Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1569 and Pf1N1B4_1570 overlap by 4 nucleotides Pf1N1B4_1570 and Pf1N1B4_1571 are separated by 219 nucleotides Pf1N1B4_1571 and Pf1N1B4_1572 are separated by 144 nucleotides
Pf1N1B4_1569 - Twin-arginine translocation protein TatC, at 1,805,133 to 1,805,927
_1569
Pf1N1B4_1570 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 1,805,924 to 1,806,631
_1570
Pf1N1B4_1571 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,806,851 to 1,808,767
_1571
Pf1N1B4_1572 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 1,808,912 to 1,811,482
_1572
Position (kb)
1806
1807
1808
1809 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1806.081 kb on + strand, within Pf1N1B4_1570 at 1806.081 kb on + strand, within Pf1N1B4_1570 at 1806.082 kb on - strand, within Pf1N1B4_1570 at 1806.082 kb on - strand at 1806.154 kb on - strand, within Pf1N1B4_1570 at 1806.452 kb on + strand, within Pf1N1B4_1570 at 1806.452 kb on + strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.645 kb on - strand at 1806.717 kb on + strand at 1806.775 kb on + strand at 1806.784 kb on - strand at 1806.784 kb on - strand at 1806.818 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.829 kb on - strand at 1806.844 kb on + strand at 1806.844 kb on + strand at 1806.845 kb on - strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.855 kb on - strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1807.007 kb on - strand at 1807.058 kb on + strand, within Pf1N1B4_1571 at 1807.059 kb on - strand, within Pf1N1B4_1571 at 1807.206 kb on - strand, within Pf1N1B4_1571 at 1807.225 kb on + strand, within Pf1N1B4_1571 at 1807.226 kb on - strand, within Pf1N1B4_1571 at 1807.226 kb on - strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.416 kb on - strand at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.418 kb on - strand, within Pf1N1B4_1571 at 1807.680 kb on + strand, within Pf1N1B4_1571 at 1807.681 kb on - strand, within Pf1N1B4_1571 at 1809.270 kb on + strand, within Pf1N1B4_1572 at 1809.271 kb on - strand, within Pf1N1B4_1572 at 1809.306 kb on + strand, within Pf1N1B4_1572 at 1809.306 kb on + strand, within Pf1N1B4_1572 at 1809.307 kb on - strand, within Pf1N1B4_1572
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,806,081 + Pf1N1B4_1570 0.22 +1.1 1,806,081 + Pf1N1B4_1570 0.22 +1.2 1,806,082 - Pf1N1B4_1570 0.22 +0.2 1,806,082 - -1.1 1,806,154 - Pf1N1B4_1570 0.32 +1.8 1,806,452 + Pf1N1B4_1570 0.75 +1.0 1,806,452 + Pf1N1B4_1570 0.75 +0.7 1,806,453 - Pf1N1B4_1570 0.75 +1.7 1,806,453 - Pf1N1B4_1570 0.75 +0.9 1,806,453 - Pf1N1B4_1570 0.75 -0.4 1,806,453 - Pf1N1B4_1570 0.75 -0.4 1,806,453 - Pf1N1B4_1570 0.75 +1.3 1,806,453 - Pf1N1B4_1570 0.75 +0.7 1,806,645 - -1.1 1,806,717 + +0.2 1,806,775 + -0.4 1,806,784 - -0.8 1,806,784 - +2.8 1,806,818 + +0.2 1,806,828 + +1.8 1,806,828 + -1.1 1,806,828 + +1.2 1,806,828 + +0.8 1,806,828 + +2.0 1,806,829 - +0.5 1,806,844 + +0.2 1,806,844 + +1.2 1,806,845 - -0.8 1,806,854 + +1.3 1,806,854 + +0.6 1,806,854 + -0.4 1,806,854 + -0.8 1,806,855 - -0.1 1,806,885 + -2.1 1,806,885 + -1.4 1,806,885 + +0.4 1,806,885 + +0.7 1,806,886 - -1.3 1,806,886 - +0.6 1,806,886 - +0.1 1,806,886 - +2.4 1,807,007 - -0.0 1,807,058 + Pf1N1B4_1571 0.11 -0.4 1,807,059 - Pf1N1B4_1571 0.11 +1.1 1,807,206 - Pf1N1B4_1571 0.19 +0.6 1,807,225 + Pf1N1B4_1571 0.20 -0.4 1,807,226 - Pf1N1B4_1571 0.20 +0.4 1,807,226 - Pf1N1B4_1571 0.20 +1.6 1,807,415 + Pf1N1B4_1571 0.29 -0.6 1,807,415 + Pf1N1B4_1571 0.29 +0.2 1,807,415 + Pf1N1B4_1571 0.29 +0.9 1,807,415 + Pf1N1B4_1571 0.29 +1.2 1,807,415 + Pf1N1B4_1571 0.29 +1.4 1,807,415 + Pf1N1B4_1571 0.29 -2.5 1,807,416 - +3.2 1,807,416 - Pf1N1B4_1571 0.29 +0.5 1,807,416 - Pf1N1B4_1571 0.29 +0.2 1,807,416 - Pf1N1B4_1571 0.29 +1.8 1,807,416 - Pf1N1B4_1571 0.29 +0.8 1,807,416 - -0.8 1,807,416 - Pf1N1B4_1571 0.29 +2.1 1,807,416 - Pf1N1B4_1571 0.29 -0.6 1,807,416 - Pf1N1B4_1571 0.29 +1.4 1,807,416 - Pf1N1B4_1571 0.29 +1.0 1,807,418 - Pf1N1B4_1571 0.30 +2.2 1,807,680 + Pf1N1B4_1571 0.43 +1.4 1,807,681 - Pf1N1B4_1571 0.43 -0.1 1,809,270 + Pf1N1B4_1572 0.14 +0.4 1,809,271 - Pf1N1B4_1572 0.14 +2.5 1,809,306 + Pf1N1B4_1572 0.15 +0.2 1,809,306 + Pf1N1B4_1572 0.15 +1.3 1,809,307 - Pf1N1B4_1572 0.15 -0.8
Or see this region's nucleotide sequence