Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5791 and Pf1N1B4_5792 are separated by 74 nucleotides Pf1N1B4_5792 and Pf1N1B4_5793 are separated by 99 nucleotides
Pf1N1B4_5791 - hypothetical protein, at 229,111 to 229,446
_5791
Pf1N1B4_5792 - hypothetical protein, at 229,521 to 229,757
_5792
Pf1N1B4_5793 - Protein RtcB, at 229,857 to 231,083
_5793
Position (kb) 229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 228.127 kb on + strand at 228.127 kb on + strand at 228.148 kb on + strand at 228.149 kb on - strand at 228.170 kb on + strand at 228.171 kb on - strand at 228.178 kb on + strand at 228.185 kb on - strand at 228.272 kb on + strand at 228.273 kb on - strand at 228.334 kb on + strand at 228.334 kb on + strand at 228.334 kb on + strand at 228.334 kb on + strand at 228.334 kb on + strand at 228.334 kb on + strand at 228.335 kb on - strand at 228.335 kb on - strand at 228.335 kb on - strand at 228.335 kb on - strand at 228.335 kb on - strand at 228.335 kb on - strand at 228.346 kb on - strand at 228.346 kb on - strand at 228.346 kb on - strand at 228.367 kb on + strand at 228.367 kb on + strand at 228.367 kb on + strand at 228.422 kb on + strand at 228.423 kb on - strand at 228.423 kb on - strand at 228.605 kb on + strand at 228.606 kb on - strand at 228.606 kb on - strand at 228.665 kb on - strand at 229.095 kb on - strand at 229.095 kb on - strand at 229.095 kb on - strand at 229.095 kb on - strand at 229.173 kb on - strand, within Pf1N1B4_5791 at 229.306 kb on + strand at 229.316 kb on - strand, within Pf1N1B4_5791 at 229.362 kb on + strand, within Pf1N1B4_5791 at 229.522 kb on + strand at 229.522 kb on + strand at 229.524 kb on + strand at 229.592 kb on - strand, within Pf1N1B4_5792 at 229.592 kb on - strand at 229.609 kb on + strand, within Pf1N1B4_5792 at 229.797 kb on - strand at 229.896 kb on + strand at 229.896 kb on + strand at 229.897 kb on - strand at 229.897 kb on - strand at 229.921 kb on + strand at 229.922 kb on - strand at 229.922 kb on - strand at 229.945 kb on + strand at 229.946 kb on - strand at 229.946 kb on - strand at 229.946 kb on - strand at 230.236 kb on + strand, within Pf1N1B4_5793 at 230.236 kb on + strand, within Pf1N1B4_5793 at 230.237 kb on - strand, within Pf1N1B4_5793 at 230.237 kb on - strand, within Pf1N1B4_5793 at 230.237 kb on - strand, within Pf1N1B4_5793 at 230.377 kb on + strand at 230.377 kb on + strand, within Pf1N1B4_5793
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 228,127 + -0.2 228,127 + -0.2 228,148 + +1.8 228,149 - -1.2 228,170 + -0.4 228,171 - +1.8 228,178 + +0.2 228,185 - +1.0 228,272 + -1.2 228,273 - -1.1 228,334 + -1.5 228,334 + -2.8 228,334 + +0.2 228,334 + -0.2 228,334 + +0.4 228,334 + +0.1 228,335 - +0.8 228,335 - -0.8 228,335 - -0.6 228,335 - +0.5 228,335 - -1.5 228,335 - +0.8 228,346 - -2.4 228,346 - -2.2 228,346 - -0.8 228,367 + +0.9 228,367 + -0.2 228,367 + -2.4 228,422 + -1.5 228,423 - +1.1 228,423 - -1.1 228,605 + +1.0 228,606 - +0.3 228,606 - +0.2 228,665 - -0.2 229,095 - -0.3 229,095 - -0.1 229,095 - +0.8 229,095 - +0.4 229,173 - Pf1N1B4_5791 0.18 -2.0 229,306 + -0.2 229,316 - Pf1N1B4_5791 0.61 -1.2 229,362 + Pf1N1B4_5791 0.75 -0.5 229,522 + +0.4 229,522 + -0.8 229,524 + +0.8 229,592 - Pf1N1B4_5792 0.30 +0.4 229,592 - +1.8 229,609 + Pf1N1B4_5792 0.37 -0.2 229,797 - +1.6 229,896 + -0.2 229,896 + -2.2 229,897 - +0.1 229,897 - +0.2 229,921 + +2.8 229,922 - -1.0 229,922 - +1.8 229,945 + -1.3 229,946 - -0.7 229,946 - +0.4 229,946 - +0.2 230,236 + Pf1N1B4_5793 0.31 +0.2 230,236 + Pf1N1B4_5793 0.31 -0.4 230,237 - Pf1N1B4_5793 0.31 -1.7 230,237 - Pf1N1B4_5793 0.31 -2.0 230,237 - Pf1N1B4_5793 0.31 +0.6 230,377 + +0.8 230,377 + Pf1N1B4_5793 0.42 +1.4
Or see this region's nucleotide sequence