Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1837 and Pf1N1B4_1838 are separated by 171 nucleotides Pf1N1B4_1838 and Pf1N1B4_1839 are separated by 6 nucleotides Pf1N1B4_1839 and Pf1N1B4_1840 are separated by 40 nucleotides Pf1N1B4_1840 and Pf1N1B4_1841 are separated by 121 nucleotides
Pf1N1B4_1837 - Osmotically inducible protein C, at 2,111,899 to 2,112,330
_1837
Pf1N1B4_1838 - FIG00955509: hypothetical protein, at 2,112,502 to 2,112,735
_1838
Pf1N1B4_1839 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,112,742 to 2,113,161
_1839
Pf1N1B4_1840 - lipoprotein, putative, at 2,113,202 to 2,113,618
_1840
Pf1N1B4_1841 - PUTATIVE ZINC PROTEASE PROTEIN, at 2,113,740 to 2,114,768
_1841
Position (kb)
2112
2113
2114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2111.746 kb on + strand at 2111.747 kb on - strand at 2111.747 kb on - strand at 2111.748 kb on + strand at 2111.780 kb on + strand at 2111.828 kb on + strand at 2111.829 kb on - strand at 2111.905 kb on - strand at 2111.905 kb on - strand at 2112.118 kb on - strand, within Pf1N1B4_1837 at 2112.164 kb on + strand, within Pf1N1B4_1837 at 2112.191 kb on + strand, within Pf1N1B4_1837 at 2112.331 kb on + strand at 2112.343 kb on - strand at 2112.343 kb on - strand at 2112.343 kb on - strand at 2112.347 kb on + strand at 2112.348 kb on - strand at 2112.348 kb on - strand at 2112.446 kb on - strand at 2112.526 kb on - strand, within Pf1N1B4_1838 at 2112.628 kb on + strand, within Pf1N1B4_1838 at 2112.628 kb on + strand, within Pf1N1B4_1838 at 2112.628 kb on + strand, within Pf1N1B4_1838 at 2112.629 kb on - strand, within Pf1N1B4_1838 at 2112.715 kb on + strand at 2112.715 kb on + strand at 2112.716 kb on - strand at 2112.716 kb on - strand at 2112.716 kb on - strand at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.797 kb on + strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.798 kb on - strand, within Pf1N1B4_1839 at 2112.823 kb on + strand, within Pf1N1B4_1839 at 2112.823 kb on + strand, within Pf1N1B4_1839 at 2112.824 kb on - strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.825 kb on + strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.826 kb on - strand, within Pf1N1B4_1839 at 2112.845 kb on + strand, within Pf1N1B4_1839 at 2112.846 kb on - strand, within Pf1N1B4_1839 at 2112.872 kb on + strand, within Pf1N1B4_1839 at 2112.872 kb on + strand, within Pf1N1B4_1839 at 2112.872 kb on + strand, within Pf1N1B4_1839 at 2112.873 kb on - strand, within Pf1N1B4_1839 at 2112.873 kb on - strand, within Pf1N1B4_1839 at 2112.873 kb on - strand, within Pf1N1B4_1839 at 2112.888 kb on - strand, within Pf1N1B4_1839 at 2112.888 kb on - strand, within Pf1N1B4_1839 at 2112.955 kb on + strand, within Pf1N1B4_1839 at 2112.956 kb on - strand, within Pf1N1B4_1839 at 2112.956 kb on - strand, within Pf1N1B4_1839 at 2112.956 kb on - strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.961 kb on + strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.962 kb on - strand, within Pf1N1B4_1839 at 2112.992 kb on + strand, within Pf1N1B4_1839 at 2112.992 kb on + strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2112.993 kb on - strand, within Pf1N1B4_1839 at 2113.183 kb on + strand at 2113.207 kb on + strand at 2113.207 kb on + strand at 2113.207 kb on + strand at 2113.207 kb on + strand at 2113.208 kb on + strand at 2113.208 kb on - strand at 2113.208 kb on - strand at 2113.260 kb on + strand, within Pf1N1B4_1840 at 2113.260 kb on + strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.261 kb on - strand, within Pf1N1B4_1840 at 2113.288 kb on + strand, within Pf1N1B4_1840 at 2113.290 kb on + strand, within Pf1N1B4_1840 at 2113.290 kb on + strand, within Pf1N1B4_1840 at 2113.291 kb on - strand, within Pf1N1B4_1840 at 2113.291 kb on - strand, within Pf1N1B4_1840 at 2113.413 kb on + strand, within Pf1N1B4_1840 at 2113.414 kb on - strand, within Pf1N1B4_1840 at 2113.414 kb on - strand, within Pf1N1B4_1840 at 2113.414 kb on - strand, within Pf1N1B4_1840 at 2113.414 kb on - strand, within Pf1N1B4_1840 at 2113.445 kb on + strand, within Pf1N1B4_1840 at 2113.445 kb on + strand, within Pf1N1B4_1840 at 2113.446 kb on - strand, within Pf1N1B4_1840 at 2113.446 kb on - strand, within Pf1N1B4_1840 at 2113.455 kb on + strand, within Pf1N1B4_1840 at 2113.455 kb on + strand, within Pf1N1B4_1840 at 2113.456 kb on - strand, within Pf1N1B4_1840 at 2113.490 kb on + strand, within Pf1N1B4_1840 at 2113.491 kb on - strand, within Pf1N1B4_1840 at 2113.491 kb on - strand, within Pf1N1B4_1840 at 2113.558 kb on + strand, within Pf1N1B4_1840 at 2113.558 kb on + strand, within Pf1N1B4_1840 at 2113.559 kb on - strand, within Pf1N1B4_1840 at 2113.559 kb on - strand, within Pf1N1B4_1840 at 2113.559 kb on - strand, within Pf1N1B4_1840 at 2113.562 kb on + strand, within Pf1N1B4_1840 at 2113.562 kb on + strand, within Pf1N1B4_1840 at 2113.562 kb on + strand, within Pf1N1B4_1840 at 2113.563 kb on - strand, within Pf1N1B4_1840 at 2113.563 kb on - strand, within Pf1N1B4_1840 at 2113.563 kb on - strand, within Pf1N1B4_1840 at 2113.619 kb on - strand at 2113.642 kb on - strand at 2113.705 kb on + strand at 2113.705 kb on + strand at 2113.706 kb on - strand at 2113.706 kb on - strand at 2113.721 kb on - strand at 2113.754 kb on - strand at 2113.779 kb on + strand at 2113.779 kb on + strand at 2113.779 kb on + strand at 2113.779 kb on + strand at 2113.779 kb on + strand at 2113.779 kb on + strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.780 kb on - strand at 2113.782 kb on + strand at 2113.782 kb on + strand at 2113.782 kb on + strand at 2113.959 kb on + strand, within Pf1N1B4_1841 at 2113.959 kb on + strand, within Pf1N1B4_1841 at 2113.984 kb on + strand, within Pf1N1B4_1841 at 2113.984 kb on + strand at 2113.985 kb on - strand, within Pf1N1B4_1841 at 2113.985 kb on - strand, within Pf1N1B4_1841 at 2113.985 kb on - strand, within Pf1N1B4_1841 at 2113.985 kb on - strand, within Pf1N1B4_1841 at 2114.076 kb on + strand, within Pf1N1B4_1841 at 2114.076 kb on + strand, within Pf1N1B4_1841 at 2114.076 kb on + strand, within Pf1N1B4_1841 at 2114.076 kb on + strand, within Pf1N1B4_1841 at 2114.076 kb on + strand, within Pf1N1B4_1841 at 2114.077 kb on - strand, within Pf1N1B4_1841 at 2114.077 kb on - strand, within Pf1N1B4_1841 at 2114.077 kb on - strand, within Pf1N1B4_1841 at 2114.077 kb on - strand, within Pf1N1B4_1841 at 2114.077 kb on - strand, within Pf1N1B4_1841 at 2114.126 kb on + strand, within Pf1N1B4_1841 at 2114.126 kb on + strand, within Pf1N1B4_1841 at 2114.126 kb on + strand, within Pf1N1B4_1841 at 2114.127 kb on - strand, within Pf1N1B4_1841 at 2114.127 kb on - strand, within Pf1N1B4_1841 at 2114.127 kb on - strand, within Pf1N1B4_1841 at 2114.127 kb on - strand, within Pf1N1B4_1841 at 2114.157 kb on + strand, within Pf1N1B4_1841 at 2114.158 kb on - strand, within Pf1N1B4_1841 at 2114.158 kb on - strand, within Pf1N1B4_1841
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,111,746 + +1.2 2,111,747 - -1.1 2,111,747 - +0.5 2,111,748 + -0.8 2,111,780 + +1.2 2,111,828 + +0.6 2,111,829 - -0.9 2,111,905 - -0.2 2,111,905 - +0.9 2,112,118 - Pf1N1B4_1837 0.51 -0.6 2,112,164 + Pf1N1B4_1837 0.61 +0.7 2,112,191 + Pf1N1B4_1837 0.68 -0.1 2,112,331 + -1.8 2,112,343 - +1.5 2,112,343 - -0.2 2,112,343 - +0.8 2,112,347 + -1.4 2,112,348 - +1.2 2,112,348 - -1.8 2,112,446 - -2.1 2,112,526 - Pf1N1B4_1838 0.10 -0.1 2,112,628 + Pf1N1B4_1838 0.54 +1.6 2,112,628 + Pf1N1B4_1838 0.54 +0.5 2,112,628 + Pf1N1B4_1838 0.54 +0.4 2,112,629 - Pf1N1B4_1838 0.54 +1.2 2,112,715 + +1.6 2,112,715 + +0.6 2,112,716 - -2.4 2,112,716 - +0.5 2,112,716 - +0.2 2,112,797 + Pf1N1B4_1839 0.13 -1.2 2,112,797 + Pf1N1B4_1839 0.13 -0.1 2,112,797 + Pf1N1B4_1839 0.13 -1.3 2,112,797 + Pf1N1B4_1839 0.13 +0.8 2,112,798 - Pf1N1B4_1839 0.13 +1.3 2,112,798 - Pf1N1B4_1839 0.13 -1.1 2,112,798 - Pf1N1B4_1839 0.13 -1.2 2,112,798 - Pf1N1B4_1839 0.13 +1.2 2,112,823 + Pf1N1B4_1839 0.19 +1.2 2,112,823 + Pf1N1B4_1839 0.19 +0.8 2,112,824 - Pf1N1B4_1839 0.20 +1.9 2,112,825 + Pf1N1B4_1839 0.20 +1.2 2,112,825 + Pf1N1B4_1839 0.20 +0.4 2,112,825 + Pf1N1B4_1839 0.20 -1.1 2,112,825 + Pf1N1B4_1839 0.20 -0.3 2,112,825 + Pf1N1B4_1839 0.20 +1.4 2,112,825 + Pf1N1B4_1839 0.20 +1.8 2,112,825 + Pf1N1B4_1839 0.20 +0.0 2,112,825 + Pf1N1B4_1839 0.20 +1.2 2,112,826 - Pf1N1B4_1839 0.20 -0.1 2,112,826 - Pf1N1B4_1839 0.20 -0.8 2,112,826 - Pf1N1B4_1839 0.20 +0.2 2,112,826 - Pf1N1B4_1839 0.20 +0.4 2,112,826 - Pf1N1B4_1839 0.20 -0.8 2,112,845 + Pf1N1B4_1839 0.25 -2.1 2,112,846 - Pf1N1B4_1839 0.25 +0.0 2,112,872 + Pf1N1B4_1839 0.31 +0.2 2,112,872 + Pf1N1B4_1839 0.31 -2.8 2,112,872 + Pf1N1B4_1839 0.31 +0.2 2,112,873 - Pf1N1B4_1839 0.31 -0.6 2,112,873 - Pf1N1B4_1839 0.31 -0.1 2,112,873 - Pf1N1B4_1839 0.31 -0.2 2,112,888 - Pf1N1B4_1839 0.35 -0.3 2,112,888 - Pf1N1B4_1839 0.35 -0.3 2,112,955 + Pf1N1B4_1839 0.51 +1.2 2,112,956 - Pf1N1B4_1839 0.51 -0.8 2,112,956 - Pf1N1B4_1839 0.51 -0.8 2,112,956 - Pf1N1B4_1839 0.51 +2.0 2,112,961 + Pf1N1B4_1839 0.52 +2.2 2,112,961 + Pf1N1B4_1839 0.52 +0.7 2,112,961 + Pf1N1B4_1839 0.52 -1.8 2,112,961 + Pf1N1B4_1839 0.52 +0.2 2,112,961 + Pf1N1B4_1839 0.52 -0.3 2,112,961 + Pf1N1B4_1839 0.52 +0.2 2,112,962 - Pf1N1B4_1839 0.52 -1.1 2,112,962 - Pf1N1B4_1839 0.52 +0.5 2,112,962 - Pf1N1B4_1839 0.52 -1.3 2,112,962 - Pf1N1B4_1839 0.52 +0.5 2,112,962 - Pf1N1B4_1839 0.52 +0.9 2,112,962 - Pf1N1B4_1839 0.52 +1.5 2,112,992 + Pf1N1B4_1839 0.60 -0.3 2,112,992 + Pf1N1B4_1839 0.60 -1.3 2,112,993 - Pf1N1B4_1839 0.60 -2.3 2,112,993 - Pf1N1B4_1839 0.60 -0.5 2,112,993 - Pf1N1B4_1839 0.60 -1.8 2,112,993 - Pf1N1B4_1839 0.60 +0.4 2,113,183 + +0.0 2,113,207 + +0.2 2,113,207 + -1.3 2,113,207 + +0.6 2,113,207 + +2.5 2,113,208 + +0.3 2,113,208 - +0.0 2,113,208 - +0.2 2,113,260 + Pf1N1B4_1840 0.14 +0.0 2,113,260 + Pf1N1B4_1840 0.14 -1.5 2,113,261 - Pf1N1B4_1840 0.14 -0.3 2,113,261 - Pf1N1B4_1840 0.14 +0.0 2,113,261 - Pf1N1B4_1840 0.14 +0.8 2,113,261 - Pf1N1B4_1840 0.14 -0.8 2,113,261 - Pf1N1B4_1840 0.14 +1.2 2,113,261 - Pf1N1B4_1840 0.14 -0.8 2,113,288 + Pf1N1B4_1840 0.21 -1.1 2,113,290 + Pf1N1B4_1840 0.21 +0.8 2,113,290 + Pf1N1B4_1840 0.21 -1.2 2,113,291 - Pf1N1B4_1840 0.21 -2.5 2,113,291 - Pf1N1B4_1840 0.21 -1.3 2,113,413 + Pf1N1B4_1840 0.51 -0.8 2,113,414 - Pf1N1B4_1840 0.51 +0.6 2,113,414 - Pf1N1B4_1840 0.51 +0.4 2,113,414 - Pf1N1B4_1840 0.51 -1.1 2,113,414 - Pf1N1B4_1840 0.51 +1.0 2,113,445 + Pf1N1B4_1840 0.58 +0.2 2,113,445 + Pf1N1B4_1840 0.58 -0.1 2,113,446 - Pf1N1B4_1840 0.59 +0.2 2,113,446 - Pf1N1B4_1840 0.59 -0.5 2,113,455 + Pf1N1B4_1840 0.61 +0.9 2,113,455 + Pf1N1B4_1840 0.61 -0.1 2,113,456 - Pf1N1B4_1840 0.61 -0.1 2,113,490 + Pf1N1B4_1840 0.69 +0.2 2,113,491 - Pf1N1B4_1840 0.69 +1.2 2,113,491 - Pf1N1B4_1840 0.69 +0.2 2,113,558 + Pf1N1B4_1840 0.85 -0.2 2,113,558 + Pf1N1B4_1840 0.85 +1.2 2,113,559 - Pf1N1B4_1840 0.86 -2.2 2,113,559 - Pf1N1B4_1840 0.86 +1.7 2,113,559 - Pf1N1B4_1840 0.86 -1.6 2,113,562 + Pf1N1B4_1840 0.86 -1.1 2,113,562 + Pf1N1B4_1840 0.86 +1.5 2,113,562 + Pf1N1B4_1840 0.86 +0.7 2,113,563 - Pf1N1B4_1840 0.87 -0.3 2,113,563 - Pf1N1B4_1840 0.87 +1.2 2,113,563 - Pf1N1B4_1840 0.87 -0.4 2,113,619 - +0.6 2,113,642 - -1.1 2,113,705 + +1.2 2,113,705 + +0.1 2,113,706 - -1.1 2,113,706 - +1.4 2,113,721 - -0.1 2,113,754 - -1.6 2,113,779 + -0.1 2,113,779 + +0.1 2,113,779 + +0.3 2,113,779 + +0.7 2,113,779 + +0.3 2,113,779 + -0.9 2,113,780 - -0.1 2,113,780 - +0.4 2,113,780 - -1.9 2,113,780 - +1.2 2,113,780 - +1.0 2,113,780 - +0.8 2,113,780 - +0.4 2,113,780 - -1.1 2,113,780 - -0.1 2,113,782 + +0.2 2,113,782 + +0.0 2,113,782 + -0.6 2,113,959 + Pf1N1B4_1841 0.21 -0.8 2,113,959 + Pf1N1B4_1841 0.21 -0.3 2,113,984 + Pf1N1B4_1841 0.24 +0.1 2,113,984 + -0.3 2,113,985 - Pf1N1B4_1841 0.24 +0.7 2,113,985 - Pf1N1B4_1841 0.24 -0.7 2,113,985 - Pf1N1B4_1841 0.24 +0.2 2,113,985 - Pf1N1B4_1841 0.24 -0.6 2,114,076 + Pf1N1B4_1841 0.33 +0.9 2,114,076 + Pf1N1B4_1841 0.33 +0.4 2,114,076 + Pf1N1B4_1841 0.33 +0.0 2,114,076 + Pf1N1B4_1841 0.33 -1.6 2,114,076 + Pf1N1B4_1841 0.33 +2.1 2,114,077 - Pf1N1B4_1841 0.33 -0.3 2,114,077 - Pf1N1B4_1841 0.33 +1.2 2,114,077 - Pf1N1B4_1841 0.33 -0.1 2,114,077 - Pf1N1B4_1841 0.33 -0.6 2,114,077 - Pf1N1B4_1841 0.33 -0.3 2,114,126 + Pf1N1B4_1841 0.38 +0.5 2,114,126 + Pf1N1B4_1841 0.38 +1.4 2,114,126 + Pf1N1B4_1841 0.38 -0.3 2,114,127 - Pf1N1B4_1841 0.38 -0.9 2,114,127 - Pf1N1B4_1841 0.38 -0.2 2,114,127 - Pf1N1B4_1841 0.38 -1.9 2,114,127 - Pf1N1B4_1841 0.38 -2.8 2,114,157 + Pf1N1B4_1841 0.41 +0.4 2,114,158 - Pf1N1B4_1841 0.41 +0.9 2,114,158 - Pf1N1B4_1841 0.41 -0.7
Or see this region's nucleotide sequence