Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1509 and Pf1N1B4_1510 overlap by 8 nucleotides Pf1N1B4_1510 and Pf1N1B4_1511 are separated by 164 nucleotides Pf1N1B4_1511 and Pf1N1B4_1512 are separated by 4 nucleotides
Pf1N1B4_1509 - Putative histidine ammonia-lyase protein, at 1,741,374 to 1,742,918
_1509
Pf1N1B4_1510 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 1,742,911 to 1,743,336
_1510
Pf1N1B4_1511 - FIG027190: Putative transmembrane protein, at 1,743,501 to 1,744,067
_1511
Pf1N1B4_1512 - FIG021862: membrane protein, exporter, at 1,744,072 to 1,746,390
_1512
Position (kb)
1743
1744
1745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1742.886 kb on - strand at 1742.916 kb on + strand at 1742.954 kb on + strand, within Pf1N1B4_1510 at 1743.002 kb on - strand, within Pf1N1B4_1510 at 1743.002 kb on - strand, within Pf1N1B4_1510 at 1743.002 kb on - strand, within Pf1N1B4_1510 at 1743.002 kb on - strand, within Pf1N1B4_1510 at 1743.010 kb on + strand, within Pf1N1B4_1510 at 1743.010 kb on + strand, within Pf1N1B4_1510 at 1743.011 kb on - strand, within Pf1N1B4_1510 at 1743.011 kb on - strand, within Pf1N1B4_1510 at 1743.011 kb on - strand, within Pf1N1B4_1510 at 1743.011 kb on - strand, within Pf1N1B4_1510 at 1743.011 kb on - strand, within Pf1N1B4_1510 at 1743.058 kb on + strand, within Pf1N1B4_1510 at 1743.059 kb on - strand, within Pf1N1B4_1510 at 1743.059 kb on - strand, within Pf1N1B4_1510 at 1743.059 kb on - strand, within Pf1N1B4_1510 at 1743.115 kb on + strand, within Pf1N1B4_1510 at 1743.116 kb on - strand, within Pf1N1B4_1510 at 1743.116 kb on - strand, within Pf1N1B4_1510 at 1743.116 kb on - strand, within Pf1N1B4_1510 at 1743.116 kb on - strand, within Pf1N1B4_1510 at 1743.116 kb on - strand, within Pf1N1B4_1510 at 1743.119 kb on + strand, within Pf1N1B4_1510 at 1743.119 kb on + strand, within Pf1N1B4_1510 at 1743.120 kb on - strand, within Pf1N1B4_1510 at 1743.120 kb on - strand, within Pf1N1B4_1510 at 1743.120 kb on - strand, within Pf1N1B4_1510 at 1743.120 kb on - strand, within Pf1N1B4_1510 at 1743.120 kb on - strand, within Pf1N1B4_1510 at 1743.120 kb on - strand, within Pf1N1B4_1510 at 1743.197 kb on - strand at 1743.197 kb on - strand, within Pf1N1B4_1510 at 1743.353 kb on + strand at 1743.354 kb on - strand at 1743.354 kb on - strand at 1743.431 kb on - strand at 1743.496 kb on + strand at 1743.497 kb on - strand at 1743.650 kb on - strand, within Pf1N1B4_1511 at 1743.706 kb on - strand, within Pf1N1B4_1511 at 1743.811 kb on + strand, within Pf1N1B4_1511 at 1743.812 kb on - strand, within Pf1N1B4_1511 at 1744.253 kb on + strand at 1744.603 kb on - strand, within Pf1N1B4_1512 at 1744.771 kb on + strand, within Pf1N1B4_1512 at 1744.771 kb on + strand, within Pf1N1B4_1512 at 1744.771 kb on + strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1744.772 kb on - strand, within Pf1N1B4_1512 at 1745.020 kb on + strand, within Pf1N1B4_1512 at 1745.020 kb on + strand, within Pf1N1B4_1512 at 1745.020 kb on + strand, within Pf1N1B4_1512
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,742,886 - -3.1 1,742,916 + +1.2 1,742,954 + Pf1N1B4_1510 0.10 -0.0 1,743,002 - Pf1N1B4_1510 0.21 +0.2 1,743,002 - Pf1N1B4_1510 0.21 -1.1 1,743,002 - Pf1N1B4_1510 0.21 +1.2 1,743,002 - Pf1N1B4_1510 0.21 -1.1 1,743,010 + Pf1N1B4_1510 0.23 +1.7 1,743,010 + Pf1N1B4_1510 0.23 -0.8 1,743,011 - Pf1N1B4_1510 0.23 -1.4 1,743,011 - Pf1N1B4_1510 0.23 +0.2 1,743,011 - Pf1N1B4_1510 0.23 -0.7 1,743,011 - Pf1N1B4_1510 0.23 -0.4 1,743,011 - Pf1N1B4_1510 0.23 -0.5 1,743,058 + Pf1N1B4_1510 0.35 -0.4 1,743,059 - Pf1N1B4_1510 0.35 +0.2 1,743,059 - Pf1N1B4_1510 0.35 -0.5 1,743,059 - Pf1N1B4_1510 0.35 +0.0 1,743,115 + Pf1N1B4_1510 0.48 -2.0 1,743,116 - Pf1N1B4_1510 0.48 -1.5 1,743,116 - Pf1N1B4_1510 0.48 -1.1 1,743,116 - Pf1N1B4_1510 0.48 +0.9 1,743,116 - Pf1N1B4_1510 0.48 -1.5 1,743,116 - Pf1N1B4_1510 0.48 +0.2 1,743,119 + Pf1N1B4_1510 0.49 -0.8 1,743,119 + Pf1N1B4_1510 0.49 +1.9 1,743,120 - Pf1N1B4_1510 0.49 -0.8 1,743,120 - Pf1N1B4_1510 0.49 -0.4 1,743,120 - Pf1N1B4_1510 0.49 +0.9 1,743,120 - Pf1N1B4_1510 0.49 -0.7 1,743,120 - Pf1N1B4_1510 0.49 +0.6 1,743,120 - Pf1N1B4_1510 0.49 -2.7 1,743,197 - +0.2 1,743,197 - Pf1N1B4_1510 0.67 -0.9 1,743,353 + -1.1 1,743,354 - -2.3 1,743,354 - +0.1 1,743,431 - +0.0 1,743,496 + -1.8 1,743,497 - -2.5 1,743,650 - Pf1N1B4_1511 0.26 -0.5 1,743,706 - Pf1N1B4_1511 0.36 -0.8 1,743,811 + Pf1N1B4_1511 0.55 -0.4 1,743,812 - Pf1N1B4_1511 0.55 -0.8 1,744,253 + -1.4 1,744,603 - Pf1N1B4_1512 0.23 -1.1 1,744,771 + Pf1N1B4_1512 0.30 -1.1 1,744,771 + Pf1N1B4_1512 0.30 -0.4 1,744,771 + Pf1N1B4_1512 0.30 -3.0 1,744,772 - Pf1N1B4_1512 0.30 -2.0 1,744,772 - Pf1N1B4_1512 0.30 +0.1 1,744,772 - Pf1N1B4_1512 0.30 -0.4 1,744,772 - Pf1N1B4_1512 0.30 -0.1 1,744,772 - Pf1N1B4_1512 0.30 -0.1 1,744,772 - Pf1N1B4_1512 0.30 -2.0 1,744,772 - Pf1N1B4_1512 0.30 -1.3 1,745,020 + Pf1N1B4_1512 0.41 -0.1 1,745,020 + Pf1N1B4_1512 0.41 -1.7 1,745,020 + Pf1N1B4_1512 0.41 -0.6
Or see this region's nucleotide sequence