Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1744

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1743 and Pf1N1B4_1744 are separated by 42 nucleotidesPf1N1B4_1744 and Pf1N1B4_1745 are separated by 12 nucleotides Pf1N1B4_1743 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 1,992,063 to 1,993,418 _1743 Pf1N1B4_1744 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 1,993,461 to 1,995,416 _1744 Pf1N1B4_1745 - Biofilm PGA outer membrane secretin PgaA, at 1,995,429 to 1,997,909 _1745 Position (kb) 1993 1994 1995 1996Strain fitness (log2 ratio) -2 -1 0 1 2at 1992.895 kb on + strand, within Pf1N1B4_1743at 1992.896 kb on - strand, within Pf1N1B4_1743at 1992.896 kb on - strand, within Pf1N1B4_1743at 1993.168 kb on + strand, within Pf1N1B4_1743at 1993.168 kb on + strand, within Pf1N1B4_1743at 1993.168 kb on + strand, within Pf1N1B4_1743at 1993.168 kb on + strand, within Pf1N1B4_1743at 1993.168 kb on + strand, within Pf1N1B4_1743at 1993.169 kb on - strand, within Pf1N1B4_1743at 1993.169 kb on - strand, within Pf1N1B4_1743at 1993.169 kb on - strand, within Pf1N1B4_1743at 1993.169 kb on - strand, within Pf1N1B4_1743at 1993.223 kb on + strand, within Pf1N1B4_1743at 1993.224 kb on - strand, within Pf1N1B4_1743at 1993.224 kb on - strand, within Pf1N1B4_1743at 1993.224 kb on - strand, within Pf1N1B4_1743at 1993.267 kb on + strand, within Pf1N1B4_1743at 1993.268 kb on - strand, within Pf1N1B4_1743at 1993.401 kb on + strandat 1993.402 kb on - strandat 1993.421 kb on + strandat 1993.421 kb on + strandat 1993.500 kb on - strandat 1993.737 kb on - strand, within Pf1N1B4_1744at 1993.827 kb on + strand, within Pf1N1B4_1744at 1993.827 kb on + strand, within Pf1N1B4_1744at 1993.827 kb on + strand, within Pf1N1B4_1744at 1993.828 kb on - strand, within Pf1N1B4_1744at 1993.975 kb on + strand, within Pf1N1B4_1744at 1994.036 kb on - strand, within Pf1N1B4_1744at 1994.036 kb on - strand, within Pf1N1B4_1744at 1994.077 kb on + strand, within Pf1N1B4_1744at 1994.077 kb on + strand, within Pf1N1B4_1744at 1994.131 kb on + strand, within Pf1N1B4_1744at 1994.131 kb on + strand, within Pf1N1B4_1744at 1994.132 kb on - strand, within Pf1N1B4_1744at 1994.233 kb on + strand, within Pf1N1B4_1744at 1994.233 kb on + strand, within Pf1N1B4_1744at 1994.233 kb on + strand, within Pf1N1B4_1744at 1994.285 kb on + strand, within Pf1N1B4_1744at 1994.320 kb on + strand, within Pf1N1B4_1744at 1994.321 kb on - strand, within Pf1N1B4_1744at 1994.321 kb on - strand, within Pf1N1B4_1744at 1994.321 kb on - strand, within Pf1N1B4_1744at 1994.698 kb on + strand, within Pf1N1B4_1744at 1994.779 kb on + strand, within Pf1N1B4_1744at 1994.958 kb on - strand, within Pf1N1B4_1744at 1995.032 kb on + strand, within Pf1N1B4_1744at 1995.032 kb on + strand, within Pf1N1B4_1744at 1995.032 kb on + strand, within Pf1N1B4_1744at 1995.032 kb on + strand, within Pf1N1B4_1744at 1995.033 kb on - strand, within Pf1N1B4_1744at 1995.033 kb on - strand, within Pf1N1B4_1744at 1995.034 kb on + strand, within Pf1N1B4_1744at 1995.035 kb on - strand, within Pf1N1B4_1744at 1995.064 kb on + strand, within Pf1N1B4_1744at 1995.064 kb on + strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.065 kb on - strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.169 kb on + strand, within Pf1N1B4_1744at 1995.170 kb on - strand, within Pf1N1B4_1744at 1995.170 kb on - strand, within Pf1N1B4_1744at 1995.170 kb on - strand, within Pf1N1B4_1744at 1995.170 kb on - strand, within Pf1N1B4_1744at 1995.170 kb on - strand, within Pf1N1B4_1744at 1995.170 kb on - strand, within Pf1N1B4_1744at 1995.279 kb on + strandat 1995.280 kb on - strandat 1995.280 kb on - strandat 1995.280 kb on - strandat 1995.407 kb on + strandat 1995.407 kb on + strandat 1995.407 kb on + strandat 1995.408 kb on - strandat 1995.408 kb on - strandat 1995.408 kb on - strandat 1995.408 kb on - strandat 1995.430 kb on + strandat 1995.431 kb on - strandat 1995.439 kb on + strandat 1995.439 kb on + strandat 1995.440 kb on - strandat 1995.440 kb on - strandat 1995.440 kb on - strandat 1995.487 kb on + strandat 1995.528 kb on + strandat 1995.528 kb on + strandat 1995.528 kb on + strandat 1995.528 kb on + strandat 1995.528 kb on + strandat 1995.528 kb on + strandat 1995.529 kb on - strandat 1995.529 kb on - strandat 1995.599 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.601 kb on + strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.602 kb on - strandat 1995.646 kb on + strandat 1995.656 kb on - strandat 1995.741 kb on - strand, within Pf1N1B4_1745at 1995.741 kb on - strand, within Pf1N1B4_1745at 1995.741 kb on - strand, within Pf1N1B4_1745at 1995.973 kb on + strand, within Pf1N1B4_1745at 1995.974 kb on - strandat 1995.974 kb on - strand, within Pf1N1B4_1745at 1996.058 kb on - strand, within Pf1N1B4_1745at 1996.058 kb on - strand, within Pf1N1B4_1745at 1996.110 kb on + strand, within Pf1N1B4_1745at 1996.206 kb on - strand, within Pf1N1B4_1745at 1996.216 kb on + strand, within Pf1N1B4_1745at 1996.216 kb on + strand, within Pf1N1B4_1745at 1996.217 kb on - strand, within Pf1N1B4_1745at 1996.217 kb on - strand, within Pf1N1B4_1745at 1996.217 kb on - strand, within Pf1N1B4_1745at 1996.285 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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1,992,895 + Pf1N1B4_1743 0.61 -1.1
1,992,896 - Pf1N1B4_1743 0.61 -1.2
1,992,896 - Pf1N1B4_1743 0.61 +0.1
1,993,168 + Pf1N1B4_1743 0.81 -0.2
1,993,168 + Pf1N1B4_1743 0.81 -0.3
1,993,168 + Pf1N1B4_1743 0.81 -1.1
1,993,168 + Pf1N1B4_1743 0.81 +0.2
1,993,168 + Pf1N1B4_1743 0.81 +0.2
1,993,169 - Pf1N1B4_1743 0.82 -0.3
1,993,169 - Pf1N1B4_1743 0.82 -1.1
1,993,169 - Pf1N1B4_1743 0.82 -0.6
1,993,169 - Pf1N1B4_1743 0.82 +0.3
1,993,223 + Pf1N1B4_1743 0.86 -1.6
1,993,224 - Pf1N1B4_1743 0.86 +0.3
1,993,224 - Pf1N1B4_1743 0.86 -1.3
1,993,224 - Pf1N1B4_1743 0.86 +0.4
1,993,267 + Pf1N1B4_1743 0.89 +0.8
1,993,268 - Pf1N1B4_1743 0.89 +0.4
1,993,401 + +1.4
1,993,402 - -0.1
1,993,421 + -0.3
1,993,421 + +1.0
1,993,500 - -0.1
1,993,737 - Pf1N1B4_1744 0.14 +1.6
1,993,827 + Pf1N1B4_1744 0.19 +1.2
1,993,827 + Pf1N1B4_1744 0.19 -0.6
1,993,827 + Pf1N1B4_1744 0.19 -0.9
1,993,828 - Pf1N1B4_1744 0.19 +0.2
1,993,975 + Pf1N1B4_1744 0.26 +0.8
1,994,036 - Pf1N1B4_1744 0.29 +1.6
1,994,036 - Pf1N1B4_1744 0.29 -2.1
1,994,077 + Pf1N1B4_1744 0.31 +0.4
1,994,077 + Pf1N1B4_1744 0.31 +0.7
1,994,131 + Pf1N1B4_1744 0.34 -1.8
1,994,131 + Pf1N1B4_1744 0.34 -0.3
1,994,132 - Pf1N1B4_1744 0.34 -0.1
1,994,233 + Pf1N1B4_1744 0.39 +1.7
1,994,233 + Pf1N1B4_1744 0.39 +1.2
1,994,233 + Pf1N1B4_1744 0.39 -0.8
1,994,285 + Pf1N1B4_1744 0.42 -0.8
1,994,320 + Pf1N1B4_1744 0.44 +0.5
1,994,321 - Pf1N1B4_1744 0.44 +0.2
1,994,321 - Pf1N1B4_1744 0.44 +0.7
1,994,321 - Pf1N1B4_1744 0.44 +1.5
1,994,698 + Pf1N1B4_1744 0.63 -1.3
1,994,779 + Pf1N1B4_1744 0.67 +0.8
1,994,958 - Pf1N1B4_1744 0.77 +1.2
1,995,032 + Pf1N1B4_1744 0.80 +0.5
1,995,032 + Pf1N1B4_1744 0.80 +2.6
1,995,032 + Pf1N1B4_1744 0.80 -1.8
1,995,032 + Pf1N1B4_1744 0.80 +0.4
1,995,033 - Pf1N1B4_1744 0.80 -0.2
1,995,033 - Pf1N1B4_1744 0.80 -0.6
1,995,034 + Pf1N1B4_1744 0.80 +0.9
1,995,035 - Pf1N1B4_1744 0.80 +0.2
1,995,064 + Pf1N1B4_1744 0.82 -0.5
1,995,064 + Pf1N1B4_1744 0.82 -0.0
1,995,065 - Pf1N1B4_1744 0.82 -1.3
1,995,065 - Pf1N1B4_1744 0.82 -1.1
1,995,065 - Pf1N1B4_1744 0.82 -1.0
1,995,065 - Pf1N1B4_1744 0.82 -1.1
1,995,065 - Pf1N1B4_1744 0.82 -0.1
1,995,065 - Pf1N1B4_1744 0.82 -1.6
1,995,065 - Pf1N1B4_1744 0.82 -0.6
1,995,065 - Pf1N1B4_1744 0.82 -1.9
1,995,169 + Pf1N1B4_1744 0.87 +1.2
1,995,169 + Pf1N1B4_1744 0.87 +0.5
1,995,169 + Pf1N1B4_1744 0.87 -0.6
1,995,169 + Pf1N1B4_1744 0.87 +0.4
1,995,169 + Pf1N1B4_1744 0.87 +1.2
1,995,169 + Pf1N1B4_1744 0.87 +0.7
1,995,169 + Pf1N1B4_1744 0.87 +0.9
1,995,170 - Pf1N1B4_1744 0.87 +0.7
1,995,170 - Pf1N1B4_1744 0.87 -0.5
1,995,170 - Pf1N1B4_1744 0.87 -1.2
1,995,170 - Pf1N1B4_1744 0.87 +0.7
1,995,170 - Pf1N1B4_1744 0.87 -1.6
1,995,170 - Pf1N1B4_1744 0.87 +0.0
1,995,279 + +0.9
1,995,280 - -0.6
1,995,280 - -0.3
1,995,280 - +2.2
1,995,407 + +0.0
1,995,407 + -0.6
1,995,407 + +1.2
1,995,408 - -1.8
1,995,408 - +0.9
1,995,408 - -0.5
1,995,408 - -0.2
1,995,430 + -1.1
1,995,431 - +0.4
1,995,439 + -2.1
1,995,439 + -1.1
1,995,440 - -0.1
1,995,440 - -1.9
1,995,440 - +0.9
1,995,487 + -0.3
1,995,528 + +0.1
1,995,528 + +0.8
1,995,528 + +0.1
1,995,528 + -0.8
1,995,528 + +0.2
1,995,528 + +0.7
1,995,529 - +1.2
1,995,529 - -0.9
1,995,599 + +0.2
1,995,601 + -0.1
1,995,601 + +0.2
1,995,601 + +0.0
1,995,601 + +0.6
1,995,601 + +0.0
1,995,601 + -0.1
1,995,601 + +0.4
1,995,601 + +2.2
1,995,601 + +0.9
1,995,601 + +1.2
1,995,602 - -0.8
1,995,602 - +0.2
1,995,602 - -1.9
1,995,602 - -0.1
1,995,602 - -1.8
1,995,602 - -1.1
1,995,602 - +1.6
1,995,602 - -1.1
1,995,646 + +0.2
1,995,656 - +1.5
1,995,741 - Pf1N1B4_1745 0.13 +0.7
1,995,741 - Pf1N1B4_1745 0.13 -0.1
1,995,741 - Pf1N1B4_1745 0.13 +1.2
1,995,973 + Pf1N1B4_1745 0.22 -0.8
1,995,974 - +1.2
1,995,974 - Pf1N1B4_1745 0.22 +1.0
1,996,058 - Pf1N1B4_1745 0.25 +1.2
1,996,058 - Pf1N1B4_1745 0.25 +0.4
1,996,110 + Pf1N1B4_1745 0.27 -1.9
1,996,206 - Pf1N1B4_1745 0.31 +1.0
1,996,216 + Pf1N1B4_1745 0.32 -0.6
1,996,216 + Pf1N1B4_1745 0.32 -0.1
1,996,217 - Pf1N1B4_1745 0.32 -0.0
1,996,217 - Pf1N1B4_1745 0.32 -0.6
1,996,217 - Pf1N1B4_1745 0.32 -0.2
1,996,285 + +2.2

Or see this region's nucleotide sequence