Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1769 and Pf1N1B4_1770 are separated by 268 nucleotides Pf1N1B4_1770 and Pf1N1B4_1771 overlap by 1 nucleotides Pf1N1B4_1771 and Pf1N1B4_1772 are separated by 361 nucleotides
Pf1N1B4_1769 - Glutamate--cysteine ligase (EC 6.3.2.2), at 2,021,931 to 2,023,514
_1769
Pf1N1B4_1770 - Uncharacterized protein, possibly involved in aromatic compounds catabolism, at 2,023,783 to 2,024,166
_1770
Pf1N1B4_1771 - Transcription accessory protein (S1 RNA-binding domain), at 2,024,166 to 2,026,490
_1771
Pf1N1B4_1772 - Two-component system response regulator OmpR, at 2,026,852 to 2,027,592
_1772
Position (kb)
2024
2025
2026
2027 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2023.568 kb on + strand at 2023.568 kb on + strand at 2023.569 kb on - strand at 2023.582 kb on + strand at 2023.682 kb on + strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.749 kb on - strand at 2023.776 kb on + strand at 2023.776 kb on + strand at 2023.777 kb on - strand at 2023.777 kb on - strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.784 kb on + strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2025.089 kb on - strand, within Pf1N1B4_1771 at 2025.229 kb on + strand, within Pf1N1B4_1771 at 2025.229 kb on + strand, within Pf1N1B4_1771 at 2025.229 kb on + strand, within Pf1N1B4_1771 at 2025.230 kb on - strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.949 kb on + strand, within Pf1N1B4_1771 at 2025.949 kb on + strand, within Pf1N1B4_1771 at 2025.949 kb on + strand, within Pf1N1B4_1771 at 2025.950 kb on - strand, within Pf1N1B4_1771 at 2025.950 kb on - strand at 2025.953 kb on + strand, within Pf1N1B4_1771 at 2025.953 kb on + strand, within Pf1N1B4_1771 at 2025.954 kb on - strand, within Pf1N1B4_1771 at 2026.006 kb on + strand at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.177 kb on + strand, within Pf1N1B4_1771 at 2026.178 kb on - strand, within Pf1N1B4_1771 at 2026.178 kb on - strand, within Pf1N1B4_1771 at 2026.178 kb on - strand, within Pf1N1B4_1771 at 2026.186 kb on + strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.312 kb on + strand at 2026.400 kb on + strand at 2026.401 kb on - strand at 2026.450 kb on - strand at 2026.536 kb on + strand at 2026.536 kb on + strand at 2026.536 kb on + strand at 2026.536 kb on + strand at 2026.537 kb on - strand at 2026.625 kb on - strand at 2026.625 kb on - strand at 2026.625 kb on - strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.702 kb on - strand at 2026.702 kb on - strand at 2026.703 kb on + strand at 2026.703 kb on + strand at 2026.703 kb on + strand at 2026.704 kb on - strand at 2026.704 kb on - strand at 2026.741 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.815 kb on + strand at 2026.815 kb on + strand at 2026.822 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 2,023,568 + +0.0 2,023,568 + +0.6 2,023,569 - -0.1 2,023,582 + +1.2 2,023,682 + +0.2 2,023,697 - +2.2 2,023,697 - -1.6 2,023,697 - +0.2 2,023,697 - +1.1 2,023,749 - -0.0 2,023,776 + +0.1 2,023,776 + -0.3 2,023,777 - -0.9 2,023,777 - -1.2 2,023,780 + +1.2 2,023,780 + +0.4 2,023,780 + +2.1 2,023,780 + +1.0 2,023,780 + +1.7 2,023,780 + -0.5 2,023,781 - -0.6 2,023,781 - -1.6 2,023,781 - +0.0 2,023,781 - -0.3 2,023,781 - +0.9 2,023,784 + +0.2 2,023,785 - -1.1 2,023,785 - +0.7 2,023,785 - +2.2 2,023,785 - +1.6 2,023,785 - -0.2 2,023,785 - -1.5 2,023,925 + Pf1N1B4_1770 0.37 +0.2 2,023,925 + Pf1N1B4_1770 0.37 -0.2 2,023,925 + Pf1N1B4_1770 0.37 -0.9 2,023,925 + Pf1N1B4_1770 0.37 -1.1 2,023,925 + Pf1N1B4_1770 0.37 +0.4 2,023,925 + Pf1N1B4_1770 0.37 +0.2 2,023,925 + +1.2 2,023,925 + Pf1N1B4_1770 0.37 -0.3 2,023,926 - Pf1N1B4_1770 0.37 +0.2 2,023,926 - Pf1N1B4_1770 0.37 -1.1 2,023,926 - Pf1N1B4_1770 0.37 +1.4 2,023,926 - Pf1N1B4_1770 0.37 -0.1 2,023,926 - Pf1N1B4_1770 0.37 -0.3 2,023,926 - Pf1N1B4_1770 0.37 -0.8 2,023,926 - Pf1N1B4_1770 0.37 -0.4 2,024,459 + Pf1N1B4_1771 0.13 -1.1 2,024,459 + Pf1N1B4_1771 0.13 +0.3 2,024,459 + Pf1N1B4_1771 0.13 -1.3 2,024,459 + Pf1N1B4_1771 0.13 -1.3 2,024,459 + Pf1N1B4_1771 0.13 +0.2 2,024,459 + Pf1N1B4_1771 0.13 -1.6 2,024,459 + Pf1N1B4_1771 0.13 -0.3 2,024,459 + Pf1N1B4_1771 0.13 -2.1 2,024,459 + Pf1N1B4_1771 0.13 +0.2 2,024,459 + Pf1N1B4_1771 0.13 -0.5 2,024,459 + Pf1N1B4_1771 0.13 -1.3 2,024,459 + Pf1N1B4_1771 0.13 +0.4 2,024,459 + Pf1N1B4_1771 0.13 -0.6 2,024,459 + Pf1N1B4_1771 0.13 +0.7 2,024,460 - Pf1N1B4_1771 0.13 -0.3 2,024,460 - Pf1N1B4_1771 0.13 -1.2 2,024,460 - Pf1N1B4_1771 0.13 -2.0 2,024,460 - Pf1N1B4_1771 0.13 +0.5 2,024,460 - Pf1N1B4_1771 0.13 -0.8 2,024,460 - Pf1N1B4_1771 0.13 -1.9 2,024,460 - Pf1N1B4_1771 0.13 +0.9 2,024,460 - Pf1N1B4_1771 0.13 -1.3 2,024,460 - Pf1N1B4_1771 0.13 -0.6 2,024,460 - -1.3 2,024,460 - Pf1N1B4_1771 0.13 +1.0 2,024,460 - +0.2 2,024,460 - Pf1N1B4_1771 0.13 -1.6 2,024,460 - Pf1N1B4_1771 0.13 +1.2 2,024,460 - Pf1N1B4_1771 0.13 +0.5 2,024,460 - Pf1N1B4_1771 0.13 -1.1 2,024,460 - +1.8 2,024,463 + Pf1N1B4_1771 0.13 -0.9 2,024,463 + Pf1N1B4_1771 0.13 +0.1 2,024,463 + Pf1N1B4_1771 0.13 +0.5 2,024,463 + Pf1N1B4_1771 0.13 +0.6 2,024,463 + Pf1N1B4_1771 0.13 -0.6 2,024,464 - Pf1N1B4_1771 0.13 +0.8 2,024,464 - Pf1N1B4_1771 0.13 -0.8 2,024,464 - Pf1N1B4_1771 0.13 -0.4 2,024,683 + Pf1N1B4_1771 0.22 -1.1 2,024,683 + Pf1N1B4_1771 0.22 -1.2 2,024,683 + Pf1N1B4_1771 0.22 -0.6 2,024,683 + Pf1N1B4_1771 0.22 -0.4 2,024,683 + Pf1N1B4_1771 0.22 -0.1 2,024,684 - Pf1N1B4_1771 0.22 -2.5 2,024,684 - Pf1N1B4_1771 0.22 -0.9 2,024,684 - Pf1N1B4_1771 0.22 -1.6 2,024,829 + Pf1N1B4_1771 0.29 -0.6 2,024,829 + Pf1N1B4_1771 0.29 -0.4 2,024,829 + Pf1N1B4_1771 0.29 -0.5 2,025,089 - Pf1N1B4_1771 0.40 -1.9 2,025,229 + Pf1N1B4_1771 0.46 +0.2 2,025,229 + Pf1N1B4_1771 0.46 +1.2 2,025,229 + Pf1N1B4_1771 0.46 -0.5 2,025,230 - Pf1N1B4_1771 0.46 +0.0 2,025,640 + Pf1N1B4_1771 0.63 +0.2 2,025,640 + Pf1N1B4_1771 0.63 +0.9 2,025,640 + Pf1N1B4_1771 0.63 -1.0 2,025,640 + Pf1N1B4_1771 0.63 -1.4 2,025,640 + Pf1N1B4_1771 0.63 -1.2 2,025,641 - Pf1N1B4_1771 0.63 +0.0 2,025,641 - Pf1N1B4_1771 0.63 -0.9 2,025,641 - Pf1N1B4_1771 0.63 -0.1 2,025,641 - Pf1N1B4_1771 0.63 -0.5 2,025,641 - Pf1N1B4_1771 0.63 -2.2 2,025,949 + Pf1N1B4_1771 0.77 -2.2 2,025,949 + Pf1N1B4_1771 0.77 -2.1 2,025,949 + Pf1N1B4_1771 0.77 +0.4 2,025,950 - Pf1N1B4_1771 0.77 -2.0 2,025,950 - +1.2 2,025,953 + Pf1N1B4_1771 0.77 +1.2 2,025,953 + Pf1N1B4_1771 0.77 -0.1 2,025,954 - Pf1N1B4_1771 0.77 -1.9 2,026,006 + +0.5 2,026,006 + Pf1N1B4_1771 0.79 -0.4 2,026,006 + Pf1N1B4_1771 0.79 -1.1 2,026,006 + Pf1N1B4_1771 0.79 -2.1 2,026,006 + +0.2 2,026,006 + Pf1N1B4_1771 0.79 -1.1 2,026,006 + Pf1N1B4_1771 0.79 -0.5 2,026,007 - Pf1N1B4_1771 0.79 -0.4 2,026,007 - +1.8 2,026,007 - Pf1N1B4_1771 0.79 -1.2 2,026,007 - Pf1N1B4_1771 0.79 -2.2 2,026,007 - Pf1N1B4_1771 0.79 +0.2 2,026,007 - Pf1N1B4_1771 0.79 -1.8 2,026,007 - Pf1N1B4_1771 0.79 +1.2 2,026,007 - Pf1N1B4_1771 0.79 -0.8 2,026,177 + Pf1N1B4_1771 0.86 -0.9 2,026,178 - Pf1N1B4_1771 0.87 -0.1 2,026,178 - Pf1N1B4_1771 0.87 +0.5 2,026,178 - Pf1N1B4_1771 0.87 -0.9 2,026,186 + Pf1N1B4_1771 0.87 -2.1 2,026,187 - Pf1N1B4_1771 0.87 -0.8 2,026,187 - Pf1N1B4_1771 0.87 -0.0 2,026,187 - Pf1N1B4_1771 0.87 -1.6 2,026,187 - Pf1N1B4_1771 0.87 +0.2 2,026,187 - Pf1N1B4_1771 0.87 -0.4 2,026,312 + -0.1 2,026,400 + -1.6 2,026,401 - -1.7 2,026,450 - +1.0 2,026,536 + -1.5 2,026,536 + +1.2 2,026,536 + -1.6 2,026,536 + -2.2 2,026,537 - -0.7 2,026,625 - +2.1 2,026,625 - -0.5 2,026,625 - -0.8 2,026,658 + +1.2 2,026,658 + +0.2 2,026,658 + -0.2 2,026,658 + -0.3 2,026,658 + +0.9 2,026,658 + -1.4 2,026,701 + -1.5 2,026,701 + +0.9 2,026,701 + +0.2 2,026,701 + -2.2 2,026,701 + -0.1 2,026,701 + -0.4 2,026,701 + +0.0 2,026,701 + -0.5 2,026,702 - +1.6 2,026,702 - -0.6 2,026,703 + -0.0 2,026,703 + -0.3 2,026,703 + +3.0 2,026,704 - -0.5 2,026,704 - -0.8 2,026,741 + +2.1 2,026,803 + -0.4 2,026,803 + -0.4 2,026,803 + +1.6 2,026,803 + +0.6 2,026,803 + -0.8 2,026,815 + +1.6 2,026,815 + -0.8 2,026,822 + +0.4
Or see this region's nucleotide sequence