Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2023 and Pf1N1B4_2024 are separated by 175 nucleotides Pf1N1B4_2024 and Pf1N1B4_2025 are separated by 538 nucleotides
Pf1N1B4_2023 - L-asparaginase (EC 3.5.1.1) (from data) , at 2,308,645 to 2,309,649
_2023
Pf1N1B4_2024 - sodium/alanine transporter, at 2,309,825 to 2,311,276
_2024
Pf1N1B4_2025 - Aspartate ammonia-lyase (EC 4.3.1.1), at 2,311,815 to 2,313,239
_2025
Position (kb)
2309
2310
2311
2312 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2309.037 kb on + strand, within Pf1N1B4_2023 at 2309.038 kb on - strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.339 kb on + strand, within Pf1N1B4_2023 at 2309.340 kb on - strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.753 kb on + strand at 2309.754 kb on - strand at 2310.126 kb on - strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.199 kb on - strand at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.208 kb on - strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.662 kb on + strand, within Pf1N1B4_2024 at 2310.747 kb on + strand, within Pf1N1B4_2024 at 2310.747 kb on + strand, within Pf1N1B4_2024 at 2310.747 kb on + strand, within Pf1N1B4_2024 at 2310.748 kb on - strand, within Pf1N1B4_2024 at 2310.891 kb on + strand, within Pf1N1B4_2024 at 2310.892 kb on - strand, within Pf1N1B4_2024 at 2311.206 kb on + strand at 2311.206 kb on + strand at 2311.206 kb on + strand at 2311.207 kb on - strand at 2311.239 kb on - strand at 2311.281 kb on + strand at 2311.335 kb on - strand at 2311.402 kb on + strand at 2311.402 kb on + strand at 2311.402 kb on + strand at 2311.403 kb on - strand at 2311.403 kb on - strand at 2311.403 kb on - strand at 2311.403 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.471 kb on - strand at 2311.471 kb on - strand at 2311.471 kb on - strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.560 kb on + strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.564 kb on + strand at 2311.564 kb on + strand at 2311.564 kb on + strand at 2311.564 kb on + strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.734 kb on - strand at 2311.734 kb on - strand at 2311.734 kb on - strand at 2311.734 kb on - strand at 2311.767 kb on + strand at 2311.767 kb on + strand at 2311.768 kb on - strand at 2311.768 kb on - strand at 2311.982 kb on - strand, within Pf1N1B4_2025
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,308,931 + Pf1N1B4_2023 0.28 +2.5 2,308,931 + Pf1N1B4_2023 0.28 +1.3 2,309,037 + Pf1N1B4_2023 0.39 +2.0 2,309,038 - Pf1N1B4_2023 0.39 +1.6 2,309,048 + Pf1N1B4_2023 0.40 +0.6 2,309,048 + Pf1N1B4_2023 0.40 +1.5 2,309,048 + Pf1N1B4_2023 0.40 +1.4 2,309,049 - Pf1N1B4_2023 0.40 +2.3 2,309,049 - Pf1N1B4_2023 0.40 +1.8 2,309,049 - Pf1N1B4_2023 0.40 +2.7 2,309,049 - Pf1N1B4_2023 0.40 +1.4 2,309,177 + Pf1N1B4_2023 0.53 +1.0 2,309,177 + Pf1N1B4_2023 0.53 -0.3 2,309,177 + Pf1N1B4_2023 0.53 +1.5 2,309,178 - Pf1N1B4_2023 0.53 +0.2 2,309,178 - Pf1N1B4_2023 0.53 -1.9 2,309,178 - Pf1N1B4_2023 0.53 +1.3 2,309,178 - Pf1N1B4_2023 0.53 +0.8 2,309,178 - Pf1N1B4_2023 0.53 +1.4 2,309,178 - Pf1N1B4_2023 0.53 +0.9 2,309,339 + Pf1N1B4_2023 0.69 +0.7 2,309,340 - Pf1N1B4_2023 0.69 +0.4 2,309,522 + Pf1N1B4_2023 0.87 +0.5 2,309,522 + Pf1N1B4_2023 0.87 -1.0 2,309,522 + Pf1N1B4_2023 0.87 +0.3 2,309,523 - Pf1N1B4_2023 0.87 +1.4 2,309,523 - Pf1N1B4_2023 0.87 +0.6 2,309,604 - +1.3 2,309,604 - +0.3 2,309,604 - +0.6 2,309,604 - +0.5 2,309,604 - +0.4 2,309,604 - +1.1 2,309,753 + -1.7 2,309,754 - +1.3 2,310,126 - Pf1N1B4_2024 0.21 +1.5 2,310,198 + Pf1N1B4_2024 0.26 +0.5 2,310,198 + Pf1N1B4_2024 0.26 +1.0 2,310,198 + Pf1N1B4_2024 0.26 -0.3 2,310,199 - +2.8 2,310,199 - Pf1N1B4_2024 0.26 -0.3 2,310,199 - Pf1N1B4_2024 0.26 +1.6 2,310,201 + Pf1N1B4_2024 0.26 +2.1 2,310,201 + Pf1N1B4_2024 0.26 +2.7 2,310,201 + Pf1N1B4_2024 0.26 -1.6 2,310,201 + Pf1N1B4_2024 0.26 +1.1 2,310,201 + Pf1N1B4_2024 0.26 +0.0 2,310,201 + Pf1N1B4_2024 0.26 +0.9 2,310,202 - Pf1N1B4_2024 0.26 -0.3 2,310,202 - Pf1N1B4_2024 0.26 +1.3 2,310,202 - Pf1N1B4_2024 0.26 -0.2 2,310,202 - Pf1N1B4_2024 0.26 +1.1 2,310,202 - Pf1N1B4_2024 0.26 +0.3 2,310,202 - Pf1N1B4_2024 0.26 +0.5 2,310,202 - Pf1N1B4_2024 0.26 +1.5 2,310,202 - Pf1N1B4_2024 0.26 +0.6 2,310,202 - Pf1N1B4_2024 0.26 +0.7 2,310,207 + Pf1N1B4_2024 0.26 +0.5 2,310,207 + Pf1N1B4_2024 0.26 +1.8 2,310,208 - Pf1N1B4_2024 0.26 +2.1 2,310,333 + Pf1N1B4_2024 0.35 -0.9 2,310,333 + Pf1N1B4_2024 0.35 +1.6 2,310,624 + Pf1N1B4_2024 0.55 -1.4 2,310,624 + Pf1N1B4_2024 0.55 -1.9 2,310,624 + Pf1N1B4_2024 0.55 +0.7 2,310,624 + Pf1N1B4_2024 0.55 +0.4 2,310,624 + Pf1N1B4_2024 0.55 +0.1 2,310,625 - Pf1N1B4_2024 0.55 +0.5 2,310,625 - Pf1N1B4_2024 0.55 -1.6 2,310,625 - Pf1N1B4_2024 0.55 +0.7 2,310,625 - Pf1N1B4_2024 0.55 -0.3 2,310,625 - Pf1N1B4_2024 0.55 -0.6 2,310,625 - Pf1N1B4_2024 0.55 +0.5 2,310,625 - Pf1N1B4_2024 0.55 +1.0 2,310,642 + Pf1N1B4_2024 0.56 +1.0 2,310,642 + Pf1N1B4_2024 0.56 -0.3 2,310,642 + Pf1N1B4_2024 0.56 +3.3 2,310,642 + Pf1N1B4_2024 0.56 -0.7 2,310,642 + Pf1N1B4_2024 0.56 +1.6 2,310,642 + Pf1N1B4_2024 0.56 +0.7 2,310,643 - Pf1N1B4_2024 0.56 +1.3 2,310,643 - Pf1N1B4_2024 0.56 +0.0 2,310,643 - Pf1N1B4_2024 0.56 +0.5 2,310,643 - Pf1N1B4_2024 0.56 +0.3 2,310,662 + Pf1N1B4_2024 0.58 -1.7 2,310,747 + Pf1N1B4_2024 0.63 -0.7 2,310,747 + Pf1N1B4_2024 0.63 +2.0 2,310,747 + Pf1N1B4_2024 0.63 -1.3 2,310,748 - Pf1N1B4_2024 0.64 +2.4 2,310,891 + Pf1N1B4_2024 0.73 -0.7 2,310,892 - Pf1N1B4_2024 0.73 -0.6 2,311,206 + +1.4 2,311,206 + +0.3 2,311,206 + +1.3 2,311,207 - +0.2 2,311,239 - -0.2 2,311,281 + -0.2 2,311,335 - -0.4 2,311,402 + -1.3 2,311,402 + -0.2 2,311,402 + +0.3 2,311,403 - -1.7 2,311,403 - -3.3 2,311,403 - -1.1 2,311,403 - -1.1 2,311,406 - -1.1 2,311,406 - -1.1 2,311,406 - -2.6 2,311,406 - -2.6 2,311,406 - -1.4 2,311,406 - -2.0 2,311,406 - -2.4 2,311,406 - -1.1 2,311,406 - -1.7 2,311,406 - -2.7 2,311,471 - -2.1 2,311,471 - -1.2 2,311,471 - -1.7 2,311,472 + +0.8 2,311,472 + -0.1 2,311,472 + +0.4 2,311,472 + +1.3 2,311,472 + +1.3 2,311,472 + +0.4 2,311,472 + +2.5 2,311,472 + -1.1 2,311,472 + -1.1 2,311,472 + +0.7 2,311,472 + +1.8 2,311,472 + -0.5 2,311,472 + +0.5 2,311,472 + +0.7 2,311,472 + -0.6 2,311,472 + -0.6 2,311,472 + -1.7 2,311,472 + +1.0 2,311,472 + +1.1 2,311,473 - -0.1 2,311,473 - +0.2 2,311,473 - -1.9 2,311,473 - -1.7 2,311,473 - -1.2 2,311,473 - -2.4 2,311,473 - -1.7 2,311,473 - +0.3 2,311,473 - -1.8 2,311,473 - +0.7 2,311,473 - -3.1 2,311,473 - -0.7 2,311,473 - -1.9 2,311,473 - -1.3 2,311,473 - -1.6 2,311,473 - -1.6 2,311,473 - +0.4 2,311,473 - -1.9 2,311,473 - -0.7 2,311,473 - -2.1 2,311,473 - -2.6 2,311,473 - -3.7 2,311,473 - -2.2 2,311,473 - -1.1 2,311,473 - -0.3 2,311,473 - -0.6 2,311,473 - -0.6 2,311,473 - -1.9 2,311,473 - -2.5 2,311,473 - -1.2 2,311,473 - -2.0 2,311,473 - -0.2 2,311,473 - -1.3 2,311,473 - -3.4 2,311,473 - -2.4 2,311,473 - -2.1 2,311,473 - -2.4 2,311,473 - -1.1 2,311,473 - -1.7 2,311,473 - -2.9 2,311,473 - -1.9 2,311,473 - -1.7 2,311,473 - -2.6 2,311,476 + +0.8 2,311,476 + +1.3 2,311,476 + +2.3 2,311,476 + +1.3 2,311,476 + +0.8 2,311,476 + -1.6 2,311,476 + -1.6 2,311,476 + +0.2 2,311,476 + -0.9 2,311,476 + +1.0 2,311,477 - -0.1 2,311,477 - -1.5 2,311,477 - -1.4 2,311,477 - -1.1 2,311,477 - -3.0 2,311,477 - -1.7 2,311,477 - -0.3 2,311,477 - -1.7 2,311,477 - -1.3 2,311,477 - -0.6 2,311,477 - -2.1 2,311,477 - -2.3 2,311,477 - -2.6 2,311,477 - -0.6 2,311,477 - -0.5 2,311,477 - -2.3 2,311,477 - -1.0 2,311,477 - -3.3 2,311,477 - -3.2 2,311,477 - -0.7 2,311,477 - -1.9 2,311,477 - -1.2 2,311,477 - -2.0 2,311,477 - -2.2 2,311,560 + +0.7 2,311,561 - -1.7 2,311,561 - +0.4 2,311,561 - -2.1 2,311,561 - -2.3 2,311,561 - -3.1 2,311,561 - -0.1 2,311,561 - -1.2 2,311,561 - -1.7 2,311,561 - -0.1 2,311,561 - -1.7 2,311,561 - -0.7 2,311,561 - -1.6 2,311,564 + +0.5 2,311,564 + +0.3 2,311,564 + +0.1 2,311,564 + +1.3 2,311,565 - -2.2 2,311,565 - -1.4 2,311,565 - +0.3 2,311,565 - -0.3 2,311,565 - -0.9 2,311,565 - -2.8 2,311,565 - +0.2 2,311,565 - -0.6 2,311,565 - -0.6 2,311,734 - -0.4 2,311,734 - -0.7 2,311,734 - -1.3 2,311,734 - +0.3 2,311,767 + +1.8 2,311,767 + +0.7 2,311,768 - +0.6 2,311,768 - +1.3 2,311,982 - Pf1N1B4_2025 0.12 -2.2
Or see this region's nucleotide sequence