Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1540 and Pf1N1B4_1541 overlap by 4 nucleotides Pf1N1B4_1541 and Pf1N1B4_1542 overlap by 4 nucleotides Pf1N1B4_1542 and Pf1N1B4_1543 overlap by 1 nucleotides
Pf1N1B4_1540 - Type IV pilus biogenesis protein PilP, at 1,776,970 to 1,777,497
_1540
Pf1N1B4_1541 - Type IV pilus biogenesis protein PilO, at 1,777,494 to 1,778,117
_1541
Pf1N1B4_1542 - Type IV pilus biogenesis protein PilN, at 1,778,114 to 1,778,680
_1542
Pf1N1B4_1543 - Type IV pilus biogenesis protein PilM, at 1,778,680 to 1,779,744
_1543
Position (kb)
1778
1779 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1777.217 kb on + strand, within Pf1N1B4_1540 at 1777.217 kb on + strand, within Pf1N1B4_1540 at 1777.326 kb on + strand, within Pf1N1B4_1540 at 1777.328 kb on + strand, within Pf1N1B4_1540 at 1777.328 kb on + strand, within Pf1N1B4_1540 at 1777.328 kb on + strand, within Pf1N1B4_1540 at 1777.329 kb on - strand, within Pf1N1B4_1540 at 1777.329 kb on - strand, within Pf1N1B4_1540 at 1777.406 kb on + strand, within Pf1N1B4_1540 at 1777.519 kb on + strand at 1777.519 kb on + strand at 1777.519 kb on + strand at 1777.696 kb on + strand, within Pf1N1B4_1541 at 1777.696 kb on + strand, within Pf1N1B4_1541 at 1777.696 kb on + strand, within Pf1N1B4_1541 at 1777.697 kb on - strand at 1777.862 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1778.120 kb on - strand at 1778.341 kb on - strand, within Pf1N1B4_1542 at 1778.514 kb on + strand at 1778.514 kb on + strand, within Pf1N1B4_1542 at 1778.514 kb on + strand, within Pf1N1B4_1542 at 1778.514 kb on + strand, within Pf1N1B4_1542 at 1778.514 kb on + strand, within Pf1N1B4_1542 at 1778.515 kb on - strand, within Pf1N1B4_1542 at 1778.615 kb on + strand, within Pf1N1B4_1542 at 1778.616 kb on - strand, within Pf1N1B4_1542 at 1778.660 kb on + strand at 1778.661 kb on - strand at 1778.891 kb on + strand, within Pf1N1B4_1543 at 1778.892 kb on - strand, within Pf1N1B4_1543 at 1778.892 kb on - strand, within Pf1N1B4_1543 at 1778.979 kb on - strand, within Pf1N1B4_1543 at 1779.038 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.096 kb on - strand, within Pf1N1B4_1543 at 1779.096 kb on - strand, within Pf1N1B4_1543 at 1779.227 kb on + strand, within Pf1N1B4_1543 at 1779.228 kb on - strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.405 kb on - strand, within Pf1N1B4_1543 at 1779.405 kb on - strand, within Pf1N1B4_1543 at 1779.585 kb on + strand, within Pf1N1B4_1543 at 1779.629 kb on + strand, within Pf1N1B4_1543 at 1779.630 kb on - strand, within Pf1N1B4_1543 at 1779.664 kb on + strand at 1779.664 kb on + strand at 1779.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,777,217 + Pf1N1B4_1540 0.47 +2.8 1,777,217 + Pf1N1B4_1540 0.47 -0.0 1,777,326 + Pf1N1B4_1540 0.67 +2.2 1,777,328 + Pf1N1B4_1540 0.68 -0.8 1,777,328 + Pf1N1B4_1540 0.68 -0.3 1,777,328 + Pf1N1B4_1540 0.68 +1.2 1,777,329 - Pf1N1B4_1540 0.68 -0.4 1,777,329 - Pf1N1B4_1540 0.68 -1.8 1,777,406 + Pf1N1B4_1540 0.83 +2.4 1,777,519 + -0.7 1,777,519 + -0.1 1,777,519 + +1.2 1,777,696 + Pf1N1B4_1541 0.32 -0.4 1,777,696 + Pf1N1B4_1541 0.32 -1.4 1,777,696 + Pf1N1B4_1541 0.32 +1.6 1,777,697 - +1.2 1,777,862 + Pf1N1B4_1541 0.59 +0.6 1,777,864 + Pf1N1B4_1541 0.59 -0.4 1,777,864 + Pf1N1B4_1541 0.59 +0.5 1,777,864 + Pf1N1B4_1541 0.59 -0.2 1,777,864 + Pf1N1B4_1541 0.59 -0.8 1,777,864 + Pf1N1B4_1541 0.59 +1.5 1,777,864 + Pf1N1B4_1541 0.59 +0.5 1,777,864 + Pf1N1B4_1541 0.59 -1.2 1,777,864 + Pf1N1B4_1541 0.59 +0.2 1,777,864 + Pf1N1B4_1541 0.59 -1.3 1,777,864 + Pf1N1B4_1541 0.59 +1.2 1,777,864 + Pf1N1B4_1541 0.59 -0.5 1,777,864 + Pf1N1B4_1541 0.59 -0.4 1,777,864 + Pf1N1B4_1541 0.59 +1.6 1,777,864 + Pf1N1B4_1541 0.59 +0.6 1,777,864 + Pf1N1B4_1541 0.59 +0.6 1,777,865 - Pf1N1B4_1541 0.59 -0.8 1,777,865 - Pf1N1B4_1541 0.59 +2.9 1,777,865 - Pf1N1B4_1541 0.59 +1.2 1,777,865 - -0.8 1,777,865 - Pf1N1B4_1541 0.59 +0.2 1,777,865 - Pf1N1B4_1541 0.59 -0.4 1,777,865 - Pf1N1B4_1541 0.59 +0.2 1,777,865 - Pf1N1B4_1541 0.59 +0.7 1,777,865 - Pf1N1B4_1541 0.59 +2.5 1,778,120 - +0.8 1,778,341 - Pf1N1B4_1542 0.40 -0.5 1,778,514 + +1.6 1,778,514 + Pf1N1B4_1542 0.71 +1.0 1,778,514 + Pf1N1B4_1542 0.71 +1.5 1,778,514 + Pf1N1B4_1542 0.71 +0.5 1,778,514 + Pf1N1B4_1542 0.71 +1.2 1,778,515 - Pf1N1B4_1542 0.71 +3.2 1,778,615 + Pf1N1B4_1542 0.88 -0.8 1,778,616 - Pf1N1B4_1542 0.89 +2.0 1,778,660 + -1.4 1,778,661 - +0.6 1,778,891 + Pf1N1B4_1543 0.20 +1.2 1,778,892 - Pf1N1B4_1543 0.20 -0.5 1,778,892 - Pf1N1B4_1543 0.20 -0.8 1,778,979 - Pf1N1B4_1543 0.28 +1.5 1,779,038 + Pf1N1B4_1543 0.34 +0.2 1,779,095 + Pf1N1B4_1543 0.39 -0.7 1,779,095 + Pf1N1B4_1543 0.39 +0.2 1,779,095 + Pf1N1B4_1543 0.39 +0.1 1,779,095 + Pf1N1B4_1543 0.39 -0.4 1,779,095 + Pf1N1B4_1543 0.39 +0.3 1,779,095 + Pf1N1B4_1543 0.39 -0.6 1,779,096 - Pf1N1B4_1543 0.39 +0.9 1,779,096 - Pf1N1B4_1543 0.39 +1.2 1,779,227 + Pf1N1B4_1543 0.51 +1.0 1,779,228 - Pf1N1B4_1543 0.51 +0.5 1,779,404 + Pf1N1B4_1543 0.68 +0.5 1,779,404 + Pf1N1B4_1543 0.68 +0.6 1,779,404 + Pf1N1B4_1543 0.68 +1.6 1,779,404 + Pf1N1B4_1543 0.68 +1.6 1,779,404 + Pf1N1B4_1543 0.68 +1.2 1,779,404 + Pf1N1B4_1543 0.68 -0.3 1,779,404 + Pf1N1B4_1543 0.68 -1.1 1,779,405 - Pf1N1B4_1543 0.68 -0.8 1,779,405 - Pf1N1B4_1543 0.68 +0.6 1,779,585 + Pf1N1B4_1543 0.85 +0.3 1,779,629 + Pf1N1B4_1543 0.89 -0.4 1,779,630 - Pf1N1B4_1543 0.89 +2.4 1,779,664 + +0.6 1,779,664 + -0.4 1,779,665 - +1.8
Or see this region's nucleotide sequence