Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1149 and Pf1N1B4_1150 are separated by 290 nucleotides Pf1N1B4_1150 and Pf1N1B4_1151 are separated by 2 nucleotides Pf1N1B4_1151 and Pf1N1B4_1152 are separated by 64 nucleotides
Pf1N1B4_1149 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,294,529 to 1,296,559
_1149
Pf1N1B4_1150 - AmpE protein, at 1,296,850 to 1,297,686
_1150
Pf1N1B4_1151 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 1,297,689 to 1,298,258
_1151
Pf1N1B4_1152 - Thymidine phosphorylase (EC 2.4.2.4), at 1,298,323 to 1,300,524
_1152
Position (kb)
1296
1297
1298 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1295.946 kb on + strand, within Pf1N1B4_1149 at 1296.447 kb on + strand at 1296.447 kb on + strand at 1296.447 kb on + strand at 1296.447 kb on + strand at 1296.448 kb on - strand at 1296.448 kb on - strand at 1296.448 kb on - strand at 1296.544 kb on - strand at 1296.585 kb on - strand at 1296.590 kb on + strand at 1296.632 kb on + strand at 1296.640 kb on + strand at 1296.641 kb on - strand at 1296.641 kb on - strand at 1296.641 kb on - strand at 1296.643 kb on - strand at 1296.647 kb on + strand at 1296.648 kb on - strand at 1296.648 kb on - strand at 1296.648 kb on - strand at 1296.667 kb on + strand at 1296.668 kb on - strand at 1296.668 kb on - strand at 1296.672 kb on + strand at 1296.672 kb on + strand at 1296.672 kb on + strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.758 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.775 kb on - strand at 1296.775 kb on - strand at 1296.775 kb on - strand at 1296.851 kb on + strand at 1296.852 kb on - strand at 1297.260 kb on - strand at 1297.260 kb on - strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.324 kb on - strand, within Pf1N1B4_1150 at 1297.324 kb on - strand, within Pf1N1B4_1150 at 1297.430 kb on + strand, within Pf1N1B4_1150 at 1297.430 kb on + strand, within Pf1N1B4_1150 at 1297.431 kb on - strand, within Pf1N1B4_1150 at 1297.431 kb on - strand, within Pf1N1B4_1150 at 1297.472 kb on + strand, within Pf1N1B4_1150 at 1297.473 kb on - strand, within Pf1N1B4_1150 at 1297.473 kb on - strand, within Pf1N1B4_1150 at 1297.691 kb on - strand at 1297.691 kb on - strand at 1298.069 kb on - strand, within Pf1N1B4_1151 at 1298.489 kb on + strand at 1298.489 kb on + strand at 1298.489 kb on + strand at 1298.489 kb on + strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,295,946 + Pf1N1B4_1149 0.70 -0.2 1,296,447 + +2.7 1,296,447 + +0.5 1,296,447 + +0.8 1,296,447 + +1.9 1,296,448 - +1.1 1,296,448 - -0.9 1,296,448 - -1.9 1,296,544 - -0.2 1,296,585 - +1.1 1,296,590 + +2.1 1,296,632 + +2.1 1,296,640 + -0.9 1,296,641 - -0.1 1,296,641 - -0.5 1,296,641 - +0.1 1,296,643 - -0.9 1,296,647 + -0.5 1,296,648 - +1.1 1,296,648 - +1.1 1,296,648 - -0.7 1,296,667 + -0.9 1,296,668 - -0.5 1,296,668 - +1.3 1,296,672 + -1.5 1,296,672 + -0.2 1,296,672 + +1.3 1,296,673 - -0.5 1,296,673 - -1.5 1,296,673 - +1.4 1,296,673 - +0.3 1,296,673 - +1.3 1,296,758 + +1.9 1,296,774 + +1.1 1,296,774 + -0.5 1,296,774 + +0.1 1,296,774 + +0.9 1,296,774 + +1.1 1,296,775 - +1.1 1,296,775 - -1.5 1,296,775 - -0.9 1,296,851 + -0.0 1,296,852 - -1.5 1,297,260 - +2.1 1,297,260 - Pf1N1B4_1150 0.49 +0.3 1,297,323 + Pf1N1B4_1150 0.57 +1.4 1,297,323 + Pf1N1B4_1150 0.57 +1.5 1,297,323 + Pf1N1B4_1150 0.57 +0.1 1,297,323 + Pf1N1B4_1150 0.57 +1.1 1,297,324 - Pf1N1B4_1150 0.57 -0.2 1,297,324 - Pf1N1B4_1150 0.57 +0.3 1,297,430 + Pf1N1B4_1150 0.69 +1.2 1,297,430 + Pf1N1B4_1150 0.69 +0.1 1,297,431 - Pf1N1B4_1150 0.69 +0.3 1,297,431 - Pf1N1B4_1150 0.69 +1.3 1,297,472 + Pf1N1B4_1150 0.74 +2.8 1,297,473 - Pf1N1B4_1150 0.74 +1.1 1,297,473 - Pf1N1B4_1150 0.74 +0.6 1,297,691 - +2.1 1,297,691 - +1.3 1,298,069 - Pf1N1B4_1151 0.67 +0.9 1,298,489 + +1.1 1,298,489 + +0.1 1,298,489 + +0.9 1,298,489 + -0.9 1,298,490 - -0.2 1,298,490 - -0.5 1,298,490 - -1.9 1,298,490 - +0.1 1,298,490 - +0.1 1,298,490 - -0.5 1,298,490 - +0.7 1,298,490 - +1.1 1,298,490 - -1.9
Or see this region's nucleotide sequence