Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5802 and Pf1N1B4_5803 are separated by 226 nucleotides Pf1N1B4_5803 and Pf1N1B4_5804 are separated by 51 nucleotides Pf1N1B4_5804 and Pf1N1B4_5805 are separated by 10 nucleotides
Pf1N1B4_5802 - benABC operon transcriptional activator BenR, at 236,893 to 237,744
_5802
Pf1N1B4_5803 - Catechol 1,2-dioxygenase (EC 1.13.11.1), at 237,971 to 238,900
_5803
Pf1N1B4_5804 - Muconolactone isomerase (EC 5.3.3.4), at 238,952 to 239,242
_5804
Pf1N1B4_5805 - Muconate cycloisomerase (EC 5.5.1.1), at 239,253 to 240,542
_5805
Position (kb) 237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.072 kb on - strand, within Pf1N1B4_5802 at 237.113 kb on + strand, within Pf1N1B4_5802 at 237.192 kb on - strand, within Pf1N1B4_5802 at 237.192 kb on - strand, within Pf1N1B4_5802 at 237.294 kb on - strand at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.623 kb on + strand, within Pf1N1B4_5802 at 237.624 kb on - strand, within Pf1N1B4_5802 at 237.648 kb on + strand at 237.779 kb on + strand at 237.893 kb on + strand at 237.894 kb on - strand at 237.927 kb on + strand at 238.146 kb on + strand, within Pf1N1B4_5803 at 238.147 kb on - strand at 238.167 kb on + strand at 238.167 kb on + strand, within Pf1N1B4_5803 at 238.167 kb on + strand, within Pf1N1B4_5803 at 238.168 kb on - strand, within Pf1N1B4_5803 at 238.168 kb on - strand, within Pf1N1B4_5803 at 238.413 kb on + strand, within Pf1N1B4_5803 at 238.572 kb on + strand, within Pf1N1B4_5803 at 238.572 kb on + strand, within Pf1N1B4_5803 at 238.654 kb on - strand, within Pf1N1B4_5803 at 238.654 kb on - strand, within Pf1N1B4_5803 at 238.719 kb on + strand, within Pf1N1B4_5803 at 238.720 kb on - strand, within Pf1N1B4_5803 at 238.835 kb on - strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.923 kb on - strand at 239.016 kb on + strand, within Pf1N1B4_5804 at 239.085 kb on + strand at 239.085 kb on + strand, within Pf1N1B4_5804 at 239.094 kb on + strand, within Pf1N1B4_5804 at 239.094 kb on + strand, within Pf1N1B4_5804 at 239.114 kb on + strand, within Pf1N1B4_5804
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 237,046 - Pf1N1B4_5802 0.18 +0.1 237,046 - Pf1N1B4_5802 0.18 +0.2 237,046 - Pf1N1B4_5802 0.18 -1.4 237,072 - Pf1N1B4_5802 0.21 -0.3 237,113 + Pf1N1B4_5802 0.26 -0.5 237,192 - Pf1N1B4_5802 0.35 +1.1 237,192 - Pf1N1B4_5802 0.35 +0.2 237,294 - +0.8 237,507 - Pf1N1B4_5802 0.72 -1.5 237,507 - Pf1N1B4_5802 0.72 +0.6 237,507 - Pf1N1B4_5802 0.72 -2.0 237,507 - Pf1N1B4_5802 0.72 +1.0 237,564 - Pf1N1B4_5802 0.79 -2.0 237,564 - Pf1N1B4_5802 0.79 -1.8 237,564 - Pf1N1B4_5802 0.79 +2.4 237,564 - Pf1N1B4_5802 0.79 -0.2 237,623 + Pf1N1B4_5802 0.86 -2.4 237,624 - Pf1N1B4_5802 0.86 -0.4 237,648 + +0.8 237,779 + +0.4 237,893 + +1.0 237,894 - +0.1 237,927 + +0.8 238,146 + Pf1N1B4_5803 0.19 -0.2 238,147 - -0.2 238,167 + -1.5 238,167 + Pf1N1B4_5803 0.21 -0.9 238,167 + Pf1N1B4_5803 0.21 -3.2 238,168 - Pf1N1B4_5803 0.21 +0.8 238,168 - Pf1N1B4_5803 0.21 +2.3 238,413 + Pf1N1B4_5803 0.48 +0.8 238,572 + Pf1N1B4_5803 0.65 -1.8 238,572 + Pf1N1B4_5803 0.65 -2.5 238,654 - Pf1N1B4_5803 0.73 -2.0 238,654 - Pf1N1B4_5803 0.73 -1.4 238,719 + Pf1N1B4_5803 0.80 -1.8 238,720 - Pf1N1B4_5803 0.81 -2.0 238,835 - +1.8 238,918 + -3.3 238,918 + -1.2 238,923 - -0.0 239,016 + Pf1N1B4_5804 0.22 -2.4 239,085 + +0.8 239,085 + Pf1N1B4_5804 0.46 -0.8 239,094 + Pf1N1B4_5804 0.49 -1.5 239,094 + Pf1N1B4_5804 0.49 -0.0 239,114 + Pf1N1B4_5804 0.56 -0.3
Or see this region's nucleotide sequence