Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1279

Experiment: Carbenicillin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1278 and Pf1N1B4_1279 are separated by 10 nucleotidesPf1N1B4_1279 and Pf1N1B4_1280 overlap by 4 nucleotides Pf1N1B4_1278 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 1,458,634 to 1,459,914 _1278 Pf1N1B4_1279 - Flp pilus assembly protein TadB, at 1,459,925 to 1,460,809 _1279 Pf1N1B4_1280 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 1,460,806 to 1,461,696 _1280 Position (kb) 1459 1460 1461Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1459.252 kb on - strand, within Pf1N1B4_1278at 1459.415 kb on + strand, within Pf1N1B4_1278at 1459.416 kb on - strand, within Pf1N1B4_1278at 1459.777 kb on - strand, within Pf1N1B4_1278at 1459.792 kb on + strandat 1459.792 kb on + strandat 1459.792 kb on + strandat 1459.792 kb on + strandat 1459.792 kb on + strandat 1459.792 kb on + strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.793 kb on - strandat 1459.803 kb on + strandat 1459.809 kb on - strandat 1459.912 kb on + strandat 1459.912 kb on + strandat 1459.991 kb on + strandat 1459.992 kb on - strandat 1460.103 kb on + strand, within Pf1N1B4_1279at 1460.104 kb on - strand, within Pf1N1B4_1279at 1460.246 kb on + strand, within Pf1N1B4_1279at 1460.247 kb on - strand, within Pf1N1B4_1279at 1460.247 kb on - strand, within Pf1N1B4_1279at 1460.699 kb on + strand, within Pf1N1B4_1279at 1460.699 kb on + strand, within Pf1N1B4_1279at 1460.699 kb on + strand, within Pf1N1B4_1279at 1460.699 kb on + strand, within Pf1N1B4_1279at 1460.805 kb on + strandat 1460.806 kb on - strandat 1460.813 kb on + strandat 1461.020 kb on + strand, within Pf1N1B4_1280at 1461.020 kb on + strand, within Pf1N1B4_1280at 1461.021 kb on - strand, within Pf1N1B4_1280at 1461.089 kb on + strand, within Pf1N1B4_1280at 1461.090 kb on - strand, within Pf1N1B4_1280at 1461.327 kb on - strand, within Pf1N1B4_1280at 1461.350 kb on + strand, within Pf1N1B4_1280at 1461.350 kb on + strand, within Pf1N1B4_1280at 1461.351 kb on - strand, within Pf1N1B4_1280at 1461.351 kb on - strand, within Pf1N1B4_1280at 1461.351 kb on - strand, within Pf1N1B4_1280at 1461.383 kb on + strand, within Pf1N1B4_1280at 1461.479 kb on + strand, within Pf1N1B4_1280at 1461.479 kb on + strand, within Pf1N1B4_1280at 1461.480 kb on - strand, within Pf1N1B4_1280at 1461.480 kb on - strand, within Pf1N1B4_1280at 1461.577 kb on + strand, within Pf1N1B4_1280at 1461.577 kb on + strandat 1461.577 kb on + strand, within Pf1N1B4_1280at 1461.577 kb on + strand, within Pf1N1B4_1280at 1461.577 kb on + strand, within Pf1N1B4_1280at 1461.578 kb on - strand, within Pf1N1B4_1280at 1461.578 kb on - strand, within Pf1N1B4_1280at 1461.578 kb on - strand, within Pf1N1B4_1280at 1461.578 kb on - strand, within Pf1N1B4_1280at 1461.578 kb on - strand, within Pf1N1B4_1280at 1461.578 kb on - strand, within Pf1N1B4_1280at 1461.717 kb on + strandat 1461.784 kb on + strandat 1461.784 kb on + strandat 1461.784 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml
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1,459,252 - Pf1N1B4_1278 0.48 -2.0
1,459,415 + Pf1N1B4_1278 0.61 -0.8
1,459,416 - Pf1N1B4_1278 0.61 +0.6
1,459,777 - Pf1N1B4_1278 0.89 +0.3
1,459,792 + +0.7
1,459,792 + +1.2
1,459,792 + +1.7
1,459,792 + +0.6
1,459,792 + +0.2
1,459,792 + -3.4
1,459,793 - +0.4
1,459,793 - -0.2
1,459,793 - +4.5
1,459,793 - -0.4
1,459,793 - +0.2
1,459,793 - +0.3
1,459,793 - -1.2
1,459,793 - +0.9
1,459,803 + +0.5
1,459,809 - -0.2
1,459,912 + +1.9
1,459,912 + +1.3
1,459,991 + +0.9
1,459,992 - -2.0
1,460,103 + Pf1N1B4_1279 0.20 +0.1
1,460,104 - Pf1N1B4_1279 0.20 -0.3
1,460,246 + Pf1N1B4_1279 0.36 +1.1
1,460,247 - Pf1N1B4_1279 0.36 -0.9
1,460,247 - Pf1N1B4_1279 0.36 -1.9
1,460,699 + Pf1N1B4_1279 0.87 +0.4
1,460,699 + Pf1N1B4_1279 0.87 -1.7
1,460,699 + Pf1N1B4_1279 0.87 -1.4
1,460,699 + Pf1N1B4_1279 0.87 -0.7
1,460,805 + -0.0
1,460,806 - -0.9
1,460,813 + +0.6
1,461,020 + Pf1N1B4_1280 0.24 +1.8
1,461,020 + Pf1N1B4_1280 0.24 +0.7
1,461,021 - Pf1N1B4_1280 0.24 -1.3
1,461,089 + Pf1N1B4_1280 0.32 +0.1
1,461,090 - Pf1N1B4_1280 0.32 -1.2
1,461,327 - Pf1N1B4_1280 0.58 +1.1
1,461,350 + Pf1N1B4_1280 0.61 -0.4
1,461,350 + Pf1N1B4_1280 0.61 +0.9
1,461,351 - Pf1N1B4_1280 0.61 +2.4
1,461,351 - Pf1N1B4_1280 0.61 +0.2
1,461,351 - Pf1N1B4_1280 0.61 +0.1
1,461,383 + Pf1N1B4_1280 0.65 -0.2
1,461,479 + Pf1N1B4_1280 0.76 +0.5
1,461,479 + Pf1N1B4_1280 0.76 -0.4
1,461,480 - Pf1N1B4_1280 0.76 +1.1
1,461,480 - Pf1N1B4_1280 0.76 -0.2
1,461,577 + Pf1N1B4_1280 0.87 -0.2
1,461,577 + -0.1
1,461,577 + Pf1N1B4_1280 0.87 -0.2
1,461,577 + Pf1N1B4_1280 0.87 -0.4
1,461,577 + Pf1N1B4_1280 0.87 +0.1
1,461,578 - Pf1N1B4_1280 0.87 -1.4
1,461,578 - Pf1N1B4_1280 0.87 -0.9
1,461,578 - Pf1N1B4_1280 0.87 -0.2
1,461,578 - Pf1N1B4_1280 0.87 +1.1
1,461,578 - Pf1N1B4_1280 0.87 +0.9
1,461,578 - Pf1N1B4_1280 0.87 +2.1
1,461,717 + -0.9
1,461,784 + +1.1
1,461,784 + +0.0
1,461,784 + -1.7

Or see this region's nucleotide sequence