Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5845 and Pf1N1B4_5846 are separated by 117 nucleotides Pf1N1B4_5846 and Pf1N1B4_5847 are separated by 101 nucleotides Pf1N1B4_5847 and Pf1N1B4_5848 are separated by 147 nucleotides Pf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides
Pf1N1B4_5845 - GMP synthase - Glutamine amidotransferase domain, at 279,680 to 280,321
_5845
Pf1N1B4_5846 - Rhodanese-related sulfurtransferase, at 280,439 to 280,873
_5846
Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949
_5847
Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852
_5848
Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337
_5849
Position (kb) 280
281
282 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 280.369 kb on - strand at 280.369 kb on - strand at 280.369 kb on - strand at 280.403 kb on + strand at 280.404 kb on - strand at 280.612 kb on - strand, within Pf1N1B4_5846 at 280.774 kb on - strand, within Pf1N1B4_5846 at 280.880 kb on + strand at 280.880 kb on + strand at 280.881 kb on - strand at 280.914 kb on - strand at 280.914 kb on - strand at 281.011 kb on + strand at 281.012 kb on - strand at 281.197 kb on - strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.425 kb on + strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.933 kb on + strand at 281.934 kb on - strand at 281.934 kb on - strand at 282.116 kb on + strand at 282.116 kb on + strand at 282.116 kb on + strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.120 kb on + strand at 282.121 kb on - strand at 282.121 kb on - strand at 282.242 kb on + strand, within Pf1N1B4_5848 at 282.243 kb on - strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand at 282.393 kb on + strand at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.495 kb on + strand, within Pf1N1B4_5848 at 282.496 kb on - strand at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.623 kb on + strand at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.693 kb on - strand, within Pf1N1B4_5848 at 282.888 kb on + strand at 282.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 280,369 - -1.8 280,369 - +0.8 280,369 - -0.8 280,403 + -0.8 280,404 - -1.5 280,612 - Pf1N1B4_5846 0.40 +1.0 280,774 - Pf1N1B4_5846 0.77 +1.4 280,880 + +2.4 280,880 + -0.8 280,881 - -0.8 280,914 - -0.8 280,914 - -0.2 281,011 + -0.6 281,012 - +0.1 281,197 - Pf1N1B4_5847 0.23 +3.1 281,402 + Pf1N1B4_5847 0.44 +1.8 281,402 + Pf1N1B4_5847 0.44 -1.2 281,402 + Pf1N1B4_5847 0.44 -2.0 281,402 + Pf1N1B4_5847 0.44 -1.8 281,402 + Pf1N1B4_5847 0.44 -0.1 281,403 - Pf1N1B4_5847 0.44 -2.0 281,403 - Pf1N1B4_5847 0.44 -1.5 281,403 - Pf1N1B4_5847 0.44 -0.4 281,403 - +0.8 281,425 + Pf1N1B4_5847 0.46 -0.2 281,425 + -1.5 281,425 + Pf1N1B4_5847 0.46 +0.4 281,933 + -0.8 281,934 - -0.8 281,934 - -0.5 282,116 + -1.2 282,116 + -1.5 282,116 + +0.8 282,117 - -1.2 282,117 - +1.2 282,117 - -1.2 282,117 - -0.1 282,117 - -0.5 282,117 - -0.4 282,120 + +0.6 282,121 - -0.2 282,121 - -1.2 282,242 + Pf1N1B4_5848 0.19 -0.8 282,243 - Pf1N1B4_5848 0.19 -0.3 282,393 + Pf1N1B4_5848 0.39 +0.6 282,393 + Pf1N1B4_5848 0.39 -0.7 282,393 + Pf1N1B4_5848 0.39 -1.5 282,393 + +0.8 282,393 + +0.8 282,393 + Pf1N1B4_5848 0.39 -1.2 282,393 + Pf1N1B4_5848 0.39 -0.0 282,394 - Pf1N1B4_5848 0.39 -2.2 282,394 - Pf1N1B4_5848 0.39 -1.3 282,394 - -0.2 282,394 - Pf1N1B4_5848 0.39 -0.2 282,394 - Pf1N1B4_5848 0.39 -1.5 282,394 - +1.2 282,394 - Pf1N1B4_5848 0.39 -0.2 282,495 + Pf1N1B4_5848 0.53 -1.4 282,496 - -1.2 282,503 + Pf1N1B4_5848 0.54 -1.4 282,503 + Pf1N1B4_5848 0.54 +0.1 282,503 + Pf1N1B4_5848 0.54 +0.4 282,503 + Pf1N1B4_5848 0.54 -1.2 282,503 + Pf1N1B4_5848 0.54 +0.5 282,504 - Pf1N1B4_5848 0.54 -0.6 282,504 - Pf1N1B4_5848 0.54 -1.5 282,623 + -0.8 282,692 + Pf1N1B4_5848 0.79 +0.2 282,692 + Pf1N1B4_5848 0.79 +1.2 282,693 - Pf1N1B4_5848 0.79 -1.4 282,888 + -2.8 282,889 - -2.0
Or see this region's nucleotide sequence