Experiment: Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19865 and HSERO_RS19870 are separated by 23 nucleotides HSERO_RS19870 and HSERO_RS19875 are separated by 90 nucleotides HSERO_RS19875 and HSERO_RS19880 are separated by 154 nucleotides
HSERO_RS19865: HSERO_RS19865 - glutaredoxin, at 4,560,263 to 4,560,574
_RS19865
HSERO_RS19870: HSERO_RS19870 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 4,560,598 to 4,561,200
_RS19870
HSERO_RS19875: HSERO_RS19875 - 3-oxoacyl-ACP reductase, at 4,561,291 to 4,562,058
_RS19875
HSERO_RS19880: HSERO_RS19880 - pyruvate dehydrogenase E1, at 4,562,213 to 4,564,894
_RS19880
Position (kb) 4561
4562
4563 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4561.268 kb on + strand at 4561.448 kb on + strand, within HSERO_RS19875 at 4561.767 kb on - strand, within HSERO_RS19875 at 4561.871 kb on + strand, within HSERO_RS19875 at 4561.916 kb on - strand, within HSERO_RS19875 at 4561.920 kb on + strand, within HSERO_RS19875 at 4561.928 kb on - strand, within HSERO_RS19875 at 4561.928 kb on - strand, within HSERO_RS19875 at 4561.951 kb on + strand, within HSERO_RS19875 at 4562.040 kb on - strand at 4562.069 kb on + strand at 4562.210 kb on + strand at 4562.218 kb on - strand at 4562.377 kb on + strand at 4562.484 kb on - strand, within HSERO_RS19880 at 4562.489 kb on - strand, within HSERO_RS19880 at 4562.723 kb on + strand, within HSERO_RS19880 at 4562.837 kb on - strand, within HSERO_RS19880 at 4562.839 kb on + strand, within HSERO_RS19880 at 4562.887 kb on - strand, within HSERO_RS19880 at 4562.966 kb on + strand, within HSERO_RS19880 at 4563.015 kb on + strand, within HSERO_RS19880
Per-strain Table
Position Strand Gene LocusTag Fraction Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB remove 4,561,268 + +0.6 4,561,448 + HSERO_RS19875 0.20 +0.2 4,561,767 - HSERO_RS19875 0.62 +1.4 4,561,871 + HSERO_RS19875 0.76 +0.8 4,561,916 - HSERO_RS19875 0.81 +0.3 4,561,920 + HSERO_RS19875 0.82 -0.6 4,561,928 - HSERO_RS19875 0.83 +1.4 4,561,928 - HSERO_RS19875 0.83 +0.1 4,561,951 + HSERO_RS19875 0.86 +2.0 4,562,040 - -0.2 4,562,069 + -2.0 4,562,210 + +0.2 4,562,218 - -0.1 4,562,377 + -1.0 4,562,484 - HSERO_RS19880 0.10 -0.5 4,562,489 - HSERO_RS19880 0.10 -2.4 4,562,723 + HSERO_RS19880 0.19 -0.7 4,562,837 - HSERO_RS19880 0.23 +0.3 4,562,839 + HSERO_RS19880 0.23 +0.4 4,562,887 - HSERO_RS19880 0.25 +1.4 4,562,966 + HSERO_RS19880 0.28 +0.1 4,563,015 + HSERO_RS19880 0.30 +0.0
Or see this region's nucleotide sequence