Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1419 and Pf1N1B4_1420 are separated by 182 nucleotides Pf1N1B4_1420 and Pf1N1B4_1421 are separated by 107 nucleotides Pf1N1B4_1421 and Pf1N1B4_1422 are separated by 212 nucleotides Pf1N1B4_1422 and Pf1N1B4_1423 are separated by 3 nucleotides
Pf1N1B4_1419 - putative membrane protein, at 1,628,551 to 1,629,162
_1419
Pf1N1B4_1420 - 3'-to-5' oligoribonuclease (orn), at 1,629,345 to 1,629,887
_1420
Pf1N1B4_1421 - Ribosome small subunit-stimulated GTPase EngC, at 1,629,995 to 1,631,026
_1421
Pf1N1B4_1422 - hypothetical protein, at 1,631,239 to 1,631,646
_1422
Pf1N1B4_1423 - Peptidase C14, caspase catalytic subunit p20, at 1,631,650 to 1,632,609
_1423
Position (kb)
1629
1630
1631
1632 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1629.146 kb on + strand at 1629.146 kb on + strand at 1629.146 kb on + strand at 1629.147 kb on - strand at 1629.195 kb on - strand at 1629.202 kb on + strand at 1629.203 kb on - strand at 1629.237 kb on - strand at 1629.237 kb on - strand at 1629.274 kb on + strand at 1629.274 kb on + strand at 1629.274 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.281 kb on - strand at 1629.281 kb on - strand at 1629.281 kb on - strand at 1629.906 kb on - strand at 1629.906 kb on - strand at 1629.918 kb on + strand at 1629.944 kb on + strand at 1629.944 kb on + strand at 1629.965 kb on + strand at 1629.965 kb on + strand at 1631.021 kb on + strand at 1631.021 kb on + strand at 1631.022 kb on - strand at 1631.024 kb on + strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.121 kb on - strand at 1631.144 kb on - strand at 1631.144 kb on - strand at 1631.194 kb on + strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.248 kb on + strand at 1631.248 kb on + strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.467 kb on + strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.676 kb on + strand at 1631.677 kb on - strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.680 kb on - strand at 1631.680 kb on - strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1632.002 kb on + strand, within Pf1N1B4_1423 at 1632.024 kb on + strand, within Pf1N1B4_1423 at 1632.025 kb on - strand, within Pf1N1B4_1423
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,629,146 + -1.2 1,629,146 + -1.8 1,629,146 + -0.4 1,629,147 - +0.7 1,629,195 - +0.2 1,629,202 + +2.2 1,629,203 - -0.0 1,629,237 - -0.7 1,629,237 - -2.2 1,629,274 + +1.9 1,629,274 + -1.2 1,629,274 + -0.1 1,629,280 + -1.8 1,629,280 + +0.8 1,629,280 + +0.2 1,629,280 + -0.8 1,629,280 + -1.4 1,629,281 - -0.2 1,629,281 - -0.2 1,629,281 - -0.2 1,629,906 - +0.5 1,629,906 - +0.3 1,629,918 + +2.0 1,629,944 + +1.2 1,629,944 + +2.2 1,629,965 + -0.3 1,629,965 + +1.9 1,631,021 + -0.1 1,631,021 + +0.9 1,631,022 - -1.0 1,631,024 + -0.4 1,631,025 - -0.4 1,631,025 - +1.2 1,631,025 - +1.0 1,631,075 - +0.8 1,631,075 - -1.2 1,631,075 - +0.8 1,631,075 - -0.4 1,631,075 - -1.2 1,631,075 - +0.6 1,631,083 - -1.6 1,631,083 - -1.3 1,631,083 - -1.4 1,631,121 - -1.2 1,631,144 - -1.8 1,631,144 - -0.0 1,631,194 + -0.2 1,631,195 - +0.3 1,631,195 - -0.6 1,631,195 - +1.2 1,631,195 - +1.5 1,631,195 - -0.4 1,631,195 - +1.2 1,631,195 - +3.2 1,631,195 - +2.2 1,631,248 + -0.8 1,631,248 + -1.4 1,631,249 - -1.4 1,631,249 - +1.7 1,631,249 - +0.6 1,631,458 + Pf1N1B4_1422 0.54 +0.6 1,631,458 + Pf1N1B4_1422 0.54 -0.8 1,631,458 + Pf1N1B4_1422 0.54 +1.5 1,631,458 + Pf1N1B4_1422 0.54 +1.2 1,631,458 + Pf1N1B4_1422 0.54 -0.2 1,631,458 + Pf1N1B4_1422 0.54 +0.2 1,631,458 + Pf1N1B4_1422 0.54 -0.4 1,631,459 - Pf1N1B4_1422 0.54 +1.6 1,631,459 - Pf1N1B4_1422 0.54 +1.2 1,631,459 - Pf1N1B4_1422 0.54 +1.8 1,631,459 - Pf1N1B4_1422 0.54 -1.4 1,631,467 + Pf1N1B4_1422 0.56 +0.4 1,631,468 - Pf1N1B4_1422 0.56 +0.8 1,631,468 - Pf1N1B4_1422 0.56 -0.4 1,631,468 - Pf1N1B4_1422 0.56 +1.8 1,631,676 + +0.6 1,631,677 - -0.2 1,631,679 + -1.8 1,631,679 + +1.2 1,631,679 + -0.8 1,631,679 + +0.3 1,631,680 - -0.4 1,631,680 - +1.2 1,631,683 + +1.2 1,631,683 + +2.2 1,631,683 + +0.6 1,631,683 + -0.6 1,631,683 + -0.4 1,631,683 + -0.6 1,631,683 + +1.2 1,631,683 + -0.8 1,631,683 + -1.2 1,631,683 + +0.0 1,631,683 + +1.2 1,631,684 - +2.4 1,631,684 - -1.2 1,631,684 - +0.4 1,631,878 + Pf1N1B4_1423 0.24 +1.8 1,631,878 + Pf1N1B4_1423 0.24 -0.7 1,631,878 + Pf1N1B4_1423 0.24 +2.2 1,631,879 - Pf1N1B4_1423 0.24 -1.2 1,631,879 - Pf1N1B4_1423 0.24 +1.1 1,631,879 - Pf1N1B4_1423 0.24 +2.0 1,631,942 - Pf1N1B4_1423 0.30 +1.6 1,631,942 - Pf1N1B4_1423 0.30 -1.0 1,631,942 - Pf1N1B4_1423 0.30 -2.3 1,632,002 + Pf1N1B4_1423 0.37 -0.1 1,632,024 + Pf1N1B4_1423 0.39 +1.2 1,632,025 - Pf1N1B4_1423 0.39 -1.2
Or see this region's nucleotide sequence