Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2022 and Pf1N1B4_2023 are separated by 173 nucleotides Pf1N1B4_2023 and Pf1N1B4_2024 are separated by 175 nucleotides
Pf1N1B4_2022 - Transcriptional regulator, AraC family, at 2,307,473 to 2,308,471
_2022
Pf1N1B4_2023 - L-asparaginase (EC 3.5.1.1) (from data) , at 2,308,645 to 2,309,649
_2023
Pf1N1B4_2024 - sodium/alanine transporter, at 2,309,825 to 2,311,276
_2024
Position (kb)
2308
2309
2310 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2308.158 kb on - strand at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.214 kb on + strand, within Pf1N1B4_2022 at 2308.215 kb on - strand, within Pf1N1B4_2022 at 2308.215 kb on - strand, within Pf1N1B4_2022 at 2308.237 kb on - strand, within Pf1N1B4_2022 at 2308.286 kb on + strand at 2308.286 kb on + strand, within Pf1N1B4_2022 at 2308.287 kb on - strand, within Pf1N1B4_2022 at 2308.488 kb on + strand at 2308.488 kb on + strand at 2308.488 kb on + strand at 2308.489 kb on - strand at 2308.489 kb on - strand at 2308.492 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.511 kb on + strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.512 kb on - strand at 2308.513 kb on + strand at 2308.513 kb on + strand at 2308.513 kb on + strand at 2308.584 kb on + strand at 2308.585 kb on - strand at 2308.585 kb on - strand at 2308.718 kb on - strand at 2308.805 kb on + strand, within Pf1N1B4_2023 at 2308.805 kb on + strand, within Pf1N1B4_2023 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2309.037 kb on + strand, within Pf1N1B4_2023 at 2309.038 kb on - strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.339 kb on + strand, within Pf1N1B4_2023 at 2309.340 kb on - strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.753 kb on + strand at 2309.754 kb on - strand at 2310.126 kb on - strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.199 kb on - strand at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.208 kb on - strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,307,649 - Pf1N1B4_2022 0.18 -1.3 2,307,649 - Pf1N1B4_2022 0.18 -0.1 2,307,649 - Pf1N1B4_2022 0.18 +2.3 2,307,755 + Pf1N1B4_2022 0.28 -1.1 2,307,755 + Pf1N1B4_2022 0.28 +1.0 2,307,755 + Pf1N1B4_2022 0.28 +1.0 2,307,756 - Pf1N1B4_2022 0.28 -0.2 2,307,756 - Pf1N1B4_2022 0.28 -0.1 2,307,756 - Pf1N1B4_2022 0.28 +0.0 2,307,756 - Pf1N1B4_2022 0.28 -0.2 2,308,158 - -0.7 2,308,196 + Pf1N1B4_2022 0.72 +0.5 2,308,196 + Pf1N1B4_2022 0.72 -0.1 2,308,196 + Pf1N1B4_2022 0.72 -0.4 2,308,196 + Pf1N1B4_2022 0.72 +0.7 2,308,197 - Pf1N1B4_2022 0.72 +0.0 2,308,197 - Pf1N1B4_2022 0.72 -3.3 2,308,197 - Pf1N1B4_2022 0.72 +0.3 2,308,214 + Pf1N1B4_2022 0.74 +2.3 2,308,215 - Pf1N1B4_2022 0.74 +0.2 2,308,215 - Pf1N1B4_2022 0.74 -0.3 2,308,237 - Pf1N1B4_2022 0.76 -0.5 2,308,286 + +1.8 2,308,286 + Pf1N1B4_2022 0.81 +1.5 2,308,287 - Pf1N1B4_2022 0.81 -0.7 2,308,488 + +0.4 2,308,488 + +0.4 2,308,488 + -0.5 2,308,489 - +0.4 2,308,489 - -1.4 2,308,492 + +0.1 2,308,511 + -0.2 2,308,511 + -0.5 2,308,511 + +1.3 2,308,511 + +1.5 2,308,511 + -0.0 2,308,512 - -0.3 2,308,512 - +1.4 2,308,512 - -0.2 2,308,512 - -1.7 2,308,512 - -0.7 2,308,512 - +1.8 2,308,512 - +0.7 2,308,512 - +0.8 2,308,513 + -1.1 2,308,513 + +0.5 2,308,513 + +1.5 2,308,584 + -1.1 2,308,585 - +0.7 2,308,585 - +2.0 2,308,718 - +2.4 2,308,805 + Pf1N1B4_2023 0.16 -0.1 2,308,805 + Pf1N1B4_2023 0.16 +0.3 2,308,931 + Pf1N1B4_2023 0.28 +2.5 2,308,931 + Pf1N1B4_2023 0.28 +1.3 2,309,037 + Pf1N1B4_2023 0.39 +2.0 2,309,038 - Pf1N1B4_2023 0.39 +1.6 2,309,048 + Pf1N1B4_2023 0.40 +0.6 2,309,048 + Pf1N1B4_2023 0.40 +1.5 2,309,048 + Pf1N1B4_2023 0.40 +1.4 2,309,049 - Pf1N1B4_2023 0.40 +2.3 2,309,049 - Pf1N1B4_2023 0.40 +1.8 2,309,049 - Pf1N1B4_2023 0.40 +2.7 2,309,049 - Pf1N1B4_2023 0.40 +1.4 2,309,177 + Pf1N1B4_2023 0.53 +1.0 2,309,177 + Pf1N1B4_2023 0.53 -0.3 2,309,177 + Pf1N1B4_2023 0.53 +1.5 2,309,178 - Pf1N1B4_2023 0.53 +0.2 2,309,178 - Pf1N1B4_2023 0.53 -1.9 2,309,178 - Pf1N1B4_2023 0.53 +1.3 2,309,178 - Pf1N1B4_2023 0.53 +0.8 2,309,178 - Pf1N1B4_2023 0.53 +1.4 2,309,178 - Pf1N1B4_2023 0.53 +0.9 2,309,339 + Pf1N1B4_2023 0.69 +0.7 2,309,340 - Pf1N1B4_2023 0.69 +0.4 2,309,522 + Pf1N1B4_2023 0.87 +0.5 2,309,522 + Pf1N1B4_2023 0.87 -1.0 2,309,522 + Pf1N1B4_2023 0.87 +0.3 2,309,523 - Pf1N1B4_2023 0.87 +1.4 2,309,523 - Pf1N1B4_2023 0.87 +0.6 2,309,604 - +1.3 2,309,604 - +0.3 2,309,604 - +0.6 2,309,604 - +0.5 2,309,604 - +0.4 2,309,604 - +1.1 2,309,753 + -1.7 2,309,754 - +1.3 2,310,126 - Pf1N1B4_2024 0.21 +1.5 2,310,198 + Pf1N1B4_2024 0.26 +0.5 2,310,198 + Pf1N1B4_2024 0.26 +1.0 2,310,198 + Pf1N1B4_2024 0.26 -0.3 2,310,199 - +2.8 2,310,199 - Pf1N1B4_2024 0.26 -0.3 2,310,199 - Pf1N1B4_2024 0.26 +1.6 2,310,201 + Pf1N1B4_2024 0.26 +2.1 2,310,201 + Pf1N1B4_2024 0.26 +2.7 2,310,201 + Pf1N1B4_2024 0.26 -1.6 2,310,201 + Pf1N1B4_2024 0.26 +1.1 2,310,201 + Pf1N1B4_2024 0.26 +0.0 2,310,201 + Pf1N1B4_2024 0.26 +0.9 2,310,202 - Pf1N1B4_2024 0.26 -0.3 2,310,202 - Pf1N1B4_2024 0.26 +1.3 2,310,202 - Pf1N1B4_2024 0.26 -0.2 2,310,202 - Pf1N1B4_2024 0.26 +1.1 2,310,202 - Pf1N1B4_2024 0.26 +0.3 2,310,202 - Pf1N1B4_2024 0.26 +0.5 2,310,202 - Pf1N1B4_2024 0.26 +1.5 2,310,202 - Pf1N1B4_2024 0.26 +0.6 2,310,202 - Pf1N1B4_2024 0.26 +0.7 2,310,207 + Pf1N1B4_2024 0.26 +0.5 2,310,207 + Pf1N1B4_2024 0.26 +1.8 2,310,208 - Pf1N1B4_2024 0.26 +2.1 2,310,333 + Pf1N1B4_2024 0.35 -0.9 2,310,333 + Pf1N1B4_2024 0.35 +1.6 2,310,624 + Pf1N1B4_2024 0.55 -1.4 2,310,624 + Pf1N1B4_2024 0.55 -1.9 2,310,624 + Pf1N1B4_2024 0.55 +0.7 2,310,624 + Pf1N1B4_2024 0.55 +0.4 2,310,624 + Pf1N1B4_2024 0.55 +0.1 2,310,625 - Pf1N1B4_2024 0.55 +0.5 2,310,625 - Pf1N1B4_2024 0.55 -1.6 2,310,625 - Pf1N1B4_2024 0.55 +0.7 2,310,625 - Pf1N1B4_2024 0.55 -0.3 2,310,625 - Pf1N1B4_2024 0.55 -0.6 2,310,625 - Pf1N1B4_2024 0.55 +0.5 2,310,625 - Pf1N1B4_2024 0.55 +1.0 2,310,642 + Pf1N1B4_2024 0.56 +1.0 2,310,642 + Pf1N1B4_2024 0.56 -0.3 2,310,642 + Pf1N1B4_2024 0.56 +3.3 2,310,642 + Pf1N1B4_2024 0.56 -0.7 2,310,642 + Pf1N1B4_2024 0.56 +1.6 2,310,642 + Pf1N1B4_2024 0.56 +0.7 2,310,643 - Pf1N1B4_2024 0.56 +1.3 2,310,643 - Pf1N1B4_2024 0.56 +0.0 2,310,643 - Pf1N1B4_2024 0.56 +0.5 2,310,643 - Pf1N1B4_2024 0.56 +0.3
Or see this region's nucleotide sequence