Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_199

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_196 and Pf1N1B4_197 overlap by 4 nucleotidesPf1N1B4_197 and Pf1N1B4_198 overlap by 4 nucleotidesPf1N1B4_198 and Pf1N1B4_199 overlap by 4 nucleotidesPf1N1B4_199 and Pf1N1B4_200 are separated by 35 nucleotidesPf1N1B4_200 and Pf1N1B4_201 overlap by 4 nucleotides Pf1N1B4_196 - FIG137594: Putative iron-regulated membrane protein, at 244,571 to 245,776 _196 Pf1N1B4_197 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 245,773 to 246,315 _197 Pf1N1B4_198 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 246,312 to 246,632 _198 Pf1N1B4_199 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 246,629 to 247,204 _199 Pf1N1B4_200 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 247,240 to 248,169 _200 Pf1N1B4_201 - ABC transporter in pyoverdin gene cluster, ATP-binding component, at 248,166 to 249,017 _201 Position (kb) 246 247 248Strain fitness (log2 ratio) -2 -1 0 1 2at 245.887 kb on + strand, within Pf1N1B4_197at 245.888 kb on - strand, within Pf1N1B4_197at 245.888 kb on - strand, within Pf1N1B4_197at 246.025 kb on + strand, within Pf1N1B4_197at 246.025 kb on + strandat 246.337 kb on + strandat 246.338 kb on - strandat 246.338 kb on - strandat 246.338 kb on - strandat 246.341 kb on - strandat 246.564 kb on + strand, within Pf1N1B4_198at 246.564 kb on + strand, within Pf1N1B4_198at 246.564 kb on + strandat 246.564 kb on + strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.565 kb on - strand, within Pf1N1B4_198at 246.634 kb on + strandat 246.634 kb on + strandat 246.635 kb on - strandat 246.635 kb on - strandat 246.635 kb on - strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.686 kb on + strandat 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.687 kb on - strand, within Pf1N1B4_199at 246.824 kb on - strand, within Pf1N1B4_199at 246.824 kb on - strand, within Pf1N1B4_199at 246.824 kb on - strand, within Pf1N1B4_199at 246.824 kb on - strandat 246.824 kb on - strand, within Pf1N1B4_199at 246.824 kb on - strand, within Pf1N1B4_199at 246.828 kb on - strand, within Pf1N1B4_199at 246.828 kb on - strand, within Pf1N1B4_199at 246.996 kb on - strand, within Pf1N1B4_199at 246.996 kb on - strand, within Pf1N1B4_199at 247.245 kb on + strandat 247.246 kb on - strandat 247.246 kb on - strandat 247.246 kb on - strandat 247.246 kb on - strandat 247.369 kb on + strandat 247.369 kb on + strand, within Pf1N1B4_200at 247.370 kb on - strand, within Pf1N1B4_200at 247.372 kb on + strand, within Pf1N1B4_200at 247.372 kb on + strand, within Pf1N1B4_200at 247.372 kb on + strand, within Pf1N1B4_200at 247.372 kb on + strand, within Pf1N1B4_200at 247.373 kb on - strand, within Pf1N1B4_200at 247.373 kb on - strand, within Pf1N1B4_200at 247.373 kb on - strand, within Pf1N1B4_200at 247.373 kb on - strand, within Pf1N1B4_200at 247.373 kb on - strand, within Pf1N1B4_200at 247.378 kb on + strand, within Pf1N1B4_200at 247.378 kb on + strand, within Pf1N1B4_200at 247.378 kb on + strand, within Pf1N1B4_200at 247.378 kb on + strand, within Pf1N1B4_200at 247.379 kb on - strand, within Pf1N1B4_200at 247.379 kb on - strand, within Pf1N1B4_200at 247.379 kb on - strand, within Pf1N1B4_200at 247.379 kb on - strand, within Pf1N1B4_200at 247.451 kb on - strand, within Pf1N1B4_200at 247.451 kb on - strand, within Pf1N1B4_200at 247.502 kb on + strand, within Pf1N1B4_200at 247.502 kb on + strand, within Pf1N1B4_200at 247.506 kb on + strand, within Pf1N1B4_200at 247.744 kb on + strand, within Pf1N1B4_200at 247.867 kb on + strand, within Pf1N1B4_200at 247.868 kb on - strand, within Pf1N1B4_200at 248.020 kb on + strand, within Pf1N1B4_200at 248.020 kb on + strand, within Pf1N1B4_200at 248.021 kb on - strand, within Pf1N1B4_200

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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245,887 + Pf1N1B4_197 0.21 -2.2
245,888 - Pf1N1B4_197 0.21 +1.1
245,888 - Pf1N1B4_197 0.21 +1.8
246,025 + Pf1N1B4_197 0.46 +0.5
246,025 + -1.5
246,337 + +0.4
246,338 - -1.8
246,338 - -0.2
246,338 - +0.5
246,341 - +0.4
246,564 + Pf1N1B4_198 0.79 +0.4
246,564 + Pf1N1B4_198 0.79 -0.4
246,564 + +1.2
246,564 + Pf1N1B4_198 0.79 -0.2
246,565 - Pf1N1B4_198 0.79 +0.6
246,565 - Pf1N1B4_198 0.79 +0.5
246,565 - Pf1N1B4_198 0.79 -1.5
246,565 - Pf1N1B4_198 0.79 +1.1
246,634 + -0.8
246,634 + +1.3
246,635 - -0.2
246,635 - +0.8
246,635 - -0.4
246,686 + +0.8
246,686 + +1.3
246,686 + -0.3
246,686 + +1.0
246,686 + -1.2
246,686 + -0.8
246,686 + +0.8
246,687 - Pf1N1B4_199 0.10 -1.2
246,687 - Pf1N1B4_199 0.10 -1.0
246,687 - Pf1N1B4_199 0.10 -0.6
246,687 - Pf1N1B4_199 0.10 +0.8
246,687 - Pf1N1B4_199 0.10 -0.2
246,687 - Pf1N1B4_199 0.10 +1.0
246,687 - Pf1N1B4_199 0.10 -0.7
246,824 - Pf1N1B4_199 0.34 -2.0
246,824 - Pf1N1B4_199 0.34 +0.8
246,824 - Pf1N1B4_199 0.34 +1.1
246,824 - +1.8
246,824 - Pf1N1B4_199 0.34 -1.5
246,824 - Pf1N1B4_199 0.34 -1.1
246,828 - Pf1N1B4_199 0.35 -0.8
246,828 - Pf1N1B4_199 0.35 -1.3
246,996 - Pf1N1B4_199 0.64 +0.2
246,996 - Pf1N1B4_199 0.64 -1.8
247,245 + +0.2
247,246 - -1.2
247,246 - +0.4
247,246 - +1.0
247,246 - -1.2
247,369 + +0.8
247,369 + Pf1N1B4_200 0.14 -0.8
247,370 - Pf1N1B4_200 0.14 -0.8
247,372 + Pf1N1B4_200 0.14 +0.6
247,372 + Pf1N1B4_200 0.14 +0.1
247,372 + Pf1N1B4_200 0.14 +0.8
247,372 + Pf1N1B4_200 0.14 -0.8
247,373 - Pf1N1B4_200 0.14 +0.6
247,373 - Pf1N1B4_200 0.14 +0.8
247,373 - Pf1N1B4_200 0.14 +1.5
247,373 - Pf1N1B4_200 0.14 -1.2
247,373 - Pf1N1B4_200 0.14 +0.4
247,378 + Pf1N1B4_200 0.15 +2.4
247,378 + Pf1N1B4_200 0.15 -0.9
247,378 + Pf1N1B4_200 0.15 -1.2
247,378 + Pf1N1B4_200 0.15 +0.6
247,379 - Pf1N1B4_200 0.15 -0.5
247,379 - Pf1N1B4_200 0.15 -0.3
247,379 - Pf1N1B4_200 0.15 -0.8
247,379 - Pf1N1B4_200 0.15 +0.8
247,451 - Pf1N1B4_200 0.23 -1.4
247,451 - Pf1N1B4_200 0.23 -1.2
247,502 + Pf1N1B4_200 0.28 +0.3
247,502 + Pf1N1B4_200 0.28 +0.1
247,506 + Pf1N1B4_200 0.29 +0.6
247,744 + Pf1N1B4_200 0.54 +0.2
247,867 + Pf1N1B4_200 0.67 -1.3
247,868 - Pf1N1B4_200 0.68 +0.5
248,020 + Pf1N1B4_200 0.84 +0.2
248,020 + Pf1N1B4_200 0.84 -2.2
248,021 - Pf1N1B4_200 0.84 -2.0

Or see this region's nucleotide sequence