Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1512

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1510 and Pf1N1B4_1511 are separated by 164 nucleotidesPf1N1B4_1511 and Pf1N1B4_1512 are separated by 4 nucleotidesPf1N1B4_1512 and Pf1N1B4_1513 overlap by 4 nucleotides Pf1N1B4_1510 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 1,742,911 to 1,743,336 _1510 Pf1N1B4_1511 - FIG027190: Putative transmembrane protein, at 1,743,501 to 1,744,067 _1511 Pf1N1B4_1512 - FIG021862: membrane protein, exporter, at 1,744,072 to 1,746,390 _1512 Pf1N1B4_1513 - FIG064705: cation/hydrogen antiporter, at 1,746,387 to 1,747,547 _1513 Position (kb) 1744 1745 1746 1747Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1743.115 kb on + strand, within Pf1N1B4_1510at 1743.116 kb on - strand, within Pf1N1B4_1510at 1743.116 kb on - strand, within Pf1N1B4_1510at 1743.116 kb on - strand, within Pf1N1B4_1510at 1743.116 kb on - strand, within Pf1N1B4_1510at 1743.116 kb on - strand, within Pf1N1B4_1510at 1743.119 kb on + strand, within Pf1N1B4_1510at 1743.119 kb on + strand, within Pf1N1B4_1510at 1743.120 kb on - strand, within Pf1N1B4_1510at 1743.120 kb on - strand, within Pf1N1B4_1510at 1743.120 kb on - strand, within Pf1N1B4_1510at 1743.120 kb on - strand, within Pf1N1B4_1510at 1743.120 kb on - strand, within Pf1N1B4_1510at 1743.120 kb on - strand, within Pf1N1B4_1510at 1743.197 kb on - strandat 1743.197 kb on - strand, within Pf1N1B4_1510at 1743.353 kb on + strandat 1743.354 kb on - strandat 1743.354 kb on - strandat 1743.431 kb on - strandat 1743.496 kb on + strandat 1743.497 kb on - strandat 1743.650 kb on - strand, within Pf1N1B4_1511at 1743.706 kb on - strand, within Pf1N1B4_1511at 1743.811 kb on + strand, within Pf1N1B4_1511at 1743.812 kb on - strand, within Pf1N1B4_1511at 1744.253 kb on + strandat 1744.603 kb on - strand, within Pf1N1B4_1512at 1744.771 kb on + strand, within Pf1N1B4_1512at 1744.771 kb on + strand, within Pf1N1B4_1512at 1744.771 kb on + strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1744.772 kb on - strand, within Pf1N1B4_1512at 1745.020 kb on + strand, within Pf1N1B4_1512at 1745.020 kb on + strand, within Pf1N1B4_1512at 1745.020 kb on + strand, within Pf1N1B4_1512at 1745.201 kb on - strand, within Pf1N1B4_1512at 1745.317 kb on + strand, within Pf1N1B4_1512at 1745.318 kb on - strand, within Pf1N1B4_1512at 1745.468 kb on - strand, within Pf1N1B4_1512at 1745.475 kb on + strand, within Pf1N1B4_1512at 1745.475 kb on + strand, within Pf1N1B4_1512at 1745.476 kb on - strand, within Pf1N1B4_1512at 1745.587 kb on + strand, within Pf1N1B4_1512at 1745.587 kb on + strand, within Pf1N1B4_1512at 1745.587 kb on + strand, within Pf1N1B4_1512at 1745.587 kb on + strand, within Pf1N1B4_1512at 1745.587 kb on + strand, within Pf1N1B4_1512at 1745.588 kb on - strand, within Pf1N1B4_1512at 1745.591 kb on + strand, within Pf1N1B4_1512at 1745.592 kb on - strand, within Pf1N1B4_1512at 1745.592 kb on - strand, within Pf1N1B4_1512at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.519 kb on + strand, within Pf1N1B4_1513at 1746.520 kb on - strand, within Pf1N1B4_1513at 1746.520 kb on - strand, within Pf1N1B4_1513at 1746.520 kb on - strandat 1746.520 kb on - strand, within Pf1N1B4_1513at 1746.520 kb on - strand, within Pf1N1B4_1513at 1746.520 kb on - strand, within Pf1N1B4_1513at 1746.693 kb on - strand, within Pf1N1B4_1513at 1746.804 kb on + strand, within Pf1N1B4_1513at 1746.804 kb on + strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.805 kb on - strand, within Pf1N1B4_1513at 1746.810 kb on + strand, within Pf1N1B4_1513at 1746.811 kb on - strand, within Pf1N1B4_1513at 1746.930 kb on - strand, within Pf1N1B4_1513at 1747.080 kb on + strand, within Pf1N1B4_1513at 1747.354 kb on + strand, within Pf1N1B4_1513

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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1,743,115 + Pf1N1B4_1510 0.48 -2.0
1,743,116 - Pf1N1B4_1510 0.48 -1.5
1,743,116 - Pf1N1B4_1510 0.48 -1.1
1,743,116 - Pf1N1B4_1510 0.48 +0.9
1,743,116 - Pf1N1B4_1510 0.48 -1.5
1,743,116 - Pf1N1B4_1510 0.48 +0.2
1,743,119 + Pf1N1B4_1510 0.49 -0.8
1,743,119 + Pf1N1B4_1510 0.49 +1.9
1,743,120 - Pf1N1B4_1510 0.49 -0.8
1,743,120 - Pf1N1B4_1510 0.49 -0.4
1,743,120 - Pf1N1B4_1510 0.49 +0.9
1,743,120 - Pf1N1B4_1510 0.49 -0.7
1,743,120 - Pf1N1B4_1510 0.49 +0.6
1,743,120 - Pf1N1B4_1510 0.49 -2.7
1,743,197 - +0.2
1,743,197 - Pf1N1B4_1510 0.67 -0.9
1,743,353 + -1.1
1,743,354 - -2.3
1,743,354 - +0.1
1,743,431 - +0.0
1,743,496 + -1.8
1,743,497 - -2.5
1,743,650 - Pf1N1B4_1511 0.26 -0.5
1,743,706 - Pf1N1B4_1511 0.36 -0.8
1,743,811 + Pf1N1B4_1511 0.55 -0.4
1,743,812 - Pf1N1B4_1511 0.55 -0.8
1,744,253 + -1.4
1,744,603 - Pf1N1B4_1512 0.23 -1.1
1,744,771 + Pf1N1B4_1512 0.30 -1.1
1,744,771 + Pf1N1B4_1512 0.30 -0.4
1,744,771 + Pf1N1B4_1512 0.30 -3.0
1,744,772 - Pf1N1B4_1512 0.30 -2.0
1,744,772 - Pf1N1B4_1512 0.30 +0.1
1,744,772 - Pf1N1B4_1512 0.30 -0.4
1,744,772 - Pf1N1B4_1512 0.30 -0.1
1,744,772 - Pf1N1B4_1512 0.30 -0.1
1,744,772 - Pf1N1B4_1512 0.30 -2.0
1,744,772 - Pf1N1B4_1512 0.30 -1.3
1,745,020 + Pf1N1B4_1512 0.41 -0.1
1,745,020 + Pf1N1B4_1512 0.41 -1.7
1,745,020 + Pf1N1B4_1512 0.41 -0.6
1,745,201 - Pf1N1B4_1512 0.49 -0.1
1,745,317 + Pf1N1B4_1512 0.54 -0.3
1,745,318 - Pf1N1B4_1512 0.54 -1.5
1,745,468 - Pf1N1B4_1512 0.60 -0.1
1,745,475 + Pf1N1B4_1512 0.61 +2.5
1,745,475 + Pf1N1B4_1512 0.61 -1.3
1,745,476 - Pf1N1B4_1512 0.61 -0.8
1,745,587 + Pf1N1B4_1512 0.65 -0.4
1,745,587 + Pf1N1B4_1512 0.65 -1.8
1,745,587 + Pf1N1B4_1512 0.65 -0.7
1,745,587 + Pf1N1B4_1512 0.65 -0.6
1,745,587 + Pf1N1B4_1512 0.65 -1.4
1,745,588 - Pf1N1B4_1512 0.65 +0.4
1,745,591 + Pf1N1B4_1512 0.66 -0.1
1,745,592 - Pf1N1B4_1512 0.66 -0.8
1,745,592 - Pf1N1B4_1512 0.66 +2.0
1,746,519 + Pf1N1B4_1513 0.11 +0.9
1,746,519 + Pf1N1B4_1513 0.11 +2.2
1,746,519 + Pf1N1B4_1513 0.11 -0.4
1,746,519 + Pf1N1B4_1513 0.11 -0.2
1,746,519 + Pf1N1B4_1513 0.11 +0.4
1,746,519 + Pf1N1B4_1513 0.11 +1.4
1,746,519 + Pf1N1B4_1513 0.11 -0.4
1,746,520 - Pf1N1B4_1513 0.11 +0.8
1,746,520 - Pf1N1B4_1513 0.11 -0.9
1,746,520 - +0.8
1,746,520 - Pf1N1B4_1513 0.11 -0.3
1,746,520 - Pf1N1B4_1513 0.11 -0.4
1,746,520 - Pf1N1B4_1513 0.11 -0.3
1,746,693 - Pf1N1B4_1513 0.26 -1.0
1,746,804 + Pf1N1B4_1513 0.36 -0.0
1,746,804 + Pf1N1B4_1513 0.36 -0.0
1,746,805 - Pf1N1B4_1513 0.36 -0.5
1,746,805 - Pf1N1B4_1513 0.36 +0.6
1,746,805 - Pf1N1B4_1513 0.36 -1.4
1,746,805 - Pf1N1B4_1513 0.36 -0.8
1,746,805 - Pf1N1B4_1513 0.36 +1.4
1,746,805 - Pf1N1B4_1513 0.36 +0.1
1,746,805 - Pf1N1B4_1513 0.36 -1.1
1,746,805 - Pf1N1B4_1513 0.36 +0.2
1,746,805 - Pf1N1B4_1513 0.36 -1.1
1,746,810 + Pf1N1B4_1513 0.36 -0.8
1,746,811 - Pf1N1B4_1513 0.37 -1.0
1,746,930 - Pf1N1B4_1513 0.47 -1.8
1,747,080 + Pf1N1B4_1513 0.60 +1.2
1,747,354 + Pf1N1B4_1513 0.83 -0.4

Or see this region's nucleotide sequence