Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1294 and Pf1N1B4_1295 are separated by 24 nucleotides Pf1N1B4_1295 and Pf1N1B4_1296 are separated by 257 nucleotides Pf1N1B4_1296 and Pf1N1B4_1297 are separated by 222 nucleotides Pf1N1B4_1297 and Pf1N1B4_1298 are separated by 291 nucleotides
Pf1N1B4_1294 - VgrG protein, at 1,475,067 to 1,476,122
_1294
Pf1N1B4_1295 - hypothetical protein, at 1,476,147 to 1,476,368
_1295
Pf1N1B4_1296 - Biosynthetic arginine decarboxylase (EC 4.1.1.19) (from data) , at 1,476,626 to 1,478,539
_1296
Pf1N1B4_1297 - Translation initiation factor SUI1-related protein, at 1,478,762 to 1,479,133
_1297
Pf1N1B4_1298 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 1,479,425 to 1,479,994
_1298
Position (kb)
1476
1477
1478
1479 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1475.890 kb on + strand, within Pf1N1B4_1294 at 1476.030 kb on + strand at 1476.031 kb on - strand at 1476.031 kb on - strand at 1476.129 kb on + strand at 1476.130 kb on - strand at 1476.226 kb on + strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.351 kb on + strand at 1476.351 kb on + strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.449 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.469 kb on + strand at 1476.470 kb on - strand at 1476.470 kb on - strand at 1476.481 kb on + strand at 1476.481 kb on + strand at 1476.482 kb on - strand at 1476.583 kb on + strand at 1476.584 kb on - strand at 1476.584 kb on - strand at 1476.622 kb on + strand at 1476.622 kb on + strand at 1476.623 kb on - strand at 1476.623 kb on - strand at 1476.639 kb on + strand at 1476.639 kb on + strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.765 kb on + strand at 1476.813 kb on + strand at 1476.814 kb on - strand at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.927 kb on + strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.954 kb on - strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.245 kb on + strand, within Pf1N1B4_1296 at 1477.245 kb on + strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.296 kb on + strand, within Pf1N1B4_1296 at 1477.296 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.587 kb on + strand, within Pf1N1B4_1296 at 1477.602 kb on + strand, within Pf1N1B4_1296 at 1477.602 kb on + strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.641 kb on + strand, within Pf1N1B4_1296 at 1477.642 kb on - strand, within Pf1N1B4_1296 at 1477.710 kb on + strand, within Pf1N1B4_1296 at 1477.710 kb on + strand, within Pf1N1B4_1296 at 1477.743 kb on + strand, within Pf1N1B4_1296 at 1477.743 kb on + strand, within Pf1N1B4_1296 at 1478.017 kb on + strand, within Pf1N1B4_1296 at 1478.018 kb on - strand, within Pf1N1B4_1296 at 1478.018 kb on - strand, within Pf1N1B4_1296 at 1478.232 kb on + strand, within Pf1N1B4_1296 at 1478.233 kb on - strand, within Pf1N1B4_1296 at 1478.292 kb on - strand, within Pf1N1B4_1296 at 1478.550 kb on + strand at 1478.552 kb on + strand at 1478.552 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.564 kb on - strand at 1478.564 kb on - strand at 1478.564 kb on - strand at 1478.564 kb on - strand at 1478.596 kb on + strand at 1478.596 kb on + strand at 1478.617 kb on - strand at 1478.617 kb on - strand at 1478.635 kb on + strand at 1478.635 kb on + strand at 1478.635 kb on + strand at 1478.635 kb on + strand at 1478.636 kb on - strand at 1478.661 kb on - strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1479.300 kb on - strand at 1479.341 kb on - strand at 1479.343 kb on + strand at 1479.344 kb on - strand at 1479.344 kb on - strand at 1479.349 kb on + strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.426 kb on + strand at 1479.426 kb on + strand at 1479.426 kb on + strand at 1479.426 kb on + strand at 1479.427 kb on - strand at 1479.427 kb on - strand at 1479.428 kb on + strand at 1479.429 kb on - strand at 1479.429 kb on - strand at 1479.473 kb on + strand at 1479.473 kb on + strand at 1479.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,475,890 + Pf1N1B4_1294 0.78 +1.7 1,476,030 + -0.4 1,476,031 - -0.9 1,476,031 - -1.7 1,476,129 + +0.1 1,476,130 - +0.7 1,476,226 + Pf1N1B4_1295 0.36 -0.1 1,476,227 - Pf1N1B4_1295 0.36 -0.4 1,476,227 - Pf1N1B4_1295 0.36 -0.6 1,476,351 + +1.1 1,476,351 + +1.0 1,476,352 - -0.9 1,476,352 - -0.7 1,476,352 - +0.9 1,476,352 - -0.9 1,476,449 + +0.1 1,476,462 + +0.5 1,476,462 + +0.3 1,476,462 + -0.3 1,476,462 + +0.6 1,476,462 + +2.1 1,476,469 + -1.9 1,476,470 - -1.1 1,476,470 - -1.0 1,476,481 + +0.6 1,476,481 + +1.1 1,476,482 - +0.5 1,476,583 + +0.6 1,476,584 - -0.4 1,476,584 - +0.6 1,476,622 + -0.9 1,476,622 + -2.7 1,476,623 - -1.2 1,476,623 - -0.3 1,476,639 + -1.2 1,476,639 + -0.9 1,476,640 - -2.6 1,476,640 - -1.1 1,476,640 - -2.4 1,476,640 - -2.2 1,476,640 - -0.4 1,476,765 + -1.4 1,476,813 + +0.7 1,476,814 - -0.7 1,476,837 + Pf1N1B4_1296 0.11 -0.4 1,476,837 + Pf1N1B4_1296 0.11 +0.1 1,476,837 + Pf1N1B4_1296 0.11 -1.6 1,476,838 - Pf1N1B4_1296 0.11 -1.1 1,476,838 - Pf1N1B4_1296 0.11 +0.1 1,476,927 + Pf1N1B4_1296 0.16 -1.0 1,476,928 - Pf1N1B4_1296 0.16 -2.0 1,476,928 - Pf1N1B4_1296 0.16 +0.3 1,476,954 - Pf1N1B4_1296 0.17 -0.2 1,476,990 + Pf1N1B4_1296 0.19 -1.8 1,476,990 + Pf1N1B4_1296 0.19 -1.3 1,476,991 - Pf1N1B4_1296 0.19 +1.4 1,476,991 - Pf1N1B4_1296 0.19 -2.4 1,476,991 - Pf1N1B4_1296 0.19 -0.1 1,477,146 + Pf1N1B4_1296 0.27 -1.7 1,477,146 + Pf1N1B4_1296 0.27 -1.7 1,477,146 + Pf1N1B4_1296 0.27 -0.4 1,477,146 + Pf1N1B4_1296 0.27 -1.6 1,477,146 + Pf1N1B4_1296 0.27 -1.0 1,477,146 + Pf1N1B4_1296 0.27 +0.1 1,477,146 + Pf1N1B4_1296 0.27 -1.9 1,477,147 - Pf1N1B4_1296 0.27 -1.4 1,477,147 - Pf1N1B4_1296 0.27 -0.9 1,477,147 - Pf1N1B4_1296 0.27 -1.6 1,477,147 - Pf1N1B4_1296 0.27 -0.2 1,477,147 - Pf1N1B4_1296 0.27 -0.4 1,477,147 - Pf1N1B4_1296 0.27 -0.2 1,477,147 - Pf1N1B4_1296 0.27 -0.1 1,477,147 - Pf1N1B4_1296 0.27 -0.2 1,477,147 - Pf1N1B4_1296 0.27 -0.9 1,477,147 - Pf1N1B4_1296 0.27 -0.1 1,477,245 + Pf1N1B4_1296 0.32 -0.8 1,477,245 + Pf1N1B4_1296 0.32 -0.1 1,477,246 - Pf1N1B4_1296 0.32 -0.8 1,477,246 - Pf1N1B4_1296 0.32 -0.1 1,477,246 - Pf1N1B4_1296 0.32 -1.2 1,477,246 - Pf1N1B4_1296 0.32 -2.2 1,477,246 - Pf1N1B4_1296 0.32 -1.4 1,477,296 + Pf1N1B4_1296 0.35 -0.2 1,477,296 + Pf1N1B4_1296 0.35 +1.1 1,477,320 + Pf1N1B4_1296 0.36 -0.6 1,477,320 + -0.2 1,477,320 + Pf1N1B4_1296 0.36 +1.1 1,477,320 + Pf1N1B4_1296 0.36 -2.7 1,477,321 - Pf1N1B4_1296 0.36 -1.4 1,477,321 - Pf1N1B4_1296 0.36 +0.1 1,477,321 - Pf1N1B4_1296 0.36 -0.1 1,477,321 - Pf1N1B4_1296 0.36 -0.9 1,477,321 - Pf1N1B4_1296 0.36 -2.2 1,477,321 - +0.1 1,477,338 + Pf1N1B4_1296 0.37 -0.9 1,477,338 + Pf1N1B4_1296 0.37 +0.4 1,477,338 + Pf1N1B4_1296 0.37 -0.9 1,477,587 + Pf1N1B4_1296 0.50 -1.2 1,477,602 + Pf1N1B4_1296 0.51 -1.4 1,477,602 + Pf1N1B4_1296 0.51 -2.1 1,477,603 - Pf1N1B4_1296 0.51 -2.7 1,477,603 - Pf1N1B4_1296 0.51 -1.2 1,477,603 - Pf1N1B4_1296 0.51 -0.5 1,477,603 - Pf1N1B4_1296 0.51 -0.6 1,477,641 + Pf1N1B4_1296 0.53 -2.1 1,477,642 - Pf1N1B4_1296 0.53 -0.0 1,477,710 + Pf1N1B4_1296 0.57 -0.4 1,477,710 + Pf1N1B4_1296 0.57 -0.7 1,477,743 + Pf1N1B4_1296 0.58 -0.4 1,477,743 + Pf1N1B4_1296 0.58 -0.9 1,478,017 + Pf1N1B4_1296 0.73 +1.1 1,478,018 - Pf1N1B4_1296 0.73 +0.6 1,478,018 - Pf1N1B4_1296 0.73 -1.3 1,478,232 + Pf1N1B4_1296 0.84 +0.4 1,478,233 - Pf1N1B4_1296 0.84 -0.4 1,478,292 - Pf1N1B4_1296 0.87 -2.2 1,478,550 + -1.3 1,478,552 + +0.0 1,478,552 + +0.1 1,478,563 + -0.6 1,478,563 + +0.1 1,478,563 + -2.0 1,478,563 + -0.2 1,478,563 + -2.7 1,478,564 - +1.4 1,478,564 - +1.1 1,478,564 - -0.1 1,478,564 - -2.0 1,478,596 + -0.7 1,478,596 + -0.2 1,478,617 - -2.3 1,478,617 - +0.9 1,478,635 + -1.7 1,478,635 + -0.7 1,478,635 + -2.4 1,478,635 + -1.3 1,478,636 - +0.4 1,478,661 - +0.2 1,478,696 + -1.2 1,478,696 + -0.4 1,478,696 + -1.6 1,478,696 + -0.2 1,478,696 + +1.1 1,478,697 - +0.7 1,478,697 - +0.6 1,478,697 - -2.0 1,478,697 - +1.6 1,478,697 - -2.2 1,479,300 - +0.9 1,479,341 - -2.2 1,479,343 + -0.9 1,479,344 - +1.6 1,479,344 - -0.9 1,479,349 + +2.4 1,479,350 - -0.4 1,479,350 - -1.7 1,479,350 - -1.4 1,479,350 - -0.7 1,479,350 - -1.4 1,479,350 - +1.1 1,479,426 + -1.2 1,479,426 + +0.4 1,479,426 + -0.2 1,479,426 + +0.0 1,479,427 - -0.4 1,479,427 - -0.4 1,479,428 + -1.0 1,479,429 - +1.7 1,479,429 - +0.6 1,479,473 + -1.0 1,479,473 + -1.2 1,479,474 - +0.3
Or see this region's nucleotide sequence