Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1571 and Pf1N1B4_1572 are separated by 144 nucleotides Pf1N1B4_1572 and Pf1N1B4_1573 overlap by 8 nucleotides
Pf1N1B4_1571 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,806,851 to 1,808,767
_1571
Pf1N1B4_1572 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 1,808,912 to 1,811,482
_1572
Pf1N1B4_1573 - Glucans biosynthesis protein G precursor, at 1,811,475 to 1,813,250
_1573
Position (kb)
1808
1809
1810
1811
1812 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1809.270 kb on + strand, within Pf1N1B4_1572 at 1809.271 kb on - strand, within Pf1N1B4_1572 at 1809.306 kb on + strand, within Pf1N1B4_1572 at 1809.306 kb on + strand, within Pf1N1B4_1572 at 1809.307 kb on - strand, within Pf1N1B4_1572 at 1809.804 kb on + strand at 1809.805 kb on - strand, within Pf1N1B4_1572 at 1809.828 kb on + strand, within Pf1N1B4_1572 at 1809.829 kb on - strand, within Pf1N1B4_1572 at 1809.829 kb on - strand, within Pf1N1B4_1572 at 1809.829 kb on - strand, within Pf1N1B4_1572 at 1809.829 kb on - strand, within Pf1N1B4_1572 at 1809.829 kb on - strand, within Pf1N1B4_1572 at 1809.848 kb on - strand, within Pf1N1B4_1572 at 1809.848 kb on - strand, within Pf1N1B4_1572 at 1809.909 kb on + strand, within Pf1N1B4_1572 at 1809.909 kb on + strand, within Pf1N1B4_1572 at 1809.910 kb on - strand, within Pf1N1B4_1572 at 1809.910 kb on - strand, within Pf1N1B4_1572 at 1809.963 kb on - strand, within Pf1N1B4_1572 at 1809.963 kb on - strand, within Pf1N1B4_1572 at 1810.044 kb on - strand, within Pf1N1B4_1572 at 1810.044 kb on - strand, within Pf1N1B4_1572 at 1810.054 kb on - strand, within Pf1N1B4_1572 at 1810.284 kb on + strand, within Pf1N1B4_1572 at 1810.284 kb on + strand, within Pf1N1B4_1572 at 1810.284 kb on + strand, within Pf1N1B4_1572 at 1810.285 kb on - strand, within Pf1N1B4_1572 at 1810.285 kb on - strand, within Pf1N1B4_1572 at 1810.285 kb on - strand at 1810.285 kb on - strand, within Pf1N1B4_1572 at 1810.318 kb on - strand, within Pf1N1B4_1572 at 1810.318 kb on - strand, within Pf1N1B4_1572 at 1810.449 kb on + strand, within Pf1N1B4_1572 at 1810.449 kb on + strand, within Pf1N1B4_1572 at 1810.449 kb on + strand, within Pf1N1B4_1572 at 1810.449 kb on + strand, within Pf1N1B4_1572 at 1810.449 kb on + strand, within Pf1N1B4_1572 at 1810.449 kb on + strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.450 kb on - strand, within Pf1N1B4_1572 at 1810.455 kb on + strand, within Pf1N1B4_1572 at 1810.455 kb on + strand, within Pf1N1B4_1572 at 1810.455 kb on + strand, within Pf1N1B4_1572 at 1810.455 kb on + strand, within Pf1N1B4_1572 at 1810.455 kb on + strand, within Pf1N1B4_1572 at 1810.456 kb on - strand, within Pf1N1B4_1572 at 1810.456 kb on - strand, within Pf1N1B4_1572 at 1810.456 kb on - strand, within Pf1N1B4_1572 at 1810.501 kb on + strand, within Pf1N1B4_1572 at 1810.508 kb on + strand, within Pf1N1B4_1572 at 1810.509 kb on - strand, within Pf1N1B4_1572 at 1810.530 kb on + strand, within Pf1N1B4_1572 at 1810.794 kb on + strand, within Pf1N1B4_1572 at 1810.794 kb on + strand, within Pf1N1B4_1572 at 1810.882 kb on + strand, within Pf1N1B4_1572 at 1810.883 kb on - strand, within Pf1N1B4_1572 at 1810.883 kb on - strand, within Pf1N1B4_1572 at 1810.909 kb on - strand, within Pf1N1B4_1572 at 1810.909 kb on - strand, within Pf1N1B4_1572 at 1810.909 kb on - strand, within Pf1N1B4_1572 at 1811.007 kb on + strand, within Pf1N1B4_1572 at 1811.007 kb on + strand, within Pf1N1B4_1572 at 1811.007 kb on + strand, within Pf1N1B4_1572 at 1811.008 kb on - strand, within Pf1N1B4_1572 at 1811.055 kb on + strand, within Pf1N1B4_1572 at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.477 kb on - strand at 1811.477 kb on - strand at 1811.498 kb on - strand at 1811.498 kb on - strand at 1811.956 kb on + strand, within Pf1N1B4_1573 at 1811.957 kb on - strand, within Pf1N1B4_1573 at 1811.957 kb on - strand, within Pf1N1B4_1573 at 1812.149 kb on + strand, within Pf1N1B4_1573 at 1812.150 kb on - strand, within Pf1N1B4_1573 at 1812.271 kb on + strand, within Pf1N1B4_1573 at 1812.272 kb on - strand, within Pf1N1B4_1573 at 1812.437 kb on - strand, within Pf1N1B4_1573
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,809,270 + Pf1N1B4_1572 0.14 +0.4 1,809,271 - Pf1N1B4_1572 0.14 +2.5 1,809,306 + Pf1N1B4_1572 0.15 +0.2 1,809,306 + Pf1N1B4_1572 0.15 +1.3 1,809,307 - Pf1N1B4_1572 0.15 -0.8 1,809,804 + +2.8 1,809,805 - Pf1N1B4_1572 0.35 +0.6 1,809,828 + Pf1N1B4_1572 0.36 +1.7 1,809,829 - Pf1N1B4_1572 0.36 +1.0 1,809,829 - Pf1N1B4_1572 0.36 +2.4 1,809,829 - Pf1N1B4_1572 0.36 -0.2 1,809,829 - Pf1N1B4_1572 0.36 +0.7 1,809,829 - Pf1N1B4_1572 0.36 -0.4 1,809,848 - Pf1N1B4_1572 0.36 +2.2 1,809,848 - Pf1N1B4_1572 0.36 -1.1 1,809,909 + Pf1N1B4_1572 0.39 +0.6 1,809,909 + Pf1N1B4_1572 0.39 +0.7 1,809,910 - Pf1N1B4_1572 0.39 +0.2 1,809,910 - Pf1N1B4_1572 0.39 -0.8 1,809,963 - Pf1N1B4_1572 0.41 +1.7 1,809,963 - Pf1N1B4_1572 0.41 -0.1 1,810,044 - Pf1N1B4_1572 0.44 +0.9 1,810,044 - Pf1N1B4_1572 0.44 +0.7 1,810,054 - Pf1N1B4_1572 0.44 +0.1 1,810,284 + Pf1N1B4_1572 0.53 +0.2 1,810,284 + Pf1N1B4_1572 0.53 +0.9 1,810,284 + Pf1N1B4_1572 0.53 -0.4 1,810,285 - Pf1N1B4_1572 0.53 -0.3 1,810,285 - Pf1N1B4_1572 0.53 +0.2 1,810,285 - -0.8 1,810,285 - Pf1N1B4_1572 0.53 +0.9 1,810,318 - Pf1N1B4_1572 0.55 -0.1 1,810,318 - Pf1N1B4_1572 0.55 +2.8 1,810,449 + Pf1N1B4_1572 0.60 -0.4 1,810,449 + Pf1N1B4_1572 0.60 +1.6 1,810,449 + Pf1N1B4_1572 0.60 +0.6 1,810,449 + Pf1N1B4_1572 0.60 +0.8 1,810,449 + Pf1N1B4_1572 0.60 -0.6 1,810,449 + Pf1N1B4_1572 0.60 +1.6 1,810,450 - Pf1N1B4_1572 0.60 +0.7 1,810,450 - Pf1N1B4_1572 0.60 +2.1 1,810,450 - Pf1N1B4_1572 0.60 +0.7 1,810,450 - Pf1N1B4_1572 0.60 +0.2 1,810,450 - Pf1N1B4_1572 0.60 +0.6 1,810,450 - Pf1N1B4_1572 0.60 -0.6 1,810,450 - Pf1N1B4_1572 0.60 +1.2 1,810,455 + Pf1N1B4_1572 0.60 +1.7 1,810,455 + Pf1N1B4_1572 0.60 +0.5 1,810,455 + Pf1N1B4_1572 0.60 +1.7 1,810,455 + Pf1N1B4_1572 0.60 +2.6 1,810,455 + Pf1N1B4_1572 0.60 -0.1 1,810,456 - Pf1N1B4_1572 0.60 -1.1 1,810,456 - Pf1N1B4_1572 0.60 +0.2 1,810,456 - Pf1N1B4_1572 0.60 +1.9 1,810,501 + Pf1N1B4_1572 0.62 +1.3 1,810,508 + Pf1N1B4_1572 0.62 +0.8 1,810,509 - Pf1N1B4_1572 0.62 +1.2 1,810,530 + Pf1N1B4_1572 0.63 +0.9 1,810,794 + Pf1N1B4_1572 0.73 +1.2 1,810,794 + Pf1N1B4_1572 0.73 +2.0 1,810,882 + Pf1N1B4_1572 0.77 -0.6 1,810,883 - Pf1N1B4_1572 0.77 +0.8 1,810,883 - Pf1N1B4_1572 0.77 -0.6 1,810,909 - Pf1N1B4_1572 0.78 +1.9 1,810,909 - Pf1N1B4_1572 0.78 -0.8 1,810,909 - Pf1N1B4_1572 0.78 -0.1 1,811,007 + Pf1N1B4_1572 0.81 +1.2 1,811,007 + Pf1N1B4_1572 0.81 -0.4 1,811,007 + Pf1N1B4_1572 0.81 +2.5 1,811,008 - Pf1N1B4_1572 0.82 -0.4 1,811,055 + Pf1N1B4_1572 0.83 -0.1 1,811,465 + +2.2 1,811,465 + +0.8 1,811,465 + -0.4 1,811,465 + +2.2 1,811,465 + +0.8 1,811,477 - +1.8 1,811,477 - +4.4 1,811,498 - +0.2 1,811,498 - +0.4 1,811,956 + Pf1N1B4_1573 0.27 +0.2 1,811,957 - Pf1N1B4_1573 0.27 -1.8 1,811,957 - Pf1N1B4_1573 0.27 +0.6 1,812,149 + Pf1N1B4_1573 0.38 -0.5 1,812,150 - Pf1N1B4_1573 0.38 +0.7 1,812,271 + Pf1N1B4_1573 0.45 +2.2 1,812,272 - Pf1N1B4_1573 0.45 +1.6 1,812,437 - Pf1N1B4_1573 0.54 +0.1
Or see this region's nucleotide sequence