Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2389 and Pf1N1B4_2390 are separated by 185 nucleotides Pf1N1B4_2390 and Pf1N1B4_2391 are separated by 116 nucleotides Pf1N1B4_2391 and Pf1N1B4_2392 are separated by 128 nucleotides Pf1N1B4_2392 and Pf1N1B4_2393 are separated by 42 nucleotides
Pf1N1B4_2389 - Integral membrane protein, at 2,707,335 to 2,708,168
_2389
Pf1N1B4_2390 - probable membrane protein NMA1176, at 2,708,354 to 2,708,602
_2390
Pf1N1B4_2391 - Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-), at 2,708,719 to 2,709,891
_2391
Pf1N1B4_2392 - Cytochrome c556, at 2,710,020 to 2,710,469
_2392
Pf1N1B4_2393 - Protein yqjC precursor, at 2,710,512 to 2,710,907
_2393
Position (kb)
2708
2709
2710 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2707.800 kb on + strand, within Pf1N1B4_2389 at 2707.800 kb on + strand, within Pf1N1B4_2389 at 2707.800 kb on + strand, within Pf1N1B4_2389 at 2707.801 kb on - strand, within Pf1N1B4_2389 at 2707.801 kb on - strand, within Pf1N1B4_2389 at 2707.851 kb on + strand, within Pf1N1B4_2389 at 2707.851 kb on + strand, within Pf1N1B4_2389 at 2707.978 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.990 kb on - strand, within Pf1N1B4_2389 at 2708.088 kb on + strand at 2708.089 kb on - strand at 2708.122 kb on + strand at 2708.166 kb on + strand at 2708.194 kb on + strand at 2708.196 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.265 kb on - strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.275 kb on - strand at 2708.309 kb on - strand at 2708.516 kb on + strand, within Pf1N1B4_2390 at 2708.516 kb on + strand, within Pf1N1B4_2390 at 2708.516 kb on + strand, within Pf1N1B4_2390 at 2708.517 kb on - strand, within Pf1N1B4_2390 at 2708.550 kb on + strand, within Pf1N1B4_2390 at 2708.550 kb on + strand, within Pf1N1B4_2390 at 2708.550 kb on + strand, within Pf1N1B4_2390 at 2708.551 kb on - strand, within Pf1N1B4_2390 at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.640 kb on - strand at 2708.720 kb on + strand at 2708.720 kb on + strand at 2708.721 kb on - strand at 2708.721 kb on - strand at 2708.721 kb on - strand at 2708.721 kb on - strand at 2708.735 kb on - strand at 2708.735 kb on - strand at 2708.735 kb on - strand at 2708.883 kb on + strand, within Pf1N1B4_2391 at 2708.989 kb on - strand, within Pf1N1B4_2391 at 2708.990 kb on + strand, within Pf1N1B4_2391 at 2708.990 kb on + strand, within Pf1N1B4_2391 at 2708.991 kb on - strand, within Pf1N1B4_2391 at 2709.065 kb on + strand, within Pf1N1B4_2391 at 2709.065 kb on + strand at 2709.066 kb on - strand at 2709.066 kb on - strand, within Pf1N1B4_2391 at 2709.066 kb on - strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.430 kb on + strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.578 kb on + strand, within Pf1N1B4_2391 at 2709.579 kb on - strand, within Pf1N1B4_2391 at 2709.579 kb on - strand, within Pf1N1B4_2391 at 2709.687 kb on + strand, within Pf1N1B4_2391 at 2709.770 kb on + strand, within Pf1N1B4_2391 at 2709.770 kb on + strand, within Pf1N1B4_2391 at 2709.771 kb on - strand, within Pf1N1B4_2391 at 2709.896 kb on + strand at 2709.896 kb on + strand at 2709.897 kb on - strand at 2709.897 kb on - strand at 2709.897 kb on - strand at 2709.950 kb on + strand at 2709.950 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.954 kb on - strand at 2709.954 kb on - strand at 2710.028 kb on + strand at 2710.029 kb on - strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.176 kb on - strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.387 kb on - strand, within Pf1N1B4_2392 at 2710.387 kb on - strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.517 kb on + strand at 2710.517 kb on + strand at 2710.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,707,800 + Pf1N1B4_2389 0.56 +0.6 2,707,800 + Pf1N1B4_2389 0.56 -0.3 2,707,800 + Pf1N1B4_2389 0.56 +3.2 2,707,801 - Pf1N1B4_2389 0.56 +0.2 2,707,801 - Pf1N1B4_2389 0.56 +0.5 2,707,851 + Pf1N1B4_2389 0.62 -1.6 2,707,851 + Pf1N1B4_2389 0.62 -1.6 2,707,978 + Pf1N1B4_2389 0.77 -1.8 2,707,989 + Pf1N1B4_2389 0.78 +0.0 2,707,989 + Pf1N1B4_2389 0.78 +0.2 2,707,989 + Pf1N1B4_2389 0.78 +0.1 2,707,989 + Pf1N1B4_2389 0.78 +0.4 2,707,989 + Pf1N1B4_2389 0.78 +1.2 2,707,989 + Pf1N1B4_2389 0.78 -0.2 2,707,989 + Pf1N1B4_2389 0.78 -1.5 2,707,990 - Pf1N1B4_2389 0.79 +0.4 2,708,088 + +0.0 2,708,089 - +2.2 2,708,122 + -0.8 2,708,166 + +0.7 2,708,194 + -0.9 2,708,196 + -0.4 2,708,264 + -0.4 2,708,264 + +1.2 2,708,264 + +0.6 2,708,264 + +0.3 2,708,264 + -1.8 2,708,264 + +0.4 2,708,264 + +0.8 2,708,265 - -0.0 2,708,274 + -0.4 2,708,274 + -0.4 2,708,274 + +2.8 2,708,274 + +1.6 2,708,274 + -0.8 2,708,275 - +0.5 2,708,309 - +1.2 2,708,516 + Pf1N1B4_2390 0.65 -0.4 2,708,516 + Pf1N1B4_2390 0.65 -0.6 2,708,516 + Pf1N1B4_2390 0.65 +0.5 2,708,517 - Pf1N1B4_2390 0.65 -0.4 2,708,550 + Pf1N1B4_2390 0.79 -1.4 2,708,550 + Pf1N1B4_2390 0.79 +0.3 2,708,550 + Pf1N1B4_2390 0.79 +1.1 2,708,551 - Pf1N1B4_2390 0.79 -0.3 2,708,639 + +1.7 2,708,639 + -0.1 2,708,639 + -0.8 2,708,639 + -0.5 2,708,639 + +1.8 2,708,639 + -2.0 2,708,639 + -1.1 2,708,639 + -1.4 2,708,639 + +1.8 2,708,640 - +0.2 2,708,720 + -1.4 2,708,720 + +1.7 2,708,721 - +0.1 2,708,721 - -0.4 2,708,721 - -2.0 2,708,721 - +0.5 2,708,735 - -0.8 2,708,735 - -0.0 2,708,735 - -0.1 2,708,883 + Pf1N1B4_2391 0.14 -0.1 2,708,989 - Pf1N1B4_2391 0.23 -0.4 2,708,990 + Pf1N1B4_2391 0.23 -0.5 2,708,990 + Pf1N1B4_2391 0.23 -1.6 2,708,991 - Pf1N1B4_2391 0.23 +0.4 2,709,065 + Pf1N1B4_2391 0.29 +1.2 2,709,065 + +0.6 2,709,066 - -0.4 2,709,066 - Pf1N1B4_2391 0.30 -2.1 2,709,066 - Pf1N1B4_2391 0.30 -0.8 2,709,425 + Pf1N1B4_2391 0.60 -0.5 2,709,425 + Pf1N1B4_2391 0.60 -0.8 2,709,425 + Pf1N1B4_2391 0.60 +0.6 2,709,429 + Pf1N1B4_2391 0.61 +0.2 2,709,429 + Pf1N1B4_2391 0.61 -1.0 2,709,429 + Pf1N1B4_2391 0.61 -2.4 2,709,429 + Pf1N1B4_2391 0.61 -2.7 2,709,429 + Pf1N1B4_2391 0.61 -1.0 2,709,429 + Pf1N1B4_2391 0.61 +0.1 2,709,429 + Pf1N1B4_2391 0.61 +1.2 2,709,429 + Pf1N1B4_2391 0.61 -1.4 2,709,429 + Pf1N1B4_2391 0.61 -2.0 2,709,429 + Pf1N1B4_2391 0.61 +0.2 2,709,429 + +1.2 2,709,429 + Pf1N1B4_2391 0.61 +0.6 2,709,429 + Pf1N1B4_2391 0.61 -1.4 2,709,429 + Pf1N1B4_2391 0.61 -0.8 2,709,429 + Pf1N1B4_2391 0.61 -1.1 2,709,429 + Pf1N1B4_2391 0.61 -0.0 2,709,429 + Pf1N1B4_2391 0.61 -1.1 2,709,429 + Pf1N1B4_2391 0.61 -1.4 2,709,429 + Pf1N1B4_2391 0.61 -1.4 2,709,429 + Pf1N1B4_2391 0.61 -1.4 2,709,429 + Pf1N1B4_2391 0.61 -2.6 2,709,430 + Pf1N1B4_2391 0.61 -1.3 2,709,430 - Pf1N1B4_2391 0.61 +0.1 2,709,430 - Pf1N1B4_2391 0.61 -1.6 2,709,430 - Pf1N1B4_2391 0.61 -1.6 2,709,430 - Pf1N1B4_2391 0.61 +1.2 2,709,430 - Pf1N1B4_2391 0.61 -1.9 2,709,430 - Pf1N1B4_2391 0.61 -2.0 2,709,430 - Pf1N1B4_2391 0.61 -1.6 2,709,430 - Pf1N1B4_2391 0.61 -1.0 2,709,430 - Pf1N1B4_2391 0.61 -1.7 2,709,430 - Pf1N1B4_2391 0.61 -1.1 2,709,430 - Pf1N1B4_2391 0.61 +0.5 2,709,430 - Pf1N1B4_2391 0.61 -1.1 2,709,430 - Pf1N1B4_2391 0.61 -1.2 2,709,430 - Pf1N1B4_2391 0.61 -0.2 2,709,430 - Pf1N1B4_2391 0.61 +0.3 2,709,470 + Pf1N1B4_2391 0.64 +0.2 2,709,470 + Pf1N1B4_2391 0.64 -0.1 2,709,470 + Pf1N1B4_2391 0.64 -0.0 2,709,470 + Pf1N1B4_2391 0.64 -0.6 2,709,471 - Pf1N1B4_2391 0.64 -0.1 2,709,471 - Pf1N1B4_2391 0.64 -1.8 2,709,471 - Pf1N1B4_2391 0.64 -0.7 2,709,471 - Pf1N1B4_2391 0.64 -0.1 2,709,527 + Pf1N1B4_2391 0.69 -1.8 2,709,527 + Pf1N1B4_2391 0.69 -2.5 2,709,527 + Pf1N1B4_2391 0.69 +0.9 2,709,528 - Pf1N1B4_2391 0.69 +0.5 2,709,528 - Pf1N1B4_2391 0.69 -0.2 2,709,528 - Pf1N1B4_2391 0.69 -1.1 2,709,528 - Pf1N1B4_2391 0.69 -0.4 2,709,528 - Pf1N1B4_2391 0.69 -0.8 2,709,528 - Pf1N1B4_2391 0.69 -0.4 2,709,528 - Pf1N1B4_2391 0.69 -1.6 2,709,528 - Pf1N1B4_2391 0.69 -1.6 2,709,578 + Pf1N1B4_2391 0.73 -2.1 2,709,579 - Pf1N1B4_2391 0.73 +0.9 2,709,579 - Pf1N1B4_2391 0.73 -1.5 2,709,687 + Pf1N1B4_2391 0.83 -2.1 2,709,770 + Pf1N1B4_2391 0.90 -2.3 2,709,770 + Pf1N1B4_2391 0.90 -0.7 2,709,771 - Pf1N1B4_2391 0.90 +0.1 2,709,896 + -1.7 2,709,896 + -0.5 2,709,897 - -0.0 2,709,897 - +0.2 2,709,897 - +0.4 2,709,950 + +0.2 2,709,950 + -1.4 2,709,953 + -1.4 2,709,953 + -0.4 2,709,953 + -2.4 2,709,953 + +0.2 2,709,954 - +0.2 2,709,954 - -0.7 2,710,028 + +0.9 2,710,029 - +1.7 2,710,045 + -1.5 2,710,045 + -0.4 2,710,045 + -1.3 2,710,045 + +0.7 2,710,045 + -0.0 2,710,045 + -1.1 2,710,045 + +0.4 2,710,045 + -2.1 2,710,045 + -2.0 2,710,045 + -2.1 2,710,045 + +1.2 2,710,045 + -0.6 2,710,045 + -1.4 2,710,046 - +1.3 2,710,046 - +0.2 2,710,046 - +0.1 2,710,046 - +0.4 2,710,175 + Pf1N1B4_2392 0.34 +1.7 2,710,175 + Pf1N1B4_2392 0.34 -0.9 2,710,175 + Pf1N1B4_2392 0.34 -0.2 2,710,176 - Pf1N1B4_2392 0.35 +2.0 2,710,386 + Pf1N1B4_2392 0.81 +0.6 2,710,386 + Pf1N1B4_2392 0.81 +0.5 2,710,387 - Pf1N1B4_2392 0.82 +1.8 2,710,387 - +0.2 2,710,513 + +1.6 2,710,513 + -1.1 2,710,513 + -2.1 2,710,513 + +1.2 2,710,513 + -1.1 2,710,514 - +0.9 2,710,514 - +1.5 2,710,514 - +0.6 2,710,517 + +1.5 2,710,517 + +0.5 2,710,517 + -1.1
Or see this region's nucleotide sequence