Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1131 and Pf1N1B4_1132 are separated by 20 nucleotides Pf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotides Pf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotides
Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241
_1131
Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155
_1132
Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821
_1133
Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975
_1134
Position (kb)
1271
1272 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1270.239 kb on + strand at 1270.239 kb on + strand at 1270.239 kb on + strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.278 kb on + strand at 1270.278 kb on + strand at 1270.278 kb on + strand at 1270.279 kb on - strand at 1270.279 kb on - strand at 1270.281 kb on + strand at 1270.281 kb on + strand at 1270.281 kb on + strand at 1270.282 kb on - strand at 1270.282 kb on - strand at 1270.282 kb on - strand at 1270.284 kb on + strand at 1270.285 kb on - strand at 1270.285 kb on - strand at 1270.285 kb on - strand at 1270.398 kb on - strand, within Pf1N1B4_1132 at 1270.415 kb on + strand, within Pf1N1B4_1132 at 1270.416 kb on - strand, within Pf1N1B4_1132 at 1270.523 kb on + strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.634 kb on + strand, within Pf1N1B4_1132 at 1270.634 kb on + strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.670 kb on + strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.998 kb on - strand, within Pf1N1B4_1132 at 1271.396 kb on + strand, within Pf1N1B4_1133 at 1271.397 kb on - strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1272.054 kb on + strand at 1272.054 kb on + strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.064 kb on + strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.211 kb on + strand, within Pf1N1B4_1134 at 1272.556 kb on - strand, within Pf1N1B4_1134 at 1272.667 kb on + strand, within Pf1N1B4_1134 at 1272.706 kb on + strand, within Pf1N1B4_1134 at 1272.754 kb on + strand, within Pf1N1B4_1134 at 1272.755 kb on - strand, within Pf1N1B4_1134 at 1272.755 kb on - strand, within Pf1N1B4_1134 at 1272.784 kb on + strand, within Pf1N1B4_1134 at 1272.784 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.802 kb on - strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.821 kb on - strand, within Pf1N1B4_1134 at 1272.821 kb on - strand, within Pf1N1B4_1134
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 1,270,239 + +1.1 1,270,239 + -0.9 1,270,239 + +0.0 1,270,240 - +1.4 1,270,240 - +0.1 1,270,240 - +0.1 1,270,240 - -0.5 1,270,278 + -0.5 1,270,278 + -1.7 1,270,278 + +0.3 1,270,279 - +1.1 1,270,279 - +0.1 1,270,281 + +0.4 1,270,281 + +0.7 1,270,281 + +0.9 1,270,282 - +1.3 1,270,282 - +1.8 1,270,282 - -1.2 1,270,284 + -0.6 1,270,285 - +0.5 1,270,285 - +0.6 1,270,285 - +0.4 1,270,398 - Pf1N1B4_1132 0.15 -0.1 1,270,415 + Pf1N1B4_1132 0.17 +2.3 1,270,416 - Pf1N1B4_1132 0.17 -0.9 1,270,523 + Pf1N1B4_1132 0.29 +3.1 1,270,524 - Pf1N1B4_1132 0.29 +0.8 1,270,524 - Pf1N1B4_1132 0.29 +0.6 1,270,524 - Pf1N1B4_1132 0.29 +0.5 1,270,634 + Pf1N1B4_1132 0.42 -0.1 1,270,634 + Pf1N1B4_1132 0.42 +1.9 1,270,635 - Pf1N1B4_1132 0.42 -0.2 1,270,635 - Pf1N1B4_1132 0.42 +0.3 1,270,635 - Pf1N1B4_1132 0.42 +2.1 1,270,635 - Pf1N1B4_1132 0.42 +0.8 1,270,670 + Pf1N1B4_1132 0.46 +1.7 1,270,854 - Pf1N1B4_1132 0.66 +2.4 1,270,854 - Pf1N1B4_1132 0.66 +1.9 1,270,896 - +1.7 1,270,896 - Pf1N1B4_1132 0.71 +0.3 1,270,896 - Pf1N1B4_1132 0.71 -1.7 1,270,955 + Pf1N1B4_1132 0.78 +2.1 1,270,955 + Pf1N1B4_1132 0.78 +0.1 1,270,955 + Pf1N1B4_1132 0.78 +1.5 1,270,955 + Pf1N1B4_1132 0.78 -1.7 1,270,955 + -0.9 1,270,955 + Pf1N1B4_1132 0.78 +1.1 1,270,955 + Pf1N1B4_1132 0.78 +0.1 1,270,955 + Pf1N1B4_1132 0.78 +1.4 1,270,955 + Pf1N1B4_1132 0.78 +2.1 1,270,956 - Pf1N1B4_1132 0.78 -0.5 1,270,956 - Pf1N1B4_1132 0.78 +0.2 1,270,956 - Pf1N1B4_1132 0.78 +1.4 1,270,956 - Pf1N1B4_1132 0.78 +0.1 1,270,967 + Pf1N1B4_1132 0.79 +0.1 1,270,967 + Pf1N1B4_1132 0.79 -0.9 1,270,967 + Pf1N1B4_1132 0.79 -1.2 1,270,998 - Pf1N1B4_1132 0.82 +1.7 1,271,396 + Pf1N1B4_1133 0.35 -1.1 1,271,397 - Pf1N1B4_1133 0.35 -0.9 1,271,483 + Pf1N1B4_1133 0.48 +0.7 1,271,483 + -1.2 1,271,483 + Pf1N1B4_1133 0.48 -1.9 1,271,483 + Pf1N1B4_1133 0.48 +1.1 1,271,483 + Pf1N1B4_1133 0.48 +0.3 1,271,484 - Pf1N1B4_1133 0.48 +0.5 1,271,484 - Pf1N1B4_1133 0.48 -0.1 1,271,484 - Pf1N1B4_1133 0.48 +2.7 1,271,484 - Pf1N1B4_1133 0.48 +0.4 1,271,484 - Pf1N1B4_1133 0.48 +0.8 1,271,484 - Pf1N1B4_1133 0.48 +0.1 1,271,679 + Pf1N1B4_1133 0.78 -0.9 1,271,679 + Pf1N1B4_1133 0.78 -0.2 1,271,680 - Pf1N1B4_1133 0.78 +0.6 1,271,680 - Pf1N1B4_1133 0.78 +1.1 1,271,680 - Pf1N1B4_1133 0.78 -1.2 1,272,054 + +1.5 1,272,054 + Pf1N1B4_1134 0.20 -0.5 1,272,055 - Pf1N1B4_1134 0.20 -0.1 1,272,055 - Pf1N1B4_1134 0.20 +0.6 1,272,064 + Pf1N1B4_1134 0.21 -1.5 1,272,065 - Pf1N1B4_1134 0.21 -0.2 1,272,065 - Pf1N1B4_1134 0.21 +0.2 1,272,211 + Pf1N1B4_1134 0.34 +0.7 1,272,556 - Pf1N1B4_1134 0.64 -0.1 1,272,667 + Pf1N1B4_1134 0.73 +0.3 1,272,706 + Pf1N1B4_1134 0.77 -1.9 1,272,754 + Pf1N1B4_1134 0.81 -1.5 1,272,755 - Pf1N1B4_1134 0.81 +0.0 1,272,755 - Pf1N1B4_1134 0.81 +1.1 1,272,784 + Pf1N1B4_1134 0.83 -0.1 1,272,784 + Pf1N1B4_1134 0.83 -1.5 1,272,797 + Pf1N1B4_1134 0.85 -0.1 1,272,797 + Pf1N1B4_1134 0.85 -1.2 1,272,797 + Pf1N1B4_1134 0.85 +1.6 1,272,797 + Pf1N1B4_1134 0.85 +0.2 1,272,797 + Pf1N1B4_1134 0.85 -0.7 1,272,797 + Pf1N1B4_1134 0.85 -0.0 1,272,797 + Pf1N1B4_1134 0.85 +0.2 1,272,797 + Pf1N1B4_1134 0.85 -0.5 1,272,797 + Pf1N1B4_1134 0.85 +2.9 1,272,797 + Pf1N1B4_1134 0.85 -1.2 1,272,797 + Pf1N1B4_1134 0.85 +0.1 1,272,798 - Pf1N1B4_1134 0.85 +0.7 1,272,798 - Pf1N1B4_1134 0.85 -0.9 1,272,798 - Pf1N1B4_1134 0.85 -0.1 1,272,798 - Pf1N1B4_1134 0.85 +1.7 1,272,798 - Pf1N1B4_1134 0.85 -0.6 1,272,798 - Pf1N1B4_1134 0.85 -0.7 1,272,798 - Pf1N1B4_1134 0.85 +1.7 1,272,801 + Pf1N1B4_1134 0.85 +1.1 1,272,801 + Pf1N1B4_1134 0.85 -0.5 1,272,801 + Pf1N1B4_1134 0.85 +1.1 1,272,801 + Pf1N1B4_1134 0.85 +0.9 1,272,801 + Pf1N1B4_1134 0.85 +0.5 1,272,802 - Pf1N1B4_1134 0.85 +0.5 1,272,820 + Pf1N1B4_1134 0.87 +1.4 1,272,820 + Pf1N1B4_1134 0.87 +1.7 1,272,820 + Pf1N1B4_1134 0.87 +0.9 1,272,820 + Pf1N1B4_1134 0.87 +0.1 1,272,821 - Pf1N1B4_1134 0.87 +0.7 1,272,821 - Pf1N1B4_1134 0.87 +0.8
Or see this region's nucleotide sequence