Experiment: Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02460 and HSERO_RS02465 are separated by 41 nucleotides HSERO_RS02465 and HSERO_RS02470 are separated by 123 nucleotides HSERO_RS02470 and HSERO_RS02475 are separated by 79 nucleotides
HSERO_RS02460: HSERO_RS02460 - UDP pyrophosphate phosphatase, at 537,114 to 537,986
_RS02460
HSERO_RS02465: HSERO_RS02465 - ATP-dependent DNA helicase RecQ, at 538,028 to 539,884
_RS02465
HSERO_RS02470: HSERO_RS02470 - tRNA (guanine-N7)-methyltransferase, at 540,008 to 540,727
_RS02470
HSERO_RS02475: HSERO_RS02475 - tRNA-Gly, at 540,807 to 540,880
_RS02475
Position (kb) 538
539
540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 537.036 kb on - strand at 537.190 kb on - strand at 537.200 kb on - strand at 537.636 kb on - strand, within HSERO_RS02460 at 537.759 kb on + strand, within HSERO_RS02460 at 537.823 kb on - strand, within HSERO_RS02460 at 537.842 kb on + strand, within HSERO_RS02460 at 538.137 kb on - strand at 538.137 kb on - strand at 538.175 kb on - strand at 538.213 kb on + strand at 538.213 kb on + strand at 538.383 kb on + strand, within HSERO_RS02465 at 538.383 kb on + strand at 538.391 kb on - strand, within HSERO_RS02465 at 538.393 kb on + strand, within HSERO_RS02465 at 538.393 kb on + strand, within HSERO_RS02465 at 538.401 kb on - strand, within HSERO_RS02465 at 538.431 kb on + strand, within HSERO_RS02465 at 538.439 kb on - strand, within HSERO_RS02465 at 538.439 kb on - strand, within HSERO_RS02465 at 538.504 kb on - strand, within HSERO_RS02465 at 538.772 kb on - strand, within HSERO_RS02465 at 538.847 kb on + strand, within HSERO_RS02465 at 538.859 kb on + strand, within HSERO_RS02465 at 538.895 kb on - strand, within HSERO_RS02465 at 538.989 kb on - strand, within HSERO_RS02465 at 539.071 kb on - strand, within HSERO_RS02465 at 539.154 kb on - strand, within HSERO_RS02465 at 539.216 kb on - strand, within HSERO_RS02465 at 539.299 kb on + strand, within HSERO_RS02465 at 539.744 kb on - strand at 539.824 kb on + strand at 539.847 kb on - strand at 540.032 kb on + strand at 540.395 kb on - strand, within HSERO_RS02470 at 540.415 kb on + strand, within HSERO_RS02470 at 540.428 kb on + strand, within HSERO_RS02470 at 540.433 kb on + strand, within HSERO_RS02470 at 540.441 kb on - strand, within HSERO_RS02470 at 540.441 kb on - strand, within HSERO_RS02470 at 540.500 kb on + strand at 540.696 kb on - strand at 540.757 kb on - strand at 540.764 kb on + strand at 540.822 kb on - strand, within HSERO_RS02475 at 540.835 kb on + strand, within HSERO_RS02475 at 540.863 kb on + strand, within HSERO_RS02475 at 540.869 kb on + strand, within HSERO_RS02475 at 540.871 kb on - strand, within HSERO_RS02475 at 540.876 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB remove 537,036 - -0.6 537,190 - -0.2 537,200 - +0.4 537,636 - HSERO_RS02460 0.60 -0.1 537,759 + HSERO_RS02460 0.74 +0.5 537,823 - HSERO_RS02460 0.81 +0.1 537,842 + HSERO_RS02460 0.83 +1.6 538,137 - +0.4 538,137 - +0.1 538,175 - +2.0 538,213 + +1.1 538,213 + -0.3 538,383 + HSERO_RS02465 0.19 +1.5 538,383 + +3.2 538,391 - HSERO_RS02465 0.20 +1.5 538,393 + HSERO_RS02465 0.20 -0.3 538,393 + HSERO_RS02465 0.20 -0.1 538,401 - HSERO_RS02465 0.20 -0.5 538,431 + HSERO_RS02465 0.22 +0.0 538,439 - HSERO_RS02465 0.22 +0.0 538,439 - HSERO_RS02465 0.22 -0.1 538,504 - HSERO_RS02465 0.26 +0.6 538,772 - HSERO_RS02465 0.40 +1.4 538,847 + HSERO_RS02465 0.44 -1.8 538,859 + HSERO_RS02465 0.45 +0.3 538,895 - HSERO_RS02465 0.47 +0.0 538,989 - HSERO_RS02465 0.52 +0.4 539,071 - HSERO_RS02465 0.56 +0.2 539,154 - HSERO_RS02465 0.61 -0.1 539,216 - HSERO_RS02465 0.64 +0.5 539,299 + HSERO_RS02465 0.68 +0.1 539,744 - -0.1 539,824 + -0.9 539,847 - -0.9 540,032 + +1.2 540,395 - HSERO_RS02470 0.54 -1.2 540,415 + HSERO_RS02470 0.57 -0.2 540,428 + HSERO_RS02470 0.58 +0.2 540,433 + HSERO_RS02470 0.59 -0.5 540,441 - HSERO_RS02470 0.60 -1.8 540,441 - HSERO_RS02470 0.60 -3.2 540,500 + +1.4 540,696 - -0.3 540,757 - -0.1 540,764 + -1.3 540,822 - HSERO_RS02475 0.20 +0.0 540,835 + HSERO_RS02475 0.38 +0.1 540,863 + HSERO_RS02475 0.76 -0.3 540,869 + HSERO_RS02475 0.84 -1.3 540,871 - HSERO_RS02475 0.86 +0.3 540,876 - +0.1
Or see this region's nucleotide sequence