Experiment: Carbenicillin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_103 and Pf1N1B4_104 are separated by 493 nucleotides Pf1N1B4_104 and Pf1N1B4_105 overlap by 4 nucleotides Pf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotides Pf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotides
Pf1N1B4_103 - Histidine permease YuiF, at 108,555 to 109,871
_103
Pf1N1B4_104 - Sensor protein PhoQ (EC 2.7.13.3), at 110,365 to 111,678
_104
Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343
_105
Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628
_106
Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975
_107
Position (kb) 110
111
112 Strain fitness (log2 ratio) -2
-1
0
1
2 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.400 kb on - strand, within Pf1N1B4_103 at 109.471 kb on + strand, within Pf1N1B4_103 at 109.471 kb on + strand at 109.471 kb on + strand, within Pf1N1B4_103 at 109.472 kb on - strand at 109.472 kb on - strand, within Pf1N1B4_103 at 109.472 kb on - strand, within Pf1N1B4_103 at 109.473 kb on + strand, within Pf1N1B4_103 at 109.521 kb on + strand, within Pf1N1B4_103 at 109.522 kb on - strand, within Pf1N1B4_103 at 109.522 kb on - strand, within Pf1N1B4_103 at 109.834 kb on - strand at 109.861 kb on - strand at 109.942 kb on - strand at 109.942 kb on - strand at 109.976 kb on - strand at 109.976 kb on - strand at 109.978 kb on + strand at 110.134 kb on + strand at 110.135 kb on - strand at 110.135 kb on - strand at 110.135 kb on - strand at 110.238 kb on - strand at 110.443 kb on - strand at 110.715 kb on - strand, within Pf1N1B4_104 at 111.065 kb on - strand, within Pf1N1B4_104 at 111.249 kb on + strand, within Pf1N1B4_104 at 111.250 kb on - strand, within Pf1N1B4_104 at 111.251 kb on + strand at 111.251 kb on + strand, within Pf1N1B4_104 at 111.252 kb on - strand at 111.252 kb on - strand at 111.252 kb on - strand, within Pf1N1B4_104 at 111.252 kb on - strand, within Pf1N1B4_104 at 111.252 kb on - strand at 111.435 kb on + strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.441 kb on - strand, within Pf1N1B4_104 at 111.441 kb on - strand, within Pf1N1B4_104 at 111.996 kb on + strand, within Pf1N1B4_105 at 112.232 kb on - strand, within Pf1N1B4_105 at 112.364 kb on + strand at 112.364 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.369 kb on - strand at 112.395 kb on + strand, within Pf1N1B4_106 at 112.409 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand at 112.438 kb on + strand, within Pf1N1B4_106 at 112.444 kb on + strand, within Pf1N1B4_106 at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 2 mg/ml remove 109,399 + Pf1N1B4_103 0.64 -2.6 109,399 + Pf1N1B4_103 0.64 -1.2 109,399 + Pf1N1B4_103 0.64 +0.8 109,400 - Pf1N1B4_103 0.64 -0.5 109,471 + Pf1N1B4_103 0.70 +0.5 109,471 + +0.2 109,471 + Pf1N1B4_103 0.70 -1.0 109,472 - +2.0 109,472 - Pf1N1B4_103 0.70 -0.5 109,472 - Pf1N1B4_103 0.70 +1.3 109,473 + Pf1N1B4_103 0.70 +1.4 109,521 + Pf1N1B4_103 0.73 -0.8 109,522 - Pf1N1B4_103 0.73 -1.2 109,522 - Pf1N1B4_103 0.73 +1.4 109,834 - -1.5 109,861 - +1.1 109,942 - -0.0 109,942 - -1.2 109,976 - +0.8 109,976 - -1.2 109,978 + -0.2 110,134 + -2.0 110,135 - -1.5 110,135 - -0.8 110,135 - -2.4 110,238 - +2.0 110,443 - +0.6 110,715 - Pf1N1B4_104 0.27 -1.8 111,065 - Pf1N1B4_104 0.53 -1.4 111,249 + Pf1N1B4_104 0.67 +0.1 111,250 - Pf1N1B4_104 0.67 -1.2 111,251 + -0.2 111,251 + Pf1N1B4_104 0.67 -1.0 111,252 - -1.5 111,252 - +1.4 111,252 - Pf1N1B4_104 0.68 +0.5 111,252 - Pf1N1B4_104 0.68 -1.2 111,252 - +0.2 111,435 + Pf1N1B4_104 0.81 -0.2 111,436 - Pf1N1B4_104 0.82 -0.8 111,436 - Pf1N1B4_104 0.82 -1.2 111,436 - Pf1N1B4_104 0.82 -0.2 111,441 - Pf1N1B4_104 0.82 -0.6 111,441 - Pf1N1B4_104 0.82 -0.2 111,996 + Pf1N1B4_105 0.48 +1.8 112,232 - Pf1N1B4_105 0.83 +0.1 112,364 + +0.8 112,364 + -0.6 112,368 + +0.0 112,368 + +0.1 112,368 + +0.7 112,369 - -2.2 112,395 + Pf1N1B4_106 0.17 +0.2 112,409 + Pf1N1B4_106 0.22 -1.5 112,438 + Pf1N1B4_106 0.32 +1.1 112,438 + +0.2 112,438 + Pf1N1B4_106 0.32 +0.3 112,444 + Pf1N1B4_106 0.34 -0.4 112,668 + -0.3 112,668 + -0.3 112,668 + -0.2 112,668 + +0.3 112,668 + -0.2
Or see this region's nucleotide sequence