Experiment: Chlorate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrC and lepB overlap by 45 nucleotides lepB and Synpcc7942_0488 are separated by 143 nucleotides Synpcc7942_0488 and aldA are separated by 20 nucleotides
Synpcc7942_0486: pyrC - dihydroorotase, at 472,319 to 473,554
pyrC
Synpcc7942_0487: lepB - thylakoidal processing peptidase. Serine peptidase. MEROPS family S26A, at 473,510 to 474,172
lepB
Synpcc7942_0488: Synpcc7942_0488 - dihydroorotase, at 474,316 to 475,644
_0488
Synpcc7942_0489: aldA - aldehyde dehydrogenase, at 475,665 to 477,044
aldA
Position (kb) 474
475
476 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 473.533 kb on + strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.600 kb on - strand, within lepB at 474.176 kb on + strand at 474.222 kb on - strand at 474.277 kb on + strand at 474.283 kb on + strand at 474.294 kb on - strand at 475.663 kb on + strand at 475.663 kb on - strand at 475.710 kb on + strand at 475.713 kb on - strand at 475.723 kb on - strand at 475.724 kb on - strand at 475.750 kb on + strand at 475.758 kb on - strand at 475.760 kb on + strand at 475.890 kb on - strand, within aldA at 475.905 kb on + strand, within aldA at 475.913 kb on - strand, within aldA at 475.913 kb on - strand, within aldA at 475.926 kb on + strand, within aldA at 475.934 kb on - strand, within aldA at 475.948 kb on + strand, within aldA at 475.948 kb on + strand, within aldA at 475.966 kb on - strand, within aldA at 476.078 kb on + strand, within aldA at 476.103 kb on + strand, within aldA at 476.103 kb on - strand, within aldA at 476.152 kb on - strand, within aldA at 476.162 kb on + strand, within aldA at 476.186 kb on + strand, within aldA at 476.194 kb on - strand, within aldA at 476.212 kb on + strand, within aldA at 476.212 kb on + strand, within aldA at 476.220 kb on - strand, within aldA at 476.220 kb on - strand, within aldA at 476.220 kb on - strand, within aldA at 476.222 kb on + strand, within aldA at 476.255 kb on + strand, within aldA at 476.268 kb on + strand, within aldA at 476.276 kb on - strand, within aldA at 476.286 kb on - strand, within aldA at 476.343 kb on - strand, within aldA at 476.347 kb on + strand, within aldA at 476.372 kb on + strand, within aldA at 476.380 kb on - strand, within aldA at 476.385 kb on - strand, within aldA at 476.399 kb on + strand, within aldA at 476.421 kb on + strand, within aldA at 476.478 kb on + strand, within aldA at 476.495 kb on + strand, within aldA at 476.498 kb on - strand, within aldA at 476.507 kb on - strand, within aldA at 476.541 kb on + strand, within aldA at 476.601 kb on + strand, within aldA at 476.614 kb on + strand, within aldA at 476.622 kb on - strand, within aldA at 476.637 kb on - strand, within aldA
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorate 10 mM remove 473,533 + +2.4 473,549 + -1.0 473,549 + +1.1 473,549 + -0.1 473,600 - lepB Synpcc7942_0487 0.14 -4.4 474,176 + -1.6 474,222 - +1.6 474,277 + -3.1 474,283 + -1.1 474,294 - -0.6 475,663 + -0.8 475,663 - +1.0 475,710 + -1.3 475,713 - +0.0 475,723 - -1.6 475,724 - -1.1 475,750 + -1.6 475,758 - +0.4 475,760 + -1.6 475,890 - aldA Synpcc7942_0489 0.16 +0.8 475,905 + aldA Synpcc7942_0489 0.17 -1.6 475,913 - aldA Synpcc7942_0489 0.18 -0.1 475,913 - aldA Synpcc7942_0489 0.18 +0.0 475,926 + aldA Synpcc7942_0489 0.19 +0.0 475,934 - aldA Synpcc7942_0489 0.19 -0.8 475,948 + aldA Synpcc7942_0489 0.21 -0.6 475,948 + aldA Synpcc7942_0489 0.21 -1.6 475,966 - aldA Synpcc7942_0489 0.22 +0.8 476,078 + aldA Synpcc7942_0489 0.30 -0.6 476,103 + aldA Synpcc7942_0489 0.32 -1.0 476,103 - aldA Synpcc7942_0489 0.32 -2.6 476,152 - aldA Synpcc7942_0489 0.35 -1.6 476,162 + aldA Synpcc7942_0489 0.36 -2.1 476,186 + aldA Synpcc7942_0489 0.38 +0.0 476,194 - aldA Synpcc7942_0489 0.38 -2.6 476,212 + aldA Synpcc7942_0489 0.40 +0.2 476,212 + aldA Synpcc7942_0489 0.40 -1.3 476,220 - aldA Synpcc7942_0489 0.40 -1.6 476,220 - aldA Synpcc7942_0489 0.40 -2.6 476,220 - aldA Synpcc7942_0489 0.40 -1.0 476,222 + aldA Synpcc7942_0489 0.40 +0.0 476,255 + aldA Synpcc7942_0489 0.43 -1.6 476,268 + aldA Synpcc7942_0489 0.44 +1.1 476,276 - aldA Synpcc7942_0489 0.44 +0.4 476,286 - aldA Synpcc7942_0489 0.45 +1.0 476,343 - aldA Synpcc7942_0489 0.49 +1.6 476,347 + aldA Synpcc7942_0489 0.49 -0.6 476,372 + aldA Synpcc7942_0489 0.51 -0.2 476,380 - aldA Synpcc7942_0489 0.52 -2.6 476,385 - aldA Synpcc7942_0489 0.52 +0.9 476,399 + aldA Synpcc7942_0489 0.53 -3.6 476,421 + aldA Synpcc7942_0489 0.55 -0.6 476,478 + aldA Synpcc7942_0489 0.59 -1.1 476,495 + aldA Synpcc7942_0489 0.60 +0.0 476,498 - aldA Synpcc7942_0489 0.60 -1.0 476,507 - aldA Synpcc7942_0489 0.61 +0.0 476,541 + aldA Synpcc7942_0489 0.63 -0.6 476,601 + aldA Synpcc7942_0489 0.68 -0.6 476,614 + aldA Synpcc7942_0489 0.69 -2.6 476,622 - aldA Synpcc7942_0489 0.69 -3.1 476,637 - aldA Synpcc7942_0489 0.70 +1.0
Or see this region's nucleotide sequence