Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_845 and Pf1N1B4_846 are separated by 232 nucleotides Pf1N1B4_846 and Pf1N1B4_847 are separated by 15 nucleotides
Pf1N1B4_845 - Membrane proteins related to metalloendopeptidases, at 973,712 to 974,533
_845
Pf1N1B4_846 - hypothetical protein, at 974,766 to 974,969
_846
Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664
_847
Position (kb)
974
975 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.862 kb on + strand, within Pf1N1B4_845 at 973.863 kb on - strand, within Pf1N1B4_845 at 973.863 kb on - strand, within Pf1N1B4_845 at 973.935 kb on + strand, within Pf1N1B4_845 at 973.935 kb on + strand at 974.003 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.461 kb on + strand at 974.461 kb on + strand at 974.462 kb on - strand at 974.462 kb on - strand at 974.656 kb on - strand at 974.656 kb on - strand at 974.729 kb on + strand at 974.915 kb on + strand, within Pf1N1B4_846 at 974.915 kb on + strand at 974.916 kb on - strand at 974.922 kb on + strand, within Pf1N1B4_846 at 974.972 kb on + strand at 974.972 kb on + strand at 975.016 kb on - strand at 975.016 kb on - strand at 975.246 kb on - strand, within Pf1N1B4_847 at 975.246 kb on - strand, within Pf1N1B4_847 at 975.326 kb on + strand, within Pf1N1B4_847 at 975.327 kb on - strand, within Pf1N1B4_847 at 975.327 kb on - strand at 975.327 kb on - strand, within Pf1N1B4_847 at 975.372 kb on + strand, within Pf1N1B4_847 at 975.372 kb on + strand, within Pf1N1B4_847 at 975.373 kb on - strand, within Pf1N1B4_847 at 975.373 kb on - strand, within Pf1N1B4_847 at 975.373 kb on - strand at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand at 975.861 kb on + strand, within Pf1N1B4_847 at 975.861 kb on + strand, within Pf1N1B4_847 at 975.862 kb on - strand, within Pf1N1B4_847 at 975.862 kb on - strand, within Pf1N1B4_847 at 975.862 kb on - strand at 975.862 kb on - strand, within Pf1N1B4_847 at 975.963 kb on + strand, within Pf1N1B4_847
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 973,775 + +1.2 973,775 + +0.7 973,775 + -1.1 973,775 + -2.5 973,775 + -0.4 973,775 + +0.2 973,775 + +0.2 973,775 + -2.1 973,775 + +1.4 973,775 + +0.4 973,775 + +0.7 973,775 + -0.1 973,775 + -1.4 973,776 - -0.8 973,776 - -0.8 973,776 - -1.6 973,776 - -1.4 973,776 - -0.8 973,776 - +1.6 973,776 - -0.7 973,776 - +0.2 973,776 - -0.0 973,776 - -0.4 973,862 + Pf1N1B4_845 0.18 -0.2 973,863 - Pf1N1B4_845 0.18 -1.6 973,863 - Pf1N1B4_845 0.18 -1.4 973,935 + Pf1N1B4_845 0.27 +0.2 973,935 + -0.4 974,003 + Pf1N1B4_845 0.35 +1.9 974,215 + Pf1N1B4_845 0.61 +0.9 974,215 + Pf1N1B4_845 0.61 -1.1 974,215 + Pf1N1B4_845 0.61 -0.6 974,216 - Pf1N1B4_845 0.61 +0.2 974,216 - Pf1N1B4_845 0.61 -1.1 974,216 - Pf1N1B4_845 0.61 -0.7 974,216 - Pf1N1B4_845 0.61 -1.1 974,216 - Pf1N1B4_845 0.61 -1.1 974,216 - Pf1N1B4_845 0.61 +0.0 974,304 - Pf1N1B4_845 0.72 -1.0 974,304 - Pf1N1B4_845 0.72 -2.0 974,304 - Pf1N1B4_845 0.72 -1.9 974,368 + Pf1N1B4_845 0.80 -2.0 974,368 + Pf1N1B4_845 0.80 +0.6 974,368 + Pf1N1B4_845 0.80 -0.5 974,369 - Pf1N1B4_845 0.80 -0.0 974,369 - Pf1N1B4_845 0.80 -1.4 974,461 + -1.1 974,461 + -1.8 974,462 - +0.4 974,462 - -1.8 974,656 - +0.8 974,656 - -0.8 974,729 + -0.8 974,915 + Pf1N1B4_846 0.73 +0.2 974,915 + -0.8 974,916 - -0.4 974,922 + Pf1N1B4_846 0.76 -1.8 974,972 + +1.0 974,972 + -1.3 975,016 - +1.2 975,016 - -1.4 975,246 - Pf1N1B4_847 0.16 -2.0 975,246 - Pf1N1B4_847 0.16 -1.1 975,326 + Pf1N1B4_847 0.20 -0.8 975,327 - Pf1N1B4_847 0.20 -2.0 975,327 - -0.8 975,327 - Pf1N1B4_847 0.20 -1.4 975,372 + Pf1N1B4_847 0.23 -1.4 975,372 + Pf1N1B4_847 0.23 -0.2 975,373 - Pf1N1B4_847 0.23 -1.4 975,373 - Pf1N1B4_847 0.23 -0.9 975,373 - -0.8 975,654 + Pf1N1B4_847 0.40 -1.1 975,654 + Pf1N1B4_847 0.40 -3.4 975,654 + Pf1N1B4_847 0.40 -0.4 975,654 + Pf1N1B4_847 0.40 -1.1 975,655 - Pf1N1B4_847 0.40 -1.6 975,655 - Pf1N1B4_847 0.40 +1.2 975,655 - Pf1N1B4_847 0.40 -1.1 975,655 - Pf1N1B4_847 0.40 -1.4 975,655 - Pf1N1B4_847 0.40 -3.0 975,655 - -0.8 975,861 + Pf1N1B4_847 0.52 -0.8 975,861 + Pf1N1B4_847 0.52 -0.9 975,862 - Pf1N1B4_847 0.52 -1.4 975,862 - Pf1N1B4_847 0.52 -1.4 975,862 - -0.4 975,862 - Pf1N1B4_847 0.52 -0.4 975,963 + Pf1N1B4_847 0.58 -1.1
Or see this region's nucleotide sequence