Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1226 and Pf1N1B4_1227 are separated by 565 nucleotides Pf1N1B4_1227 and Pf1N1B4_1228 are separated by 245 nucleotides
Pf1N1B4_1226 - FIG01201438: hypothetical protein, at 1,391,941 to 1,392,912
_1226
Pf1N1B4_1227 - FIG00954870: hypothetical protein, at 1,393,478 to 1,393,822
_1227
Pf1N1B4_1228 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 1,394,068 to 1,394,955
_1228
Position (kb)
1393
1394 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1392.844 kb on + strand at 1392.845 kb on - strand at 1392.845 kb on - strand at 1392.845 kb on - strand at 1392.845 kb on - strand at 1392.954 kb on - strand at 1392.993 kb on - strand at 1392.999 kb on + strand at 1393.003 kb on + strand at 1393.004 kb on - strand at 1393.004 kb on - strand at 1393.037 kb on + strand at 1393.060 kb on - strand at 1393.063 kb on - strand at 1393.063 kb on - strand at 1393.088 kb on + strand at 1393.144 kb on + strand at 1393.144 kb on + strand at 1393.181 kb on + strand at 1393.182 kb on - strand at 1393.188 kb on + strand at 1393.189 kb on - strand at 1393.214 kb on - strand at 1393.225 kb on + strand at 1393.227 kb on + strand at 1393.227 kb on + strand at 1393.227 kb on + strand at 1393.228 kb on - strand at 1393.228 kb on - strand at 1393.230 kb on + strand at 1393.253 kb on - strand at 1393.364 kb on + strand at 1393.364 kb on + strand at 1393.364 kb on + strand at 1393.387 kb on + strand at 1393.387 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.394 kb on - strand at 1393.489 kb on + strand at 1393.489 kb on + strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.500 kb on + strand at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.587 kb on - strand, within Pf1N1B4_1227 at 1393.619 kb on - strand, within Pf1N1B4_1227 at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.646 kb on + strand at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.647 kb on - strand at 1393.655 kb on + strand, within Pf1N1B4_1227 at 1393.656 kb on - strand, within Pf1N1B4_1227 at 1393.670 kb on + strand, within Pf1N1B4_1227 at 1393.692 kb on - strand, within Pf1N1B4_1227 at 1393.805 kb on + strand at 1393.806 kb on - strand at 1393.873 kb on + strand at 1393.873 kb on + strand at 1393.873 kb on + strand at 1393.874 kb on - strand at 1393.874 kb on - strand at 1393.958 kb on - strand at 1393.958 kb on - strand at 1393.978 kb on - strand at 1393.978 kb on - strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.145 kb on - strand at 1394.145 kb on - strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,392,844 + +1.5 1,392,845 - +0.7 1,392,845 - +1.1 1,392,845 - +0.7 1,392,845 - +1.1 1,392,954 - -0.2 1,392,993 - +1.7 1,392,999 + +0.0 1,393,003 + -0.4 1,393,004 - +2.5 1,393,004 - +2.5 1,393,037 + -0.3 1,393,060 - -1.2 1,393,063 - -1.7 1,393,063 - -1.2 1,393,088 + -0.4 1,393,144 + +1.9 1,393,144 + -0.2 1,393,181 + +2.1 1,393,182 - +0.9 1,393,188 + +1.5 1,393,189 - +1.5 1,393,214 - +0.5 1,393,225 + +1.1 1,393,227 + -1.5 1,393,227 + +1.1 1,393,227 + +1.1 1,393,228 - -1.5 1,393,228 - -0.5 1,393,230 + +1.5 1,393,253 - +1.1 1,393,364 + -2.0 1,393,364 + -0.9 1,393,364 + -1.0 1,393,387 + -0.0 1,393,387 + +0.5 1,393,393 + +1.4 1,393,393 + +0.8 1,393,393 + +0.1 1,393,393 + -0.1 1,393,393 + +0.6 1,393,393 + +0.1 1,393,393 + -2.0 1,393,393 + -0.1 1,393,394 - +2.3 1,393,489 + +3.1 1,393,489 + +0.6 1,393,490 - -0.5 1,393,490 - -0.1 1,393,490 - +1.1 1,393,490 - +0.4 1,393,500 + +0.3 1,393,586 + Pf1N1B4_1227 0.31 -1.0 1,393,586 + Pf1N1B4_1227 0.31 +1.5 1,393,586 + Pf1N1B4_1227 0.31 +1.1 1,393,587 - Pf1N1B4_1227 0.32 +1.7 1,393,619 - Pf1N1B4_1227 0.41 +0.6 1,393,646 + Pf1N1B4_1227 0.49 -2.2 1,393,646 + +0.1 1,393,646 + Pf1N1B4_1227 0.49 +0.5 1,393,646 + Pf1N1B4_1227 0.49 -2.8 1,393,647 - -0.5 1,393,655 + Pf1N1B4_1227 0.51 -0.5 1,393,656 - Pf1N1B4_1227 0.52 -1.2 1,393,670 + Pf1N1B4_1227 0.56 -1.9 1,393,692 - Pf1N1B4_1227 0.62 -1.2 1,393,805 + +2.1 1,393,806 - +1.2 1,393,873 + +0.1 1,393,873 + +0.1 1,393,873 + -0.1 1,393,874 - -1.4 1,393,874 - -0.1 1,393,958 - +0.3 1,393,958 - -0.5 1,393,978 - -0.2 1,393,978 - +0.5 1,394,144 + +1.0 1,394,144 + -0.5 1,394,144 + +0.2 1,394,144 + +0.5 1,394,145 - +1.1 1,394,145 - +0.7 1,394,303 + Pf1N1B4_1228 0.26 -0.2 1,394,303 + Pf1N1B4_1228 0.26 -0.4 1,394,303 + Pf1N1B4_1228 0.26 -2.3 1,394,303 + +1.5 1,394,303 + Pf1N1B4_1228 0.26 -0.5 1,394,304 - Pf1N1B4_1228 0.27 +0.4 1,394,304 - Pf1N1B4_1228 0.27 -1.7 1,394,304 - Pf1N1B4_1228 0.27 +0.6 1,394,304 - Pf1N1B4_1228 0.27 +0.1 1,394,304 - Pf1N1B4_1228 0.27 -2.3 1,394,304 - Pf1N1B4_1228 0.27 -2.0 1,394,304 - +0.5 1,394,304 - Pf1N1B4_1228 0.27 -1.0 1,394,304 - Pf1N1B4_1228 0.27 +0.1
Or see this region's nucleotide sequence