Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_753 and Pf1N1B4_754 are separated by 88 nucleotides
Pf1N1B4_753 - hypothetical protein, at 856,306 to 856,773
_753
Pf1N1B4_754 - hypothetical protein, at 856,862 to 857,212
_754
Position (kb) 856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 855.354 kb on - strand at 855.354 kb on - strand at 855.354 kb on - strand at 855.354 kb on - strand at 855.379 kb on + strand at 855.379 kb on + strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.380 kb on - strand at 855.436 kb on - strand at 855.446 kb on + strand at 855.447 kb on - strand at 855.447 kb on - strand at 855.454 kb on + strand at 855.455 kb on - strand at 855.455 kb on - strand at 855.498 kb on - strand at 855.543 kb on - strand at 855.543 kb on - strand at 855.543 kb on - strand at 855.578 kb on + strand at 855.579 kb on - strand at 855.579 kb on - strand at 855.606 kb on - strand at 855.707 kb on - strand at 855.766 kb on - strand at 855.958 kb on - strand at 856.114 kb on - strand at 856.114 kb on - strand at 856.450 kb on - strand, within Pf1N1B4_753 at 856.491 kb on + strand, within Pf1N1B4_753 at 856.491 kb on + strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand, within Pf1N1B4_753 at 856.492 kb on - strand at 856.557 kb on + strand, within Pf1N1B4_753 at 856.558 kb on - strand, within Pf1N1B4_753 at 856.576 kb on + strand at 856.781 kb on + strand at 856.806 kb on + strand at 856.806 kb on + strand at 856.806 kb on + strand at 856.806 kb on + strand at 856.807 kb on - strand at 856.807 kb on - strand at 856.807 kb on - strand at 856.873 kb on + strand at 856.873 kb on + strand at 856.874 kb on - strand at 856.874 kb on - strand at 856.874 kb on - strand at 856.874 kb on - strand at 856.874 kb on - strand at 857.033 kb on + strand, within Pf1N1B4_754 at 857.034 kb on - strand, within Pf1N1B4_754 at 857.135 kb on - strand, within Pf1N1B4_754 at 857.241 kb on + strand at 857.242 kb on - strand at 857.242 kb on - strand at 857.292 kb on - strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.355 kb on + strand at 857.356 kb on - strand at 857.356 kb on - strand at 857.356 kb on - strand at 857.356 kb on - strand at 857.422 kb on - strand at 857.516 kb on + strand at 857.665 kb on + strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.666 kb on - strand at 857.705 kb on - strand at 857.705 kb on - strand at 857.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 855,354 - -0.4 855,354 - -1.6 855,354 - +0.9 855,354 - -0.1 855,379 + +0.2 855,379 + -0.7 855,380 - +1.2 855,380 - +0.3 855,380 - -0.8 855,380 - -1.2 855,380 - +2.5 855,380 - +0.6 855,436 - +0.4 855,446 + -0.2 855,447 - -2.0 855,447 - +1.2 855,454 + +0.7 855,455 - -0.6 855,455 - -0.1 855,498 - +0.2 855,543 - +0.7 855,543 - -1.4 855,543 - +0.2 855,578 + -1.1 855,579 - +0.0 855,579 - +1.7 855,606 - -0.8 855,707 - +2.2 855,766 - -2.8 855,958 - -1.3 856,114 - +0.2 856,114 - +2.6 856,450 - Pf1N1B4_753 0.31 +0.9 856,491 + Pf1N1B4_753 0.40 -0.8 856,491 + Pf1N1B4_753 0.40 -1.8 856,492 - Pf1N1B4_753 0.40 -0.6 856,492 - Pf1N1B4_753 0.40 -0.4 856,492 - Pf1N1B4_753 0.40 -0.2 856,492 - Pf1N1B4_753 0.40 -1.2 856,492 - Pf1N1B4_753 0.40 -0.4 856,492 - +0.6 856,557 + Pf1N1B4_753 0.54 -1.2 856,558 - Pf1N1B4_753 0.54 -1.6 856,576 + -0.8 856,781 + +4.2 856,806 + -0.4 856,806 + +1.6 856,806 + +2.2 856,806 + -0.3 856,807 - -0.8 856,807 - -0.8 856,807 - -0.4 856,873 + -1.2 856,873 + -2.0 856,874 - +2.2 856,874 - -0.8 856,874 - +1.4 856,874 - -0.4 856,874 - -0.4 857,033 + Pf1N1B4_754 0.49 -0.3 857,034 - Pf1N1B4_754 0.49 +0.6 857,135 - Pf1N1B4_754 0.78 +1.2 857,241 + +2.0 857,242 - -2.4 857,242 - +1.6 857,292 - +2.7 857,355 + -1.4 857,355 + +1.2 857,355 + +1.2 857,355 + -0.8 857,355 + -1.2 857,355 + +1.2 857,355 + -0.0 857,356 - -0.6 857,356 - +0.6 857,356 - +2.7 857,356 - +0.9 857,422 - +0.8 857,516 + -0.8 857,665 + +2.0 857,666 - +0.2 857,666 - +0.2 857,666 - +1.7 857,666 - -0.4 857,666 - +0.6 857,705 - -0.4 857,705 - -1.6 857,708 - -1.0
Or see this region's nucleotide sequence