 at 2760.706 kb on + strand, within Pf1N1B4_2437 at 2760.706 kb on + strand, within Pf1N1B4_2437 at 2760.706 kb on + strand, within Pf1N1B4_2437 at 2760.706 kb on + strand, within Pf1N1B4_2437 at 2760.707 kb on - strand, within Pf1N1B4_2437 at 2760.707 kb on - strand, within Pf1N1B4_2437 at 2760.707 kb on - strand, within Pf1N1B4_2437 at 2760.707 kb on - strand, within Pf1N1B4_2437 at 2760.740 kb on - strand, within Pf1N1B4_2437 at 2760.881 kb on - strand, within Pf1N1B4_2437 at 2760.881 kb on - strand, within Pf1N1B4_2437 at 2760.881 kb on - strand, within Pf1N1B4_2437 at 2761.048 kb on + strand, within Pf1N1B4_2437 at 2761.048 kb on + strand, within Pf1N1B4_2437 at 2761.048 kb on + strand, within Pf1N1B4_2437
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,758,937 + -0.4 2,758,937 + +0.8 2,758,937 + -1.7 2,758,938 - -1.4 2,758,938 - -0.1 2,758,945 + -0.6 2,758,945 + -1.0 2,758,945 + -0.4 2,759,076 + Pf1N1B4_2434 0.52 -0.4 2,759,077 - Pf1N1B4_2434 0.53 -1.6 2,759,180 + -0.6 2,759,180 + +2.4 2,759,180 + +0.2 2,759,181 - +1.0 2,759,181 - +0.7 2,759,181 - -2.0 2,759,181 - -1.1 2,759,181 - +1.2 2,759,247 + -2.0 2,759,248 - -0.4 2,759,248 - +0.3 2,759,248 - +0.2 2,759,248 - +0.6 2,759,281 + Pf1N1B4_2435 0.25 +0.2 2,759,317 + Pf1N1B4_2435 0.48 -0.1 2,759,317 + Pf1N1B4_2435 0.48 +1.9 2,759,329 + Pf1N1B4_2435 0.56 +1.1 2,759,352 + Pf1N1B4_2435 0.71 -0.8 2,759,353 - Pf1N1B4_2435 0.72 -0.1 2,759,384 + -0.0 2,759,384 + +0.5 2,759,384 + +1.0 2,759,384 + -1.8 2,759,384 + +0.7 2,759,384 + +0.4 2,759,385 - +1.7 2,759,503 + +0.3 2,759,590 + +2.6 2,759,622 + -0.4 2,759,659 + +1.2 2,759,660 - -0.8 2,759,670 - +0.1 2,759,911 - Pf1N1B4_2436 0.52 +0.9 2,759,943 + Pf1N1B4_2436 0.60 +2.0 2,759,944 - Pf1N1B4_2436 0.60 +2.0 2,759,944 - Pf1N1B4_2436 0.60 +2.1 2,759,983 - Pf1N1B4_2436 0.70 +0.2 2,759,983 - Pf1N1B4_2436 0.70 +1.3 2,759,983 - Pf1N1B4_2436 0.70 +2.2 2,760,059 + Pf1N1B4_2436 0.89 +2.0 2,760,059 + Pf1N1B4_2436 0.89 +2.4 2,760,059 + Pf1N1B4_2436 0.89 +2.0 2,760,059 + Pf1N1B4_2436 0.89 +0.5 2,760,059 + Pf1N1B4_2436 0.89 +1.5 2,760,059 + Pf1N1B4_2436 0.89 +1.6 2,760,059 + Pf1N1B4_2436 0.89 +0.5 2,760,060 - Pf1N1B4_2436 0.89 +1.4 2,760,060 - Pf1N1B4_2436 0.89 +0.2 2,760,060 - Pf1N1B4_2436 0.89 +1.0 2,760,139 + +1.7 2,760,186 + +3.8 2,760,186 + +2.5 2,760,187 - -0.0 2,760,286 + -2.0 2,760,286 + +0.5 2,760,286 + +0.6 2,760,286 + +1.9 2,760,287 - +0.2 2,760,335 + -0.3 2,760,335 + +0.2 2,760,400 - -0.1 2,760,658 + Pf1N1B4_2437 0.32 +1.2 2,760,706 + Pf1N1B4_2437 0.37 +0.6 2,760,706 + Pf1N1B4_2437 0.37 +0.6 2,760,706 + Pf1N1B4_2437 0.37 -0.7 2,760,706 + Pf1N1B4_2437 0.37 -0.4 2,760,707 - Pf1N1B4_2437 0.37 -0.8 2,760,707 - Pf1N1B4_2437 0.37 -0.8 2,760,707 - Pf1N1B4_2437 0.37 -2.1 2,760,707 - Pf1N1B4_2437 0.37 +0.4 2,760,740 - Pf1N1B4_2437 0.40 +1.2 2,760,881 - Pf1N1B4_2437 0.54 -0.2 2,760,881 - Pf1N1B4_2437 0.54 -0.2 2,760,881 - Pf1N1B4_2437 0.54 +0.2 2,761,048 + Pf1N1B4_2437 0.71 +2.5 2,761,048 + Pf1N1B4_2437 0.71 -0.2 2,761,048 + Pf1N1B4_2437 0.71 -1.0
Or see this region's nucleotide sequence