Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5991 and Pf1N1B4_5992 are separated by 300 nucleotides Pf1N1B4_5992 and Pf1N1B4_5993 overlap by 4 nucleotides Pf1N1B4_5993 and Pf1N1B4_5994 are separated by 14 nucleotides Pf1N1B4_5994 and Pf1N1B4_5995 overlap by 4 nucleotides
Pf1N1B4_5991 - Transcriptional regulator, AraC family, at 442,361 to 443,413
_5991
Pf1N1B4_5992 - Inner membrane protein, at 443,714 to 444,724
_5992
Pf1N1B4_5993 - Ferredoxin, 2Fe-2S, at 444,721 to 445,029
_5993
Pf1N1B4_5994 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 445,044 to 445,706
_5994
Pf1N1B4_5995 - Transporter, MFS superfamily, at 445,703 to 447,034
_5995
Position (kb) 443
444
445 Strain fitness (log2 ratio) -2
-1
0
1
2 at 443.013 kb on - strand, within Pf1N1B4_5991 at 443.075 kb on + strand, within Pf1N1B4_5991 at 443.075 kb on + strand, within Pf1N1B4_5991 at 443.075 kb on + strand at 443.076 kb on - strand, within Pf1N1B4_5991 at 443.076 kb on - strand, within Pf1N1B4_5991 at 443.243 kb on + strand at 443.465 kb on + strand at 443.482 kb on - strand at 443.534 kb on - strand at 443.924 kb on - strand, within Pf1N1B4_5992 at 444.515 kb on - strand, within Pf1N1B4_5992 at 444.757 kb on + strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.822 kb on - strand, within Pf1N1B4_5993 at 445.357 kb on - strand, within Pf1N1B4_5994 at 445.401 kb on - strand, within Pf1N1B4_5994 at 445.618 kb on - strand, within Pf1N1B4_5994 at 445.688 kb on + strand at 445.689 kb on - strand at 445.692 kb on + strand at 445.693 kb on - strand at 445.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 443,013 - Pf1N1B4_5991 0.62 -1.5 443,075 + Pf1N1B4_5991 0.68 +0.0 443,075 + Pf1N1B4_5991 0.68 -0.2 443,075 + -0.2 443,076 - Pf1N1B4_5991 0.68 +1.0 443,076 - Pf1N1B4_5991 0.68 +0.3 443,243 + +0.8 443,465 + -1.5 443,482 - -2.6 443,534 - -0.6 443,924 - Pf1N1B4_5992 0.21 -1.5 444,515 - Pf1N1B4_5992 0.79 -0.7 444,757 + Pf1N1B4_5993 0.12 -0.4 444,800 - Pf1N1B4_5993 0.26 +0.1 444,800 - Pf1N1B4_5993 0.26 +2.4 444,822 - Pf1N1B4_5993 0.33 +0.0 445,357 - Pf1N1B4_5994 0.47 -1.5 445,401 - Pf1N1B4_5994 0.54 +0.4 445,618 - Pf1N1B4_5994 0.87 -2.0 445,688 + +0.6 445,689 - -1.5 445,692 + -0.2 445,693 - -0.2 445,693 - -0.6
Or see this region's nucleotide sequence