Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1962 and Pf1N1B4_1963 are separated by 14 nucleotides Pf1N1B4_1963 and Pf1N1B4_1964 are separated by 0 nucleotides Pf1N1B4_1964 and Pf1N1B4_1965 are separated by 3 nucleotides Pf1N1B4_1965 and Pf1N1B4_1966 overlap by 4 nucleotides
Pf1N1B4_1962 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 2,242,531 to 2,243,769
_1962
Pf1N1B4_1963 - O-antigen export system permease protein RfbD, at 2,243,784 to 2,244,581
_1963
Pf1N1B4_1964 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 2,244,582 to 2,246,024
_1964
Pf1N1B4_1965 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 2,246,028 to 2,246,999
_1965
Pf1N1B4_1966 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,246,996 to 2,247,754
_1966
Position (kb)
2244
2245
2246
2247 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2243.589 kb on + strand, within Pf1N1B4_1962 at 2243.590 kb on - strand, within Pf1N1B4_1962 at 2243.590 kb on - strand, within Pf1N1B4_1962 at 2243.590 kb on - strand, within Pf1N1B4_1962 at 2243.653 kb on + strand at 2243.654 kb on - strand at 2243.654 kb on - strand at 2243.654 kb on - strand at 2243.738 kb on + strand at 2243.747 kb on + strand at 2243.747 kb on + strand at 2243.748 kb on - strand at 2243.748 kb on - strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.920 kb on + strand, within Pf1N1B4_1963 at 2243.921 kb on - strand, within Pf1N1B4_1963 at 2243.921 kb on - strand at 2244.364 kb on + strand, within Pf1N1B4_1963 at 2244.364 kb on + strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.373 kb on + strand, within Pf1N1B4_1963 at 2244.373 kb on + strand, within Pf1N1B4_1963 at 2244.374 kb on - strand, within Pf1N1B4_1963 at 2244.374 kb on - strand, within Pf1N1B4_1963 at 2244.374 kb on - strand, within Pf1N1B4_1963 at 2244.436 kb on + strand, within Pf1N1B4_1963 at 2244.436 kb on + strand at 2244.436 kb on + strand, within Pf1N1B4_1963 at 2244.437 kb on - strand, within Pf1N1B4_1963 at 2244.437 kb on - strand, within Pf1N1B4_1963 at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.638 kb on - strand at 2244.857 kb on + strand, within Pf1N1B4_1964 at 2244.858 kb on - strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.938 kb on - strand, within Pf1N1B4_1964 at 2245.047 kb on - strand, within Pf1N1B4_1964 at 2245.250 kb on - strand, within Pf1N1B4_1964 at 2245.460 kb on - strand, within Pf1N1B4_1964 at 2245.460 kb on - strand, within Pf1N1B4_1964 at 2245.460 kb on - strand, within Pf1N1B4_1964 at 2245.837 kb on + strand, within Pf1N1B4_1964 at 2245.837 kb on + strand, within Pf1N1B4_1964 at 2245.837 kb on + strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.840 kb on + strand, within Pf1N1B4_1964 at 2245.840 kb on + strand, within Pf1N1B4_1964 at 2245.910 kb on + strand at 2245.911 kb on - strand at 2245.911 kb on - strand at 2245.911 kb on - strand at 2245.911 kb on - strand at 2246.029 kb on + strand at 2246.030 kb on - strand at 2246.030 kb on - strand at 2246.151 kb on + strand, within Pf1N1B4_1965 at 2246.152 kb on - strand, within Pf1N1B4_1965 at 2246.191 kb on + strand, within Pf1N1B4_1965 at 2246.191 kb on + strand, within Pf1N1B4_1965 at 2246.192 kb on - strand, within Pf1N1B4_1965 at 2246.201 kb on - strand, within Pf1N1B4_1965 at 2246.201 kb on - strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.323 kb on + strand, within Pf1N1B4_1965 at 2246.323 kb on + strand, within Pf1N1B4_1965 at 2246.324 kb on - strand, within Pf1N1B4_1965 at 2246.533 kb on + strand, within Pf1N1B4_1965 at 2246.635 kb on + strand, within Pf1N1B4_1965 at 2246.635 kb on + strand, within Pf1N1B4_1965 at 2246.635 kb on + strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.764 kb on + strand, within Pf1N1B4_1965 at 2246.764 kb on + strand, within Pf1N1B4_1965 at 2246.765 kb on - strand, within Pf1N1B4_1965 at 2246.765 kb on - strand, within Pf1N1B4_1965 at 2246.837 kb on - strand, within Pf1N1B4_1965 at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.920 kb on + strand at 2246.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,243,589 + Pf1N1B4_1962 0.85 +0.2 2,243,590 - Pf1N1B4_1962 0.85 -0.1 2,243,590 - Pf1N1B4_1962 0.85 -0.9 2,243,590 - Pf1N1B4_1962 0.85 +0.4 2,243,653 + +0.2 2,243,654 - -1.3 2,243,654 - +0.1 2,243,654 - -0.9 2,243,738 + -0.0 2,243,747 + -0.8 2,243,747 + +1.6 2,243,748 - +0.1 2,243,748 - -1.3 2,243,773 + +0.1 2,243,773 + -0.9 2,243,773 + -0.0 2,243,773 + +0.2 2,243,773 + +0.7 2,243,773 + -1.6 2,243,773 + +0.3 2,243,773 + +1.1 2,243,773 + -1.1 2,243,773 + +0.9 2,243,774 - -1.6 2,243,774 - -1.3 2,243,774 - -0.3 2,243,774 - +2.8 2,243,774 - +1.2 2,243,774 - -0.8 2,243,774 - +0.5 2,243,774 - -0.3 2,243,774 - -2.3 2,243,920 + Pf1N1B4_1963 0.17 -1.1 2,243,921 - Pf1N1B4_1963 0.17 +0.2 2,243,921 - -0.3 2,244,364 + Pf1N1B4_1963 0.73 -2.2 2,244,364 + Pf1N1B4_1963 0.73 -1.0 2,244,365 - Pf1N1B4_1963 0.73 -1.6 2,244,365 - Pf1N1B4_1963 0.73 -1.3 2,244,365 - Pf1N1B4_1963 0.73 -0.8 2,244,365 - Pf1N1B4_1963 0.73 +1.2 2,244,365 - Pf1N1B4_1963 0.73 +0.8 2,244,373 + Pf1N1B4_1963 0.74 -0.8 2,244,373 + Pf1N1B4_1963 0.74 +0.7 2,244,374 - Pf1N1B4_1963 0.74 -0.3 2,244,374 - Pf1N1B4_1963 0.74 +1.4 2,244,374 - Pf1N1B4_1963 0.74 +1.1 2,244,436 + Pf1N1B4_1963 0.82 -2.7 2,244,436 + +1.2 2,244,436 + Pf1N1B4_1963 0.82 +0.2 2,244,437 - Pf1N1B4_1963 0.82 +0.7 2,244,437 - Pf1N1B4_1963 0.82 +0.4 2,244,637 + +0.8 2,244,637 + +1.8 2,244,637 + -0.8 2,244,637 + -0.8 2,244,637 + -1.6 2,244,638 - -0.9 2,244,857 + Pf1N1B4_1964 0.19 +0.1 2,244,858 - Pf1N1B4_1964 0.19 +0.8 2,244,898 + Pf1N1B4_1964 0.22 -1.3 2,244,898 + Pf1N1B4_1964 0.22 +0.6 2,244,898 + Pf1N1B4_1964 0.22 +1.2 2,244,898 + Pf1N1B4_1964 0.22 -0.3 2,244,898 + Pf1N1B4_1964 0.22 -0.8 2,244,898 + -0.6 2,244,898 + Pf1N1B4_1964 0.22 +0.7 2,244,899 - Pf1N1B4_1964 0.22 +1.7 2,244,899 - Pf1N1B4_1964 0.22 -0.0 2,244,899 - Pf1N1B4_1964 0.22 +0.1 2,244,899 - Pf1N1B4_1964 0.22 -1.1 2,244,899 - Pf1N1B4_1964 0.22 +0.6 2,244,899 - Pf1N1B4_1964 0.22 -0.7 2,244,899 - Pf1N1B4_1964 0.22 +1.8 2,244,938 - Pf1N1B4_1964 0.25 +1.2 2,245,047 - Pf1N1B4_1964 0.32 +0.9 2,245,250 - Pf1N1B4_1964 0.46 -0.6 2,245,460 - Pf1N1B4_1964 0.61 +0.5 2,245,460 - Pf1N1B4_1964 0.61 +1.2 2,245,460 - Pf1N1B4_1964 0.61 -0.5 2,245,837 + Pf1N1B4_1964 0.87 +1.6 2,245,837 + Pf1N1B4_1964 0.87 +0.2 2,245,837 + Pf1N1B4_1964 0.87 +1.2 2,245,838 - Pf1N1B4_1964 0.87 -1.8 2,245,838 - Pf1N1B4_1964 0.87 +1.0 2,245,838 - Pf1N1B4_1964 0.87 +0.8 2,245,838 - Pf1N1B4_1964 0.87 -0.3 2,245,838 - Pf1N1B4_1964 0.87 -0.4 2,245,838 - Pf1N1B4_1964 0.87 +0.1 2,245,838 - +0.7 2,245,838 - Pf1N1B4_1964 0.87 +0.5 2,245,840 + Pf1N1B4_1964 0.87 +0.2 2,245,840 + Pf1N1B4_1964 0.87 +0.9 2,245,910 + -2.6 2,245,911 - -0.7 2,245,911 - +0.7 2,245,911 - +0.1 2,245,911 - -2.7 2,246,029 + -0.5 2,246,030 - +0.8 2,246,030 - +1.2 2,246,151 + Pf1N1B4_1965 0.13 +0.7 2,246,152 - Pf1N1B4_1965 0.13 -0.3 2,246,191 + Pf1N1B4_1965 0.17 +1.0 2,246,191 + Pf1N1B4_1965 0.17 +1.1 2,246,192 - Pf1N1B4_1965 0.17 -0.8 2,246,201 - Pf1N1B4_1965 0.18 +1.2 2,246,201 - Pf1N1B4_1965 0.18 +0.1 2,246,275 + Pf1N1B4_1965 0.25 -0.6 2,246,275 + Pf1N1B4_1965 0.25 -1.3 2,246,275 + Pf1N1B4_1965 0.25 +0.9 2,246,275 + Pf1N1B4_1965 0.25 +0.1 2,246,275 + Pf1N1B4_1965 0.25 +0.5 2,246,275 + Pf1N1B4_1965 0.25 +0.7 2,246,275 + Pf1N1B4_1965 0.25 -0.3 2,246,275 + Pf1N1B4_1965 0.25 +0.0 2,246,275 + Pf1N1B4_1965 0.25 -0.2 2,246,275 + Pf1N1B4_1965 0.25 -2.2 2,246,276 - Pf1N1B4_1965 0.26 +1.1 2,246,276 - Pf1N1B4_1965 0.26 +0.2 2,246,276 - Pf1N1B4_1965 0.26 -0.2 2,246,276 - Pf1N1B4_1965 0.26 +0.7 2,246,276 - Pf1N1B4_1965 0.26 -2.7 2,246,276 - Pf1N1B4_1965 0.26 -1.1 2,246,323 + Pf1N1B4_1965 0.30 -0.3 2,246,323 + Pf1N1B4_1965 0.30 -0.6 2,246,324 - Pf1N1B4_1965 0.30 +0.6 2,246,533 + Pf1N1B4_1965 0.52 -0.8 2,246,635 + Pf1N1B4_1965 0.62 -1.1 2,246,635 + Pf1N1B4_1965 0.62 +0.1 2,246,635 + Pf1N1B4_1965 0.62 -1.6 2,246,636 - Pf1N1B4_1965 0.63 +0.2 2,246,636 - Pf1N1B4_1965 0.63 +1.2 2,246,636 - Pf1N1B4_1965 0.63 +0.3 2,246,636 - Pf1N1B4_1965 0.63 +0.9 2,246,636 - Pf1N1B4_1965 0.63 -1.3 2,246,764 + Pf1N1B4_1965 0.76 +0.3 2,246,764 + Pf1N1B4_1965 0.76 +0.7 2,246,765 - Pf1N1B4_1965 0.76 +1.2 2,246,765 - Pf1N1B4_1965 0.76 +1.2 2,246,837 - Pf1N1B4_1965 0.83 +2.2 2,246,918 + -0.6 2,246,918 + -0.8 2,246,918 + +1.2 2,246,918 + +0.4 2,246,918 + -0.3 2,246,919 - +0.2 2,246,919 - -0.5 2,246,919 - +1.2 2,246,919 - -1.6 2,246,919 - +1.2 2,246,919 - -0.1 2,246,919 - -0.2 2,246,919 - +1.6 2,246,919 - +1.5 2,246,920 + +0.7 2,246,950 + +0.7
Or see this region's nucleotide sequence